Produção de oleato de decila utilizando a lipase de Geotrichum candidum

• Ano de defesa: 2021
• Autor(a) principal: Santos, Luís Fernando Da Silva
• Orientador(a): Hirata, Daniela Battaglia
• Banca de defesa: Freitas, Larissa De, Angelotti, Joelise De Alencar Figueira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Alfenas
• Programa de Pós-Graduação: Programa de Pós-graduação em Biotecnologia
• Departamento: Instituto de Ciências Biomédicas
• País: Brasil
• Palavras-chave em Português: Lipase; Fungo; Imobilização; Otimização; Esterificação.
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS
• Link de acesso: https://repositorio.unifal-mg.edu.br/handle/123456789/1792

The present work aimed to produce the decyl oleate ester by esterification reaction using the lipase of Geotrichum candidum (LGC) immobilized via physical adsorption on a support prepared from agro-industrial waste as a biocatalyst. Decyl oleate is an ester used in a variety of cosmetics and personal care products, and its synthesis via enzymatic catalysis is of great interest to the cosmetic industry, as the processes catalyzed by enzymes are considered natural and less aggressive to the environment. environment and, in addition, they provide a single final product, reducing additional expenses with post- reaction purification processes. Lipases are enzymes that exhibit stereoselectivity, chemoselectivity and regioselectivity for the substrate. These properties make them extremely versatile and very attractive for different applications, including the cosmetic, oleochemical, food, detergent and pharmaceutical industry. In this work, the lipase of Geotrichum candidum (LGC) was produced by submerged fermentation at 30.0 oC, pH 7.0 and under agitation of 250 rpm for 48h and purified by precipitation with organic solvent. Then, it was immobilized by physical adsorption on different supports from agro- industrial residues, such as activated carbon of coffee straw and cane straw, rice straw silica functionalized with the phenyl group and with the vinyl group and also using a magnetic nanoparticle support, in the sequence the characterization of the biocatalytic properties of the derivatives obtained was carried out. Derivatives were also evaluated for their hydrophobicity using the Bengal Rose dye. Among the evaluated supports, the one that presented the best activity of the derivative was the silica support, obtained from rice straw and functionalized with the phenyl groups (phenyl-silica). The effect of the protein loading was evaluated to support phenylsilica, and the derivative with a loading of 8.13 mg / g was selected for the subsequent esterification step. The decyl oleate production process was optimized using a central rotational composite design (DCCR) for two variables: the concentration of biocatalyst offered and the reaction temperature. The optimum conditions for the production of the emollient were established for 15% (w / v) of biocatalyst and the reaction temperature of 50 ° C. The planning validation was performed under the best experimental conditions, with its maximum conversion observed in approximately 120 min (78%). The prepared biocatalyst maintained 73.3% of its initial activity after 6 consecutive cycles of 2 h of reaction each.