Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico

Detalhes bibliográficos
Ano de defesa: 2019
Autor(a) principal: Portugal, Joice Aparecida De Novais lattes
Orientador(a): Souza, Thiago Corrêa De lattes
Banca de defesa: Silva, Adriano Bortolloti Da, Rodrigues, Luiz Carlos Almeida
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Alfenas
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Ambientais
Departamento: Instituto de Ciências da Natureza
País: Brasil
Palavras-chave em Português:
Área do conhecimento CNPq:
Link de acesso: https://repositorio.unifal-mg.edu.br/handle/123456789/1809
Resumo: In agriculture one of the limiting factors of plant development that directly affects the final production result is water availability. With the constant climate changes, it is necessary to carry out studies to discover new technologies to minimize water stress caused on plants, especially in the germination and growth, flowering and fruit filling phase. This work aimed to analyze the application of new trehalose-derived disaccharides in maize plants by checking the drought tolerance induction capacity. The experiment was carried out in a greenhouse using BRS 1030 hybrid corn sensitive to water deficit. Water stress was applied when the plants reached vegetative stage V6 and was imposed for 12 days, with foliar application of the mixture of derivatives (30mM) on the first and tenth day of water deficit imposition. The photosynthetic efficiency analyzes of chlorophyll a (ETRmax, Ik and α) were performed on the first and last day of water deficit imposition and 12 hours after rehydration, obtaining fast light curves through a fluorimeter. Leaf collections for biochemical analysis were performed at the beginning and end of water deficit and 12 hours after plant rehydration. Lipid peroxidation through MDA contents and antioxidant enzymatic activity SOD, APX, CAT and POD were evaluated. At the end of the experiment the leaves and roots were collected to analyze the concentrations of reducing sugars, total soluble sugars, starch, protein, proline and phenolic compounds. It was concluded that the exogenous application of the trehalose derivatives mixture proved to be efficient in mitigating damage caused by water deficit through the activation of antioxidant enzymes (SOD, APX and POD), besides promoting the accumulation of sugars, proline and phenolic compounds and improving photosynthetic efficiency in the BRS 1030 maize hybrid
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spelling Portugal, Joice Aparecida De Novaishttp://lattes.cnpq.br/2834667104138232Souza, Kamila Rezende Dázio DeSilva, Adriano Bortolloti DaRodrigues, Luiz Carlos AlmeidaSouza, Thiago Corrêa Dehttp://lattes.cnpq.br/21718284641994332021-06-15T12:37:28Z2019-12-20PORTUGAL, Joice Aparecida de Novais. Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico. 2019. 55 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Alfenas, Alfenas, MG, 2019.https://repositorio.unifal-mg.edu.br/handle/123456789/1809In agriculture one of the limiting factors of plant development that directly affects the final production result is water availability. With the constant climate changes, it is necessary to carry out studies to discover new technologies to minimize water stress caused on plants, especially in the germination and growth, flowering and fruit filling phase. This work aimed to analyze the application of new trehalose-derived disaccharides in maize plants by checking the drought tolerance induction capacity. The experiment was carried out in a greenhouse using BRS 1030 hybrid corn sensitive to water deficit. Water stress was applied when the plants reached vegetative stage V6 and was imposed for 12 days, with foliar application of the mixture of derivatives (30mM) on the first and tenth day of water deficit imposition. The photosynthetic efficiency analyzes of chlorophyll a (ETRmax, Ik and α) were performed on the first and last day of water deficit imposition and 12 hours after rehydration, obtaining fast light curves through a fluorimeter. Leaf collections for biochemical analysis were performed at the beginning and end of water deficit and 12 hours after plant rehydration. Lipid peroxidation through MDA contents and antioxidant enzymatic activity SOD, APX, CAT and POD were evaluated. At the end of the experiment the leaves and roots were collected to analyze the concentrations of reducing sugars, total soluble sugars, starch, protein, proline and phenolic compounds. It was concluded that the exogenous application of the trehalose derivatives mixture proved to be efficient in mitigating damage caused by water deficit through the activation of antioxidant enzymes (SOD, APX and POD), besides promoting the accumulation of sugars, proline and phenolic compounds and improving photosynthetic efficiency in the BRS 1030 maize hybridNa agricultura um dos fatores limitantes do desenvolvimento das plantas e que afeta diretamente no resultado final da produção é a disponibilidade de água. Com as constantes mudanças climáticas, faz-se necessário a realização de estudos para a descoberta de novas tecnologias visando minimizar o estresse hídrico causado nas plantas, principalmente na fase de germinação e crescimento, floração e enchimento dos grãos. Este trabalho objetivou-se a analisar a aplicação de novos dissacarídeos derivados de trealose em plantas de milho verificando a capacidade de indução a tolerância à seca. O experimento foi conduzido em casa de vegetação, utilizando o milho híbrido BRS 1030 sensível ao déficit hídrico. O estresse hídrico foi aplicado quando as plantas atingiram o estádio vegetativo V6 e foi imposto por 12 dias, com aplicação foliar da mistura dos derivados (30mM) no primeiro e décimo dia de imposição do déficit hídrico. As análises da eficiência fotossintética da clorofila a (ETRmax, Ik e α), foram realizadas no primeiro e último dia de imposição do déficit hídrico e 12 horas após a reidratação, obtendo-se curvas rápidas de luz através de um fluorímetro. As coletas foliares para análise bioquímica foram realizadas no início e fim do déficit hídrico e 12 horas após a reidratação das plantas. Foram avaliados a peroxidação lipídica através dos teores de MDA e a atividade enzimática antioxidante SOD, APX, CAT e POD. Ao final do experimento a parte aérea e raízes foram coletadas para análises das concentrações de açúcares redutores, açúcares solúveis totais, amido, proteína, prolina e compostos fenólicos. Foi possível concluir que, a aplicação exógena da mistura dos derivados da trealose mostrou-se eficiente na mitigação de danos causados pelo déficit hídrico através da ativação das enzimas antioxidantes (SOD, APX e POD), além de promover o acúmulo de açúcares, prolina e compostos fenólicos e melhorando a eficiência fotossintética no híbrido de milho BRS 1030.application/pdfporUniversidade Federal de AlfenasPrograma de Pós-Graduação em Ciências AmbientaisUNIFAL-MGBrasilInstituto de Ciências da Naturezainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/MilhoSecasFluorescênciaClorofilaProlinaEstimulantesCIENCIAS BIOLOGICAS::BIOQUIMICADerivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídricoinfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion4542263603111139210600600893477990329266114reponame:Biblioteca Digital de Teses e Dissertações da UNIFALinstname:Universidade Federal de Alfenas (UNIFAL)instacron:UNIFALPortugal, Joice Aparecida De NovaisLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt-BR.fl_str_mv Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
title Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
spellingShingle Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
Portugal, Joice Aparecida De Novais
Milho
Secas
Fluorescência
Clorofila
Prolina
Estimulantes
CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
title_full Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
title_fullStr Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
title_full_unstemmed Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
title_sort Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
author Portugal, Joice Aparecida De Novais
author_facet Portugal, Joice Aparecida De Novais
author_role author
dc.contributor.author.fl_str_mv Portugal, Joice Aparecida De Novais
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2834667104138232
dc.contributor.advisor-co1.fl_str_mv Souza, Kamila Rezende Dázio De
dc.contributor.referee1.fl_str_mv Silva, Adriano Bortolloti Da
dc.contributor.referee2.fl_str_mv Rodrigues, Luiz Carlos Almeida
dc.contributor.advisor1.fl_str_mv Souza, Thiago Corrêa De
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2171828464199433
contributor_str_mv Souza, Kamila Rezende Dázio De
Silva, Adriano Bortolloti Da
Rodrigues, Luiz Carlos Almeida
Souza, Thiago Corrêa De
dc.subject.por.fl_str_mv Milho
Secas
Fluorescência
Clorofila
Prolina
Estimulantes
topic Milho
Secas
Fluorescência
Clorofila
Prolina
Estimulantes
CIENCIAS BIOLOGICAS::BIOQUIMICA
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOQUIMICA
description In agriculture one of the limiting factors of plant development that directly affects the final production result is water availability. With the constant climate changes, it is necessary to carry out studies to discover new technologies to minimize water stress caused on plants, especially in the germination and growth, flowering and fruit filling phase. This work aimed to analyze the application of new trehalose-derived disaccharides in maize plants by checking the drought tolerance induction capacity. The experiment was carried out in a greenhouse using BRS 1030 hybrid corn sensitive to water deficit. Water stress was applied when the plants reached vegetative stage V6 and was imposed for 12 days, with foliar application of the mixture of derivatives (30mM) on the first and tenth day of water deficit imposition. The photosynthetic efficiency analyzes of chlorophyll a (ETRmax, Ik and α) were performed on the first and last day of water deficit imposition and 12 hours after rehydration, obtaining fast light curves through a fluorimeter. Leaf collections for biochemical analysis were performed at the beginning and end of water deficit and 12 hours after plant rehydration. Lipid peroxidation through MDA contents and antioxidant enzymatic activity SOD, APX, CAT and POD were evaluated. At the end of the experiment the leaves and roots were collected to analyze the concentrations of reducing sugars, total soluble sugars, starch, protein, proline and phenolic compounds. It was concluded that the exogenous application of the trehalose derivatives mixture proved to be efficient in mitigating damage caused by water deficit through the activation of antioxidant enzymes (SOD, APX and POD), besides promoting the accumulation of sugars, proline and phenolic compounds and improving photosynthetic efficiency in the BRS 1030 maize hybrid
publishDate 2019
dc.date.issued.fl_str_mv 2019-12-20
dc.date.accessioned.fl_str_mv 2021-06-15T12:37:28Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv PORTUGAL, Joice Aparecida de Novais. Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico. 2019. 55 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Alfenas, Alfenas, MG, 2019.
dc.identifier.uri.fl_str_mv https://repositorio.unifal-mg.edu.br/handle/123456789/1809
identifier_str_mv PORTUGAL, Joice Aparecida de Novais. Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico. 2019. 55 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Alfenas, Alfenas, MG, 2019.
url https://repositorio.unifal-mg.edu.br/handle/123456789/1809
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Ambientais
dc.publisher.initials.fl_str_mv UNIFAL-MG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Ciências da Natureza
publisher.none.fl_str_mv Universidade Federal de Alfenas
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