Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico
| Ano de defesa: | 2019 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | , |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal de Alfenas
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Ambientais
|
| Departamento: |
Instituto de Ciências da Natureza
|
| País: |
Brasil
|
| Palavras-chave em Português: | |
| Área do conhecimento CNPq: | |
| Link de acesso: | https://repositorio.unifal-mg.edu.br/handle/123456789/1809 |
Resumo: | In agriculture one of the limiting factors of plant development that directly affects the final production result is water availability. With the constant climate changes, it is necessary to carry out studies to discover new technologies to minimize water stress caused on plants, especially in the germination and growth, flowering and fruit filling phase. This work aimed to analyze the application of new trehalose-derived disaccharides in maize plants by checking the drought tolerance induction capacity. The experiment was carried out in a greenhouse using BRS 1030 hybrid corn sensitive to water deficit. Water stress was applied when the plants reached vegetative stage V6 and was imposed for 12 days, with foliar application of the mixture of derivatives (30mM) on the first and tenth day of water deficit imposition. The photosynthetic efficiency analyzes of chlorophyll a (ETRmax, Ik and α) were performed on the first and last day of water deficit imposition and 12 hours after rehydration, obtaining fast light curves through a fluorimeter. Leaf collections for biochemical analysis were performed at the beginning and end of water deficit and 12 hours after plant rehydration. Lipid peroxidation through MDA contents and antioxidant enzymatic activity SOD, APX, CAT and POD were evaluated. At the end of the experiment the leaves and roots were collected to analyze the concentrations of reducing sugars, total soluble sugars, starch, protein, proline and phenolic compounds. It was concluded that the exogenous application of the trehalose derivatives mixture proved to be efficient in mitigating damage caused by water deficit through the activation of antioxidant enzymes (SOD, APX and POD), besides promoting the accumulation of sugars, proline and phenolic compounds and improving photosynthetic efficiency in the BRS 1030 maize hybrid |
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Portugal, Joice Aparecida De Novaishttp://lattes.cnpq.br/2834667104138232Souza, Kamila Rezende Dázio DeSilva, Adriano Bortolloti DaRodrigues, Luiz Carlos AlmeidaSouza, Thiago Corrêa Dehttp://lattes.cnpq.br/21718284641994332021-06-15T12:37:28Z2019-12-20PORTUGAL, Joice Aparecida de Novais. Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico. 2019. 55 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Alfenas, Alfenas, MG, 2019.https://repositorio.unifal-mg.edu.br/handle/123456789/1809In agriculture one of the limiting factors of plant development that directly affects the final production result is water availability. With the constant climate changes, it is necessary to carry out studies to discover new technologies to minimize water stress caused on plants, especially in the germination and growth, flowering and fruit filling phase. This work aimed to analyze the application of new trehalose-derived disaccharides in maize plants by checking the drought tolerance induction capacity. The experiment was carried out in a greenhouse using BRS 1030 hybrid corn sensitive to water deficit. Water stress was applied when the plants reached vegetative stage V6 and was imposed for 12 days, with foliar application of the mixture of derivatives (30mM) on the first and tenth day of water deficit imposition. The photosynthetic efficiency analyzes of chlorophyll a (ETRmax, Ik and α) were performed on the first and last day of water deficit imposition and 12 hours after rehydration, obtaining fast light curves through a fluorimeter. Leaf collections for biochemical analysis were performed at the beginning and end of water deficit and 12 hours after plant rehydration. Lipid peroxidation through MDA contents and antioxidant enzymatic activity SOD, APX, CAT and POD were evaluated. At the end of the experiment the leaves and roots were collected to analyze the concentrations of reducing sugars, total soluble sugars, starch, protein, proline and phenolic compounds. It was concluded that the exogenous application of the trehalose derivatives mixture proved to be efficient in mitigating damage caused by water deficit through the activation of antioxidant enzymes (SOD, APX and POD), besides promoting the accumulation of sugars, proline and phenolic compounds and improving photosynthetic efficiency in the BRS 1030 maize hybridNa agricultura um dos fatores limitantes do desenvolvimento das plantas e que afeta diretamente no resultado final da produção é a disponibilidade de água. Com as constantes mudanças climáticas, faz-se necessário a realização de estudos para a descoberta de novas tecnologias visando minimizar o estresse hídrico causado nas plantas, principalmente na fase de germinação e crescimento, floração e enchimento dos grãos. Este trabalho objetivou-se a analisar a aplicação de novos dissacarídeos derivados de trealose em plantas de milho verificando a capacidade de indução a tolerância à seca. O experimento foi conduzido em casa de vegetação, utilizando o milho híbrido BRS 1030 sensível ao déficit hídrico. O estresse hídrico foi aplicado quando as plantas atingiram o estádio vegetativo V6 e foi imposto por 12 dias, com aplicação foliar da mistura dos derivados (30mM) no primeiro e décimo dia de imposição do déficit hídrico. As análises da eficiência fotossintética da clorofila a (ETRmax, Ik e α), foram realizadas no primeiro e último dia de imposição do déficit hídrico e 12 horas após a reidratação, obtendo-se curvas rápidas de luz através de um fluorímetro. As coletas foliares para análise bioquímica foram realizadas no início e fim do déficit hídrico e 12 horas após a reidratação das plantas. Foram avaliados a peroxidação lipídica através dos teores de MDA e a atividade enzimática antioxidante SOD, APX, CAT e POD. Ao final do experimento a parte aérea e raízes foram coletadas para análises das concentrações de açúcares redutores, açúcares solúveis totais, amido, proteína, prolina e compostos fenólicos. Foi possível concluir que, a aplicação exógena da mistura dos derivados da trealose mostrou-se eficiente na mitigação de danos causados pelo déficit hídrico através da ativação das enzimas antioxidantes (SOD, APX e POD), além de promover o acúmulo de açúcares, prolina e compostos fenólicos e melhorando a eficiência fotossintética no híbrido de milho BRS 1030.application/pdfporUniversidade Federal de AlfenasPrograma de Pós-Graduação em Ciências AmbientaisUNIFAL-MGBrasilInstituto de Ciências da Naturezainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/MilhoSecasFluorescênciaClorofilaProlinaEstimulantesCIENCIAS BIOLOGICAS::BIOQUIMICADerivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídricoinfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion4542263603111139210600600893477990329266114reponame:Biblioteca Digital de Teses e Dissertações da UNIFALinstname:Universidade Federal de Alfenas (UNIFAL)instacron:UNIFALPortugal, Joice Aparecida De NovaisLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt-BR.fl_str_mv |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico |
| title |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico |
| spellingShingle |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico Portugal, Joice Aparecida De Novais Milho Secas Fluorescência Clorofila Prolina Estimulantes CIENCIAS BIOLOGICAS::BIOQUIMICA |
| title_short |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico |
| title_full |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico |
| title_fullStr |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico |
| title_full_unstemmed |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico |
| title_sort |
Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico |
| author |
Portugal, Joice Aparecida De Novais |
| author_facet |
Portugal, Joice Aparecida De Novais |
| author_role |
author |
| dc.contributor.author.fl_str_mv |
Portugal, Joice Aparecida De Novais |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2834667104138232 |
| dc.contributor.advisor-co1.fl_str_mv |
Souza, Kamila Rezende Dázio De |
| dc.contributor.referee1.fl_str_mv |
Silva, Adriano Bortolloti Da |
| dc.contributor.referee2.fl_str_mv |
Rodrigues, Luiz Carlos Almeida |
| dc.contributor.advisor1.fl_str_mv |
Souza, Thiago Corrêa De |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2171828464199433 |
| contributor_str_mv |
Souza, Kamila Rezende Dázio De Silva, Adriano Bortolloti Da Rodrigues, Luiz Carlos Almeida Souza, Thiago Corrêa De |
| dc.subject.por.fl_str_mv |
Milho Secas Fluorescência Clorofila Prolina Estimulantes |
| topic |
Milho Secas Fluorescência Clorofila Prolina Estimulantes CIENCIAS BIOLOGICAS::BIOQUIMICA |
| dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOQUIMICA |
| description |
In agriculture one of the limiting factors of plant development that directly affects the final production result is water availability. With the constant climate changes, it is necessary to carry out studies to discover new technologies to minimize water stress caused on plants, especially in the germination and growth, flowering and fruit filling phase. This work aimed to analyze the application of new trehalose-derived disaccharides in maize plants by checking the drought tolerance induction capacity. The experiment was carried out in a greenhouse using BRS 1030 hybrid corn sensitive to water deficit. Water stress was applied when the plants reached vegetative stage V6 and was imposed for 12 days, with foliar application of the mixture of derivatives (30mM) on the first and tenth day of water deficit imposition. The photosynthetic efficiency analyzes of chlorophyll a (ETRmax, Ik and α) were performed on the first and last day of water deficit imposition and 12 hours after rehydration, obtaining fast light curves through a fluorimeter. Leaf collections for biochemical analysis were performed at the beginning and end of water deficit and 12 hours after plant rehydration. Lipid peroxidation through MDA contents and antioxidant enzymatic activity SOD, APX, CAT and POD were evaluated. At the end of the experiment the leaves and roots were collected to analyze the concentrations of reducing sugars, total soluble sugars, starch, protein, proline and phenolic compounds. It was concluded that the exogenous application of the trehalose derivatives mixture proved to be efficient in mitigating damage caused by water deficit through the activation of antioxidant enzymes (SOD, APX and POD), besides promoting the accumulation of sugars, proline and phenolic compounds and improving photosynthetic efficiency in the BRS 1030 maize hybrid |
| publishDate |
2019 |
| dc.date.issued.fl_str_mv |
2019-12-20 |
| dc.date.accessioned.fl_str_mv |
2021-06-15T12:37:28Z |
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info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/publishedVersion |
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masterThesis |
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PORTUGAL, Joice Aparecida de Novais. Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico. 2019. 55 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Alfenas, Alfenas, MG, 2019. |
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https://repositorio.unifal-mg.edu.br/handle/123456789/1809 |
| identifier_str_mv |
PORTUGAL, Joice Aparecida de Novais. Derivados de trealose no sistema antioxidante e fotossintético de plantas de milho expostas ao déficit hídrico. 2019. 55 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Alfenas, Alfenas, MG, 2019. |
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UNIFAL-MG |
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Brasil |
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Instituto de Ciências da Natureza |
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Universidade Federal de Alfenas |
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