Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages

Detalhes bibliográficos
Ano de defesa: 2023
Autor(a) principal: Rosa, Caio Pupin lattes
Orientador(a): Almeida, Leonardo Augusto De lattes
Banca de defesa: Tana, Fernanda De Lima, Peloso, Eduardo De Figueiredo
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Alfenas
Programa de Pós-Graduação: Programa de Pós-graduação em Ciências Biológicas
Departamento: Instituto de Ciências da Natureza
País: Brasil
Palavras-chave em Português:
Imunidade inata treinada
Macrófagos
BCG
Pseudomonas aeruginosa
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS
Link de acesso: https://repositorio.unifal-mg.edu.br/handle/123456789/2198
Resumo: Innate trained immunity is a recent concept regarding the training or tolerance acquired by macrophages, natural killer (NK) cells, dendritic cells and epithelial cells before heterologous stimulus. Given the novel data concerning macrophage training in pneumonia models and, thereby, the possibility of prevention and/or treatment of respiratory infections, this work aims to evaluate macrophage priming in vitro by BCG and/or HKPa. For this purpose, C57BL/6 mice bone-marrow-derived macrophages were plated and stimulated with BCG and/or HKPa. CD11b+ macrophage displayed activation through higher immunofluorescence of CD80/86, when homologous or heterologous stimulation occurred with BCG and/or HKPa. These cells also showed pro-oxidant status before BCG stimulus, through TBARS, TRAP and NADPH-oxidase tests. Nitric oxide concentration was augmented in BCG heterologous stimulation. Moreover, there was higher secretion of IL-6 when BCG heterologous stimulation occurred. However, homologous HKPa stimulation augmented IL-10 expression and secretion levels. Therefore, this study suggests that macrophages were primed by BCG, when there was heterologous stimulation with HKPa, and that homologous stimulation with HKPa induced immune tolerance.
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spelling Rosa, Caio Pupinhttp://lattes.cnpq.br/5381507919608022Tana, Fernanda De LimaPeloso, Eduardo De FigueiredoAlmeida, Leonardo Augusto Dehttp://lattes.cnpq.br/32414379375742852023-03-24T11:59:06Z2023-02-06ROSA, Caio Pupin. Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages. 2023. 36 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Alfenas, Alfenas, MG, 2023.https://repositorio.unifal-mg.edu.br/handle/123456789/2198Innate trained immunity is a recent concept regarding the training or tolerance acquired by macrophages, natural killer (NK) cells, dendritic cells and epithelial cells before heterologous stimulus. Given the novel data concerning macrophage training in pneumonia models and, thereby, the possibility of prevention and/or treatment of respiratory infections, this work aims to evaluate macrophage priming in vitro by BCG and/or HKPa. For this purpose, C57BL/6 mice bone-marrow-derived macrophages were plated and stimulated with BCG and/or HKPa. CD11b+ macrophage displayed activation through higher immunofluorescence of CD80/86, when homologous or heterologous stimulation occurred with BCG and/or HKPa. These cells also showed pro-oxidant status before BCG stimulus, through TBARS, TRAP and NADPH-oxidase tests. Nitric oxide concentration was augmented in BCG heterologous stimulation. Moreover, there was higher secretion of IL-6 when BCG heterologous stimulation occurred. However, homologous HKPa stimulation augmented IL-10 expression and secretion levels. Therefore, this study suggests that macrophages were primed by BCG, when there was heterologous stimulation with HKPa, and that homologous stimulation with HKPa induced immune tolerance.A imunidade inata treinada é conceito recente que diz respeito ao treinamento de macrófagos, células natural killer (NK), células dendríticas e células epiteliais frente à estimulação heteróloga, levando ao treinamento ou à tolerância de resposta. Considerando os recentes indícios de treinamento em modelos de estudo de pneumonias e as possíveis implicações na prevenção e no tratamento dessas afecções, o presente trabalho se propôs a avaliar o perfil de resposta de macrófagos estimulados previamente pelo Bacilo de Calmatte-Guérin (BCG) e/ou Pseudomonas aeruginosa morta pelo calor (HKPa) seguido de estímulos homólogos ou heterólogos. Para isso, macrófagos derivados da medula óssea de camundongos C57BL/6 foram obtidos e estimulados com BCG e/ou HKPa. Houve ativação de macrófagos CD11b+ pelo aumento da imunofluorescência emitida por CD80/86, quando de estimulação homóloga ou heteróloga com BCG e/ou HKPa. As mesmas células demonstraram tendência pró- oxidante pelos testes da NADPH-oxidase, TBARS e TRAP, quando do estímulo com BCG e aumento da secreção de óxido nítrico quando da estimulação heteróloga iniciada com BCG. Ainda, houve aumento da secreção de IL-6 quando da estimulação heteróloga iniciada por BCG. Contudo, a estimulação homóloga com HKPa aumentou tanto a expressão quanto a secreção de IL-10. Portanto, o presente trabalho sugere o priming de macrófagos pelo BCG, quando da estimulação heteróloga com HKPa, e sugere tolerância induzida pela estimulação homóloga de HKPa.application/pdfporUniversidade Federal de AlfenasPrograma de Pós-graduação em Ciências BiológicasUNIFAL-MGBrasilInstituto de Ciências da Naturezainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Imunidade inata treinadaMacrófagosBCGPseudomonas aeruginosaCIENCIAS BIOLOGICASHeat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophagesinfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion4542263603111139210600600-3439178843068202161reponame:Repositório Institucional da Universidade Federal de Alfenas - RiUnifalinstname:Universidade Federal de Alfenas (UNIFAL)instacron:UNIFALRosa, Caio PupinLICENSElicense.txtlicense.txttext/plain; charset=utf-81987https://repositorio.unifal-mg.edu.br/bitstreams/e43f0f50-e76a-4338-9b20-3fa5e41db1bc/download31555718c4fc75849dd08f27935d4f6bMD51CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.pt-BR.fl_str_mv Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
title Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
spellingShingle Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
Rosa, Caio Pupin
Imunidade inata treinada
Macrófagos
BCG
Pseudomonas aeruginosa
CIENCIAS BIOLOGICAS
title_short Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
title_full Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
title_fullStr Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
title_full_unstemmed Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
title_sort Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages
author Rosa, Caio Pupin
author_facet Rosa, Caio Pupin
author_role author
dc.contributor.author.fl_str_mv Rosa, Caio Pupin
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5381507919608022
dc.contributor.referee1.fl_str_mv Tana, Fernanda De Lima
dc.contributor.referee2.fl_str_mv Peloso, Eduardo De Figueiredo
dc.contributor.advisor1.fl_str_mv Almeida, Leonardo Augusto De
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3241437937574285
contributor_str_mv Tana, Fernanda De Lima
Peloso, Eduardo De Figueiredo
Almeida, Leonardo Augusto De
dc.subject.por.fl_str_mv Imunidade inata treinada
Macrófagos
BCG
Pseudomonas aeruginosa
topic Imunidade inata treinada
Macrófagos
BCG
Pseudomonas aeruginosa
CIENCIAS BIOLOGICAS
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS
description Innate trained immunity is a recent concept regarding the training or tolerance acquired by macrophages, natural killer (NK) cells, dendritic cells and epithelial cells before heterologous stimulus. Given the novel data concerning macrophage training in pneumonia models and, thereby, the possibility of prevention and/or treatment of respiratory infections, this work aims to evaluate macrophage priming in vitro by BCG and/or HKPa. For this purpose, C57BL/6 mice bone-marrow-derived macrophages were plated and stimulated with BCG and/or HKPa. CD11b+ macrophage displayed activation through higher immunofluorescence of CD80/86, when homologous or heterologous stimulation occurred with BCG and/or HKPa. These cells also showed pro-oxidant status before BCG stimulus, through TBARS, TRAP and NADPH-oxidase tests. Nitric oxide concentration was augmented in BCG heterologous stimulation. Moreover, there was higher secretion of IL-6 when BCG heterologous stimulation occurred. However, homologous HKPa stimulation augmented IL-10 expression and secretion levels. Therefore, this study suggests that macrophages were primed by BCG, when there was heterologous stimulation with HKPa, and that homologous stimulation with HKPa induced immune tolerance.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-03-24T11:59:06Z
dc.date.issued.fl_str_mv 2023-02-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv ROSA, Caio Pupin. Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages. 2023. 36 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Alfenas, Alfenas, MG, 2023.
dc.identifier.uri.fl_str_mv https://repositorio.unifal-mg.edu.br/handle/123456789/2198
identifier_str_mv ROSA, Caio Pupin. Heat-killed Pseudomonas aeruginosa drives M1 polarization on BCG- primed macrophages. 2023. 36 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Alfenas, Alfenas, MG, 2023.
url https://repositorio.unifal-mg.edu.br/handle/123456789/2198
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dc.relation.confidence.fl_str_mv 600
600
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dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ciências Biológicas
dc.publisher.initials.fl_str_mv UNIFAL-MG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Ciências da Natureza
publisher.none.fl_str_mv Universidade Federal de Alfenas
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