AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS

Detalhes bibliográficos
Ano de defesa: 2022
Autor(a) principal: Camargo, Marilu Chaves Gomes Dresch lattes
Orientador(a): Gandra, Rinaldo Ferreira
Banca de defesa: Conegero, Andréia Sanches, Lordani, Tarcísio Vítor Augusto
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual do Oeste do Paraná
Cascavel
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas
Departamento: Centro de Ciências Médicas e Farmacêuticas
País: Brasil
Palavras-chave em Português:
Atividade antifúngica
Cryptococcus neoformans
Levedura killer
Toxina killer
Killer yeast
Killer toxin
Palavras-chave em Inglês:
Antifungal activity
Cryptococcus neoformans
Área do conhecimento CNPq:
CIÊNCIAS FARMACÊUTICAS
Link de acesso: https://tede.unioeste.br/handle/tede/6623
Resumo: Cryptococcus neoformans is an opportunistic yeast that can lodge in the central nervous system causing meningitis, meningoencephalitis and encephalitis, mainly in individuals with some compromised immune system; being responsible for 4.5% of opportunistic infections that affect immunosuppressed patients. The use of antifungals can lead to resistance and toxicity. The mycocins produced by Wickerhamomyces anomalus capable of inhibiting the growth of eukaryotic and prokaryotic microorganisms. The objective of this work is to evaluate the susceptibility of Cryptococcus neoformans strains against mycocins produced by Wickerhamomyces anomalus. The results showed, by the broth microdilution test, that 100% of the Cryptococcus neoformans strains tested were inhibited when the culture supernatant containing mycocins was used. In the proteinase production test, 50% of the strains tested were strongly proteinase producers. All strains that had their enzymatic activity strongly positive were inhibited when tested in culture medium containing a sub-inhibitory concentration of β-glucanases. Therefore, we can say that mycocins have a vast pharmacological potential.
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spelling Gandra, Rinaldo FerreiraConegero, Andréia SanchesLordani, Tarcísio Vítor Augustohttp://lattes.cnpq.br/0784838642972820Camargo, Marilu Chaves Gomes Dresch2023-05-09T12:17:20Z2022-11-30Camargo, Marilu Chaves Gomes Dresch. AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS. 2022. 36 f. Dissertação( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel.https://tede.unioeste.br/handle/tede/6623Cryptococcus neoformans is an opportunistic yeast that can lodge in the central nervous system causing meningitis, meningoencephalitis and encephalitis, mainly in individuals with some compromised immune system; being responsible for 4.5% of opportunistic infections that affect immunosuppressed patients. The use of antifungals can lead to resistance and toxicity. The mycocins produced by Wickerhamomyces anomalus capable of inhibiting the growth of eukaryotic and prokaryotic microorganisms. The objective of this work is to evaluate the susceptibility of Cryptococcus neoformans strains against mycocins produced by Wickerhamomyces anomalus. The results showed, by the broth microdilution test, that 100% of the Cryptococcus neoformans strains tested were inhibited when the culture supernatant containing mycocins was used. In the proteinase production test, 50% of the strains tested were strongly proteinase producers. All strains that had their enzymatic activity strongly positive were inhibited when tested in culture medium containing a sub-inhibitory concentration of β-glucanases. Therefore, we can say that mycocins have a vast pharmacological potential.O Cryptococcus neoformans é uma levedura oportunista que pode se alojar no sistema nervoso central causando meningite, meningoencefalite e encefalite, principalmente em indivíduos com algum comprometimento do sistema imune; sendo responsável por 4,5 % das infecções oportunistas que acometem pacientes imunodeprimidos. O uso de antifúngicos pode levar a resistência e toxicidade. As micocinas produzidas por Wickerhamomyces anomalus são capazes de inibir o crescimento de microrganismos eucariotos e procariotos. O objetivo deste trabalho é avaliar a susceptibilidade das cepas de Cryptococcus neoformans, frente às micocinas produzidas por Wickerhamomyces anomalus. Os resultados mostraram, pelo teste de microdiluição em caldo, que 100% das cepas de Cryptococcus neoformans testadas foram inibidas quando utilizado o sobrenadante de cultura contendo micocinas. No teste de produção de proteinases, 50% das cepas testadas foram fortemente produtoras de proteinases. Todas as cepas que tiveram a sua atividade enzimática fortemente positivas foram inibidas quando testadas em meio de cultura contendo uma concentração subinibitória de β-glucanases. Diante disso, podemos afirmar que as micocinas possuem um potencial farmacológico vastoSubmitted by Edineia Teixeira (edineia.teixeira@unioeste.br) on 2023-05-09T12:17:19Z No. of bitstreams: 2 MARILU_CAMARGO.2022.pdf: 789518 bytes, checksum: 9a3d9525a7ca28861450282863bd788b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2023-05-09T12:17:20Z (GMT). No. of bitstreams: 2 MARILU_CAMARGO.2022.pdf: 789518 bytes, checksum: 9a3d9525a7ca28861450282863bd788b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2022-11-30application/pdfpor6588633818200016417500Universidade Estadual do Oeste do ParanáCascavelPrograma de Pós-Graduação em Ciências FarmacêuticasUNIOESTEBrasilCentro de Ciências Médicas e Farmacêuticashttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessAtividade antifúngicaCryptococcus neoformansLevedura killerToxina killerKiller yeastKiller toxinAntifungal activityCryptococcus neoformansCIÊNCIAS FARMACÊUTICASAÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAISACTION OF MYCOKINES PRODUCED BY Wickerhamomyces anomalus ON ENVIRONMENTAL ISOLATES OF Cryptococcus neoformansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7878055067573953101600600-8940439713387849267reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALMARILU_CAMARGO.2022.pdfMARILU_CAMARGO.2022.pdfapplication/pdf789518http://tede.unioeste.br:8080/tede/bitstream/tede/6623/5/MARILU_CAMARGO.2022.pdf9a3d9525a7ca28861450282863bd788bMD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
dc.title.alternative.eng.fl_str_mv ACTION OF MYCOKINES PRODUCED BY Wickerhamomyces anomalus ON ENVIRONMENTAL ISOLATES OF Cryptococcus neoformans
title AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
spellingShingle AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
Camargo, Marilu Chaves Gomes Dresch
Atividade antifúngica
Cryptococcus neoformans
Levedura killer
Toxina killer
Killer yeast
Killer toxin
Antifungal activity
Cryptococcus neoformans
CIÊNCIAS FARMACÊUTICAS
title_short AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
title_full AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
title_fullStr AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
title_full_unstemmed AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
title_sort AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS
author Camargo, Marilu Chaves Gomes Dresch
author_facet Camargo, Marilu Chaves Gomes Dresch
author_role author
dc.contributor.advisor1.fl_str_mv Gandra, Rinaldo Ferreira
dc.contributor.referee1.fl_str_mv Conegero, Andréia Sanches
dc.contributor.referee2.fl_str_mv Lordani, Tarcísio Vítor Augusto
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0784838642972820
dc.contributor.author.fl_str_mv Camargo, Marilu Chaves Gomes Dresch
contributor_str_mv Gandra, Rinaldo Ferreira
Conegero, Andréia Sanches
Lordani, Tarcísio Vítor Augusto
dc.subject.por.fl_str_mv Atividade antifúngica
Cryptococcus neoformans
Levedura killer
Toxina killer
Killer yeast
Killer toxin
topic Atividade antifúngica
Cryptococcus neoformans
Levedura killer
Toxina killer
Killer yeast
Killer toxin
Antifungal activity
Cryptococcus neoformans
CIÊNCIAS FARMACÊUTICAS
dc.subject.eng.fl_str_mv Antifungal activity
Cryptococcus neoformans
dc.subject.cnpq.fl_str_mv CIÊNCIAS FARMACÊUTICAS
description Cryptococcus neoformans is an opportunistic yeast that can lodge in the central nervous system causing meningitis, meningoencephalitis and encephalitis, mainly in individuals with some compromised immune system; being responsible for 4.5% of opportunistic infections that affect immunosuppressed patients. The use of antifungals can lead to resistance and toxicity. The mycocins produced by Wickerhamomyces anomalus capable of inhibiting the growth of eukaryotic and prokaryotic microorganisms. The objective of this work is to evaluate the susceptibility of Cryptococcus neoformans strains against mycocins produced by Wickerhamomyces anomalus. The results showed, by the broth microdilution test, that 100% of the Cryptococcus neoformans strains tested were inhibited when the culture supernatant containing mycocins was used. In the proteinase production test, 50% of the strains tested were strongly proteinase producers. All strains that had their enzymatic activity strongly positive were inhibited when tested in culture medium containing a sub-inhibitory concentration of β-glucanases. Therefore, we can say that mycocins have a vast pharmacological potential.
publishDate 2022
dc.date.issued.fl_str_mv 2022-11-30
dc.date.accessioned.fl_str_mv 2023-05-09T12:17:20Z
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dc.identifier.citation.fl_str_mv Camargo, Marilu Chaves Gomes Dresch. AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS. 2022. 36 f. Dissertação( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel.
dc.identifier.uri.fl_str_mv https://tede.unioeste.br/handle/tede/6623
identifier_str_mv Camargo, Marilu Chaves Gomes Dresch. AÇÃO DE MICOCINAS PRODUZIDAS POR Wickerhamomyces anomalus SOBRE ISOLADOS de Cryptococcus neoformans AMBIENTAIS. 2022. 36 f. Dissertação( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel.
url https://tede.unioeste.br/handle/tede/6623
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language por
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dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Cascavel
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Farmacêuticas
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Centro de Ciências Médicas e Farmacêuticas
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Cascavel
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