Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Savoldi, Tárcio Enrico lattes
Orientador(a): Fiorese, Mônica Lady lattes
Banca de defesa: Fariña, Luciana Oliveira de lattes, Scheufele, Fabiano Bisinella lattes, Fiorese, Mônica Lady lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual do Oeste do Paraná
Toledo
Programa de Pós-Graduação: Programa de Pós-Graduação em Engenharia Química
Departamento: Centro de Engenharias e Ciências Exatas
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://tede.unioeste.br/handle/tede/6074
Resumo: Probiotics are a class of microorganisms that can provide benefits to those who consume them. The yeast Saccharomyces boulardii has its highlight to the ability to fight gastrointestinal infections of various kinds. In order to increase the resistance of these microorganisms various microencapsulation techniques have been optimized over the years. This techniques basically consists in coating particles with a material which will provide him protection. The spray-drying technique is one methods that can be employed in microencapsulation. The aim of this study was to develop microcapsules with the probiotic yeast Saccharomyces boulardii using different proteins and their combination with maltodextrin as wall materials. First, they were studied 3 different broths for S. boulardii in order to select the one that provides greater amount of viable cells. Subsequently the cells were microencapsulated in 4 different types of proteins, as well the maltodextrin combinations. This process demonstrated that, among the proteins used, rice protein was the one that provided the highest survival rate of S. boulardii of 98%. The influence of drying temperature, percentage of maltodextrin and feed rate on the survival of S. boulardii were investigated from a factorial 23 experimental design. Tests performed after microencapsulation showed that microcapsules remained stable by At least 15 days after microencapsulation, FTIR analyzes show that there is little interaction between yeast cells and encapsulating agents and thermogravimetric analysis showed that microcapsules are quite resistant to heat. Showing that microencapsulation by spray drying of S. boulardii with different kinds of proteins mixed with maltodextrin is a good method.
id UNIOESTE-1_4782f11db3660b93a1bf1ce4c65e8bbd
oai_identifier_str oai:tede.unioeste.br:tede/6074
network_acronym_str UNIOESTE-1
network_name_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
repository_id_str
spelling Fiorese, Mônica Ladyhttp://lattes.cnpq.br/5160443525915040Drunkler, Deisy Alessandrahttp://lattes.cnpq.br/1887851918491484Fariña, Luciana Oliveira dehttp://lattes.cnpq.br/2043990245681647Scheufele, Fabiano Bisinellahttp://lattes.cnpq.br/4578180806056815Fiorese, Mônica Ladyhttp://lattes.cnpq.br/5160443525915040http://lattes.cnpq.br/6877993218801186Savoldi, Tárcio Enrico2022-06-07T17:39:27Z2017-03-10SAVOLDI, Tárcio Enrico. Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina. 2017. 81 f. Dissertação (Mestrado em Engenharia Química) - Universidade Estadual do Oeste do Paraná, Toledo, 2017.https://tede.unioeste.br/handle/tede/6074Probiotics are a class of microorganisms that can provide benefits to those who consume them. The yeast Saccharomyces boulardii has its highlight to the ability to fight gastrointestinal infections of various kinds. In order to increase the resistance of these microorganisms various microencapsulation techniques have been optimized over the years. This techniques basically consists in coating particles with a material which will provide him protection. The spray-drying technique is one methods that can be employed in microencapsulation. The aim of this study was to develop microcapsules with the probiotic yeast Saccharomyces boulardii using different proteins and their combination with maltodextrin as wall materials. First, they were studied 3 different broths for S. boulardii in order to select the one that provides greater amount of viable cells. Subsequently the cells were microencapsulated in 4 different types of proteins, as well the maltodextrin combinations. This process demonstrated that, among the proteins used, rice protein was the one that provided the highest survival rate of S. boulardii of 98%. The influence of drying temperature, percentage of maltodextrin and feed rate on the survival of S. boulardii were investigated from a factorial 23 experimental design. Tests performed after microencapsulation showed that microcapsules remained stable by At least 15 days after microencapsulation, FTIR analyzes show that there is little interaction between yeast cells and encapsulating agents and thermogravimetric analysis showed that microcapsules are quite resistant to heat. Showing that microencapsulation by spray drying of S. boulardii with different kinds of proteins mixed with maltodextrin is a good method.Microrganismos probióticos são uma classe de microrganismos que podem prover benefícios a quem consumi-los. Nesse meio a levedura Saccharomyces boulardii tem seu destaque por ter capacidade de combater infecções gastrointestinais de diversos tipos. Objetivando viabilizar o aumento da resistência desses microrganismos várias técnicas de microencapsulação vem sido otimizadas ao longo dos anos. Esta técnica consiste, basicamente, no revestimento de partículas por um material que lhe fornecerá proteção. A técnica de spray-drying é um dos métodos que pode ser empregado na microencapsulação. O objetivo deste estudo foi desenvolver microcápsulas com a levedura probiótica Saccharomyces boulardii utilizando como agentes carreadores diferentes proteínas e suas combinações com maltodextrina como materiais de parede. Primeiramente foram avaliados diferentes concentrações iniciais de glicose e a aeração nos meios de cultivos de S. boulardii afim de selecionar aquele que fornecesse maior quantidade de células viáveis. Posteriormente as células foram microencapsuladas em 4 tipos diferentes de proteínas assim como as suas combinações com maltodextrina. Este processo demonstrou que, dentre as proteínas utilizadas, a proteína de arroz foi a que propiciou o maior índice de sobrevivência de S. boulardii de 68,88%. Na posse desses dados, a influência da Temperatura de secagem, porcentagem de maltodextrina e vazão de alimentação na sobrevivência da S. boulardii foi investigada a partir de um planejamento experimental fatorial 23. Os testes realizados após a microencapsulação mostram que as microcápsulas permanecem estáveis por pelo menos 15 dias após a microencapsulação, as análises de FTIR, mostram que há pouca alteração encapsulantes após a secagem e a análise termogravimétrica mostrou que as microcápsulas são bastante resistentes ao calor. Evidenciando assim a aplicabilidade deste método na microencapsulação da levedura S. boulardii com diferentes proteínas e maltodextrina.Submitted by Marilene Donadel (marilene.donadel@unioeste.br) on 2022-06-07T17:39:27Z No. of bitstreams: 1 Tarcio_Savoldi_2017.pdf: 2290125 bytes, checksum: 5c29e577754b06f1835c92dca33e0f2c (MD5)Made available in DSpace on 2022-06-07T17:39:27Z (GMT). No. of bitstreams: 1 Tarcio_Savoldi_2017.pdf: 2290125 bytes, checksum: 5c29e577754b06f1835c92dca33e0f2c (MD5) Previous issue date: 2017-03-10Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfpor-2624803687637593200500Universidade Estadual do Oeste do ParanáToledoPrograma de Pós-Graduação em Engenharia QuímicaUNIOESTEBrasilCentro de Engenharias e Ciências ExatasProbióticosMateriais de paredeProteína vegetalSaccharomyces boulardiiMicroencapsulationSpray-dryerENGENHARIAS::ENGENHARIA QUIMICAMicroencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrinaMicroencapsulation of Saccharomyces boulardii by spray-drying with different proteins combined with maltodextrininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1582274381427649589600600600600-7734402124082146922-18486402610968708782075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALTarcio_Savoldi_2017.pdfTarcio_Savoldi_2017.pdfapplication/pdf2290125http://tede.unioeste.br:8080/tede/bitstream/tede/6074/2/Tarcio_Savoldi_2017.pdf5c29e577754b06f1835c92dca33e0f2cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.unioeste.br:8080/tede/bitstream/tede/6074/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/60742022-06-07 14:39:27.365oai:tede.unioeste.br:tede/6074Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2022-06-07T17:39:27Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
dc.title.alternative.eng.fl_str_mv Microencapsulation of Saccharomyces boulardii by spray-drying with different proteins combined with maltodextrin
title Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
spellingShingle Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
Savoldi, Tárcio Enrico
Probióticos
Materiais de parede
Proteína vegetal
Saccharomyces boulardii
Microencapsulation
Spray-dryer
ENGENHARIAS::ENGENHARIA QUIMICA
title_short Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
title_full Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
title_fullStr Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
title_full_unstemmed Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
title_sort Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina
author Savoldi, Tárcio Enrico
author_facet Savoldi, Tárcio Enrico
author_role author
dc.contributor.advisor1.fl_str_mv Fiorese, Mônica Lady
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5160443525915040
dc.contributor.advisor-co1.fl_str_mv Drunkler, Deisy Alessandra
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1887851918491484
dc.contributor.referee1.fl_str_mv Fariña, Luciana Oliveira de
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/2043990245681647
dc.contributor.referee2.fl_str_mv Scheufele, Fabiano Bisinella
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/4578180806056815
dc.contributor.referee3.fl_str_mv Fiorese, Mônica Lady
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/5160443525915040
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6877993218801186
dc.contributor.author.fl_str_mv Savoldi, Tárcio Enrico
contributor_str_mv Fiorese, Mônica Lady
Drunkler, Deisy Alessandra
Fariña, Luciana Oliveira de
Scheufele, Fabiano Bisinella
Fiorese, Mônica Lady
dc.subject.por.fl_str_mv Probióticos
Materiais de parede
Proteína vegetal
topic Probióticos
Materiais de parede
Proteína vegetal
Saccharomyces boulardii
Microencapsulation
Spray-dryer
ENGENHARIAS::ENGENHARIA QUIMICA
dc.subject.eng.fl_str_mv Saccharomyces boulardii
Microencapsulation
Spray-dryer
dc.subject.cnpq.fl_str_mv ENGENHARIAS::ENGENHARIA QUIMICA
description Probiotics are a class of microorganisms that can provide benefits to those who consume them. The yeast Saccharomyces boulardii has its highlight to the ability to fight gastrointestinal infections of various kinds. In order to increase the resistance of these microorganisms various microencapsulation techniques have been optimized over the years. This techniques basically consists in coating particles with a material which will provide him protection. The spray-drying technique is one methods that can be employed in microencapsulation. The aim of this study was to develop microcapsules with the probiotic yeast Saccharomyces boulardii using different proteins and their combination with maltodextrin as wall materials. First, they were studied 3 different broths for S. boulardii in order to select the one that provides greater amount of viable cells. Subsequently the cells were microencapsulated in 4 different types of proteins, as well the maltodextrin combinations. This process demonstrated that, among the proteins used, rice protein was the one that provided the highest survival rate of S. boulardii of 98%. The influence of drying temperature, percentage of maltodextrin and feed rate on the survival of S. boulardii were investigated from a factorial 23 experimental design. Tests performed after microencapsulation showed that microcapsules remained stable by At least 15 days after microencapsulation, FTIR analyzes show that there is little interaction between yeast cells and encapsulating agents and thermogravimetric analysis showed that microcapsules are quite resistant to heat. Showing that microencapsulation by spray drying of S. boulardii with different kinds of proteins mixed with maltodextrin is a good method.
publishDate 2017
dc.date.issued.fl_str_mv 2017-03-10
dc.date.accessioned.fl_str_mv 2022-06-07T17:39:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SAVOLDI, Tárcio Enrico. Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina. 2017. 81 f. Dissertação (Mestrado em Engenharia Química) - Universidade Estadual do Oeste do Paraná, Toledo, 2017.
dc.identifier.uri.fl_str_mv https://tede.unioeste.br/handle/tede/6074
identifier_str_mv SAVOLDI, Tárcio Enrico. Microencapsulação de Saccharomyces boulardii por spray-drying com diferentes proteínas combinadas com maltodextrina. 2017. 81 f. Dissertação (Mestrado em Engenharia Química) - Universidade Estadual do Oeste do Paraná, Toledo, 2017.
url https://tede.unioeste.br/handle/tede/6074
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 1582274381427649589
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -7734402124082146922
dc.relation.cnpq.fl_str_mv -1848640261096870878
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Toledo
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Engenharia Química
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Centro de Engenharias e Ciências Exatas
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Toledo
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE
instname:Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron:UNIOESTE
instname_str Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron_str UNIOESTE
institution UNIOESTE
reponame_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
collection Biblioteca Digital de Teses e Dissertações do UNIOESTE
bitstream.url.fl_str_mv http://tede.unioeste.br:8080/tede/bitstream/tede/6074/2/Tarcio_Savoldi_2017.pdf
http://tede.unioeste.br:8080/tede/bitstream/tede/6074/1/license.txt
bitstream.checksum.fl_str_mv 5c29e577754b06f1835c92dca33e0f2c
bd3efa91386c1718a7f26a329fdcb468
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)
repository.mail.fl_str_mv biblioteca.repositorio@unioeste.br
_version_ 1801124702358339584