Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual do Oeste do Parana
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação Stricto Sensu em Bioenergia
|
Departamento: |
Biocombustíveis
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.unioeste.br:8080/tede/handle/tede/1795 |
Resumo: | This study aimed to assess the potential growth of two species of microalgae ( Chlorella vulgaris and Scenedesmus subspicatus ) in two types of effluents ( humans and pigs ) in order to obtain lipids that could be converted into biodiesel . Such compounds must meet the specifications contained in Resolution No. 07 / 2008 of ANP . The effluents were collected in human Station Wastewater Treatment III , located in the city of Foz do Iguaçu / PR and swine effluent in Granja São Jorge ( Colombari ) , located in the municipality of São Miguel do Iguaçu / PR . Both were sent to laboratories for analysis IAP and NUCLEOTEC constatassem that the presence of nitrogenous and phosphatic and oils and greases total , respectively. The effluents were diluted to previously autoclaved and which can serve as culture media for microorganisms . They were then inoculated samples of each type of microalgae in an effluent and evaluated their growth. In four vials was added the compound mineral Agrostemin ® to assess their influence on the results . Prepared growth curves after 60 days of cultivation samples were filtered under vacuum for further extraction of oils using ultrasound. The samples were sent for analysis of lipid content and composition in terms of fatty acids by gas chromatography , which demonstrated the presence of fatty acids that make up biodiesel . The physico-chemical characterization of the effluent showed the presence of small amounts of fats and greases in both the total effluent . The quantitative analysis of lipids showed the highest concentration in the cultivation of C. vulgaris in human effluent , with 50.12 % . With the addition of Agrostemin ®, the yield was better in the cultivation of S. subspicatus in swine wastewater , with 80% increase in the production of lipids , followed by the growth of C. vulgaris in human effluent , with 41 % . The acids were higher concentrations linoleic (C18 : 2) , the cultivation of C. vulgaris in human effluent with 35.17 % , followed by the arachidic ( 20:0 ) with the same microalgae , now in swine wastewater , with 37.29 % , the palmitoleic ( C16 : 1 ) arising from the cultivation of S. subspicatus in human effluent , with 28.85% and pentadecanoic ( C15 : 1 ), the culture of the same organism in swine wastewater , with 55.22 % . It was found that the latter is what gives the best results, but the viability of the remainder is also promising. |
id |
UNIOESTE-1_58eba5a2dd1eb700b57a7d858005fcb0 |
---|---|
oai_identifier_str |
oai:tede.unioeste.br:tede/1795 |
network_acronym_str |
UNIOESTE-1 |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UNIOESTE |
repository_id_str |
|
spelling |
Souza, Samuel Nelson Melegari deCPF:12185394886http://lattes.cnpq.br/0938805013383423Sébastien, Nyamien YahautCPF:48030830378http://lattes.cnpq.br/2977095230414649Bariccatti, Reinaldo AparecidoCPF:08813157886http://lattes.cnpq.br/8065417966435303Silva, Edson Antônio Alves daCPF:64656675920http://lattes.cnpq.br/9304493875700070CPF:84048506749http://lattes.cnpq.br/8045598264951989Novaes Junior, Sidney de Oliveira2017-07-10T17:59:28Z2014-02-202013-08-09NOVAES JUNIOR, Sidney de Oliveira. Potencial use of microalgae Chlorella vulgaris and Scenedesmus subspicatus as raw material for biodiesel production. 2013. 64 f. Dissertação (Mestrado em Biocombustíveis) - Universidade Estadual do Oeste do Parana, Toledo, 2013.http://tede.unioeste.br:8080/tede/handle/tede/1795This study aimed to assess the potential growth of two species of microalgae ( Chlorella vulgaris and Scenedesmus subspicatus ) in two types of effluents ( humans and pigs ) in order to obtain lipids that could be converted into biodiesel . Such compounds must meet the specifications contained in Resolution No. 07 / 2008 of ANP . The effluents were collected in human Station Wastewater Treatment III , located in the city of Foz do Iguaçu / PR and swine effluent in Granja São Jorge ( Colombari ) , located in the municipality of São Miguel do Iguaçu / PR . Both were sent to laboratories for analysis IAP and NUCLEOTEC constatassem that the presence of nitrogenous and phosphatic and oils and greases total , respectively. The effluents were diluted to previously autoclaved and which can serve as culture media for microorganisms . They were then inoculated samples of each type of microalgae in an effluent and evaluated their growth. In four vials was added the compound mineral Agrostemin ® to assess their influence on the results . Prepared growth curves after 60 days of cultivation samples were filtered under vacuum for further extraction of oils using ultrasound. The samples were sent for analysis of lipid content and composition in terms of fatty acids by gas chromatography , which demonstrated the presence of fatty acids that make up biodiesel . The physico-chemical characterization of the effluent showed the presence of small amounts of fats and greases in both the total effluent . The quantitative analysis of lipids showed the highest concentration in the cultivation of C. vulgaris in human effluent , with 50.12 % . With the addition of Agrostemin ®, the yield was better in the cultivation of S. subspicatus in swine wastewater , with 80% increase in the production of lipids , followed by the growth of C. vulgaris in human effluent , with 41 % . The acids were higher concentrations linoleic (C18 : 2) , the cultivation of C. vulgaris in human effluent with 35.17 % , followed by the arachidic ( 20:0 ) with the same microalgae , now in swine wastewater , with 37.29 % , the palmitoleic ( C16 : 1 ) arising from the cultivation of S. subspicatus in human effluent , with 28.85% and pentadecanoic ( C15 : 1 ), the culture of the same organism in swine wastewater , with 55.22 % . It was found that the latter is what gives the best results, but the viability of the remainder is also promising.Este trabalho teve como objetivo avaliar o potencial de crescimento de duas espécies de microalgas (Chlorella vulgaris e Scenedesmus subspicatus) em dois tipos de efluentes (humanos e suínos) com a finalidade de se obter lipídios que pudessem ser convertidos em biodiesel. Tais compostos precisam atender às especificações contidas na Resolução nº 07/2008 da ANP. Os efluentes humanos foram coletados na Estação de Tratamento de Efluentes III, localizada na cidade de Foz do Iguaçu/PR e os efluentes suínos na Granja São Jorge (Colombari), localizada no município de São Miguel do Iguaçu/PR. Ambos foram enviados aos laboratórios do IAP e NUCLEOTEC para análises que constatassem a presença de componentes nitrogenados e fosfatados e de óleos e graxas totais, respectivamente. Os efluentes foram previamente autoclavados e diluídos para que pudessem servir de meios de cultura para os microrganismos. Foram, então, inoculadas amostras de cada microalga em um tipo de efluente e avaliado o seu crescimento. Em quatro frascos foi acrescentado o composto mineral Agrostemin® para avaliar sua influência nos resultados. Elaboradas as curvas de crescimento, após 60 dias de cultivo as amostras foram filtradas a vácuo para posterior extração dos óleos utilizando ultrassom. As amostras foram enviadas para análise do teor de lipídios e sua composição em termos de ácidos graxos por cromatografia gasosa, onde se constatou a presença de ácidos graxos que compõem o biodiesel. A caracterização físico-química dos efluentes constatou a presença de pequena quantidade de gorduras e graxas totais em ambos os efluentes. A análise quantitativa dos lipídios apontou a maior concentração no cultivo de C. vulgaris em efluentes humanos, com 50,12%. Com a adição do Agrostemin®, o melhor rendimento foi no cultivo de S. subspicatus em efluentes suínos, com aumento de 80% na produção de lipídios, seguido do cultivo de C. vulgaris em efluentes humanos, com 41%. Os ácidos com maiores concentrações foram o linoleico (C18:2), do cultivo de C. vulgaris em efluente humano com 35,17%, seguido do araquídico (20:0) com a mesma microalga, agora em efluente suíno, com 37,29%, o palmitoleico (C16:1) oriundo do cultivo de S. subspicatus em efluente humano, com 28,85% e o pentadecanoico (C15:1), da cultura do mesmo microrganismo em efluente suíno, com 55,22%. Constatou-se que este último é o que apresenta os melhores resultados, mas a viabilidade do restante é igualmente promissora.Made available in DSpace on 2017-07-10T17:59:28Z (GMT). No. of bitstreams: 1 Sidney de O Novaes Junior.pdf: 6661390 bytes, checksum: db911a668b5af886529a0d861278a4c1 (MD5) Previous issue date: 2013-08-09application/pdfporUniversidade Estadual do Oeste do ParanaPrograma de Pós-Graduação Stricto Sensu em BioenergiaUNIOESTEBRBiocombustíveisBiodieselMicroalgasEfluentesLipídiosBioenergiaBiodiesel de microalgasBiocombustíveisBiotecnologiaEnergia de biomassaMicroalga - Cultura e meios de culturaClorella vulgarisScenedesmus subpicatusBiodieselMicroalgaeEffluent lipidsCNPQ::ENGENHARIAS::ENGENHARIA QUIMICAPotencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodieselPotencial use of microalgae Chlorella vulgaris and Scenedesmus subspicatus as raw material for biodiesel productioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALSidney de O Novaes Junior.pdfapplication/pdf6661390http://tede.unioeste.br:8080/tede/bitstream/tede/1795/1/Sidney+de+O+Novaes+Junior.pdfdb911a668b5af886529a0d861278a4c1MD51tede/17952017-07-10 14:59:28.308oai:tede.unioeste.br:tede/1795Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2017-07-10T17:59:28Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false |
dc.title.por.fl_str_mv |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel |
dc.title.alternative.eng.fl_str_mv |
Potencial use of microalgae Chlorella vulgaris and Scenedesmus subspicatus as raw material for biodiesel production |
title |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel |
spellingShingle |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel Novaes Junior, Sidney de Oliveira Biodiesel Microalgas Efluentes Lipídios Bioenergia Biodiesel de microalgas Biocombustíveis Biotecnologia Energia de biomassa Microalga - Cultura e meios de cultura Clorella vulgaris Scenedesmus subpicatus Biodiesel Microalgae Effluent lipids CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel |
title_full |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel |
title_fullStr |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel |
title_full_unstemmed |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel |
title_sort |
Potencial de aproveitamento de microalgas Chlorella vulgaris e Scenedesmus subspicatus como matéria-prima para obtenção de biodiesel |
author |
Novaes Junior, Sidney de Oliveira |
author_facet |
Novaes Junior, Sidney de Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Souza, Samuel Nelson Melegari de |
dc.contributor.advisor1ID.fl_str_mv |
CPF:12185394886 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0938805013383423 |
dc.contributor.advisor-co1.fl_str_mv |
Sébastien, Nyamien Yahaut |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:48030830378 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2977095230414649 |
dc.contributor.referee1.fl_str_mv |
Bariccatti, Reinaldo Aparecido |
dc.contributor.referee1ID.fl_str_mv |
CPF:08813157886 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8065417966435303 |
dc.contributor.referee2.fl_str_mv |
Silva, Edson Antônio Alves da |
dc.contributor.referee2ID.fl_str_mv |
CPF:64656675920 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9304493875700070 |
dc.contributor.authorID.fl_str_mv |
CPF:84048506749 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8045598264951989 |
dc.contributor.author.fl_str_mv |
Novaes Junior, Sidney de Oliveira |
contributor_str_mv |
Souza, Samuel Nelson Melegari de Sébastien, Nyamien Yahaut Bariccatti, Reinaldo Aparecido Silva, Edson Antônio Alves da |
dc.subject.por.fl_str_mv |
Biodiesel Microalgas Efluentes Lipídios Bioenergia Biodiesel de microalgas Biocombustíveis Biotecnologia Energia de biomassa Microalga - Cultura e meios de cultura Clorella vulgaris Scenedesmus subpicatus |
topic |
Biodiesel Microalgas Efluentes Lipídios Bioenergia Biodiesel de microalgas Biocombustíveis Biotecnologia Energia de biomassa Microalga - Cultura e meios de cultura Clorella vulgaris Scenedesmus subpicatus Biodiesel Microalgae Effluent lipids CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Biodiesel Microalgae Effluent lipids |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
description |
This study aimed to assess the potential growth of two species of microalgae ( Chlorella vulgaris and Scenedesmus subspicatus ) in two types of effluents ( humans and pigs ) in order to obtain lipids that could be converted into biodiesel . Such compounds must meet the specifications contained in Resolution No. 07 / 2008 of ANP . The effluents were collected in human Station Wastewater Treatment III , located in the city of Foz do Iguaçu / PR and swine effluent in Granja São Jorge ( Colombari ) , located in the municipality of São Miguel do Iguaçu / PR . Both were sent to laboratories for analysis IAP and NUCLEOTEC constatassem that the presence of nitrogenous and phosphatic and oils and greases total , respectively. The effluents were diluted to previously autoclaved and which can serve as culture media for microorganisms . They were then inoculated samples of each type of microalgae in an effluent and evaluated their growth. In four vials was added the compound mineral Agrostemin ® to assess their influence on the results . Prepared growth curves after 60 days of cultivation samples were filtered under vacuum for further extraction of oils using ultrasound. The samples were sent for analysis of lipid content and composition in terms of fatty acids by gas chromatography , which demonstrated the presence of fatty acids that make up biodiesel . The physico-chemical characterization of the effluent showed the presence of small amounts of fats and greases in both the total effluent . The quantitative analysis of lipids showed the highest concentration in the cultivation of C. vulgaris in human effluent , with 50.12 % . With the addition of Agrostemin ®, the yield was better in the cultivation of S. subspicatus in swine wastewater , with 80% increase in the production of lipids , followed by the growth of C. vulgaris in human effluent , with 41 % . The acids were higher concentrations linoleic (C18 : 2) , the cultivation of C. vulgaris in human effluent with 35.17 % , followed by the arachidic ( 20:0 ) with the same microalgae , now in swine wastewater , with 37.29 % , the palmitoleic ( C16 : 1 ) arising from the cultivation of S. subspicatus in human effluent , with 28.85% and pentadecanoic ( C15 : 1 ), the culture of the same organism in swine wastewater , with 55.22 % . It was found that the latter is what gives the best results, but the viability of the remainder is also promising. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-08-09 |
dc.date.available.fl_str_mv |
2014-02-20 |
dc.date.accessioned.fl_str_mv |
2017-07-10T17:59:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOVAES JUNIOR, Sidney de Oliveira. Potencial use of microalgae Chlorella vulgaris and Scenedesmus subspicatus as raw material for biodiesel production. 2013. 64 f. Dissertação (Mestrado em Biocombustíveis) - Universidade Estadual do Oeste do Parana, Toledo, 2013. |
dc.identifier.uri.fl_str_mv |
http://tede.unioeste.br:8080/tede/handle/tede/1795 |
identifier_str_mv |
NOVAES JUNIOR, Sidney de Oliveira. Potencial use of microalgae Chlorella vulgaris and Scenedesmus subspicatus as raw material for biodiesel production. 2013. 64 f. Dissertação (Mestrado em Biocombustíveis) - Universidade Estadual do Oeste do Parana, Toledo, 2013. |
url |
http://tede.unioeste.br:8080/tede/handle/tede/1795 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Parana |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação Stricto Sensu em Bioenergia |
dc.publisher.initials.fl_str_mv |
UNIOESTE |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biocombustíveis |
publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Parana |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE instname:Universidade Estadual do Oeste do Paraná (UNIOESTE) instacron:UNIOESTE |
instname_str |
Universidade Estadual do Oeste do Paraná (UNIOESTE) |
instacron_str |
UNIOESTE |
institution |
UNIOESTE |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UNIOESTE |
collection |
Biblioteca Digital de Teses e Dissertações do UNIOESTE |
bitstream.url.fl_str_mv |
http://tede.unioeste.br:8080/tede/bitstream/tede/1795/1/Sidney+de+O+Novaes+Junior.pdf |
bitstream.checksum.fl_str_mv |
db911a668b5af886529a0d861278a4c1 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE) |
repository.mail.fl_str_mv |
biblioteca.repositorio@unioeste.br |
_version_ |
1794618455487414272 |