Avaliação in vitro e in vivo do impacto e eficiência da remoção do pesticida pirofosfato de tetraetilo (TEPP) em água com a utilização de produto de origem natural com propriedades adsorventes

• Ano de defesa: 2024
• Autor(a) principal: Malheiros, Fernanda Blini Marengo
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Adsorvente; Acetilcolinesteras; Organofosforados; Pesticidas; Adsorbent; Acetylcholinesterase; Organophosphates; Pesticides
• Link de acesso: https://hdl.handle.net/11449/255719

Brazil is in the ranking of the world's largest consumers of pesticides. This contributes to the intensification of exposure of the population and the environment to these dangerous substances. The issue of this work is relevant to the assessment and minimization of the environmental impacts of agro-industrial activities caused by the use of the contaminant tetraethyl pyrophosphate (TEPP), used in coffee, beans and soybean crops, from the use of zebrafish embryos (Danio rerio). TEPP is an organophosphate pesticide that irreversibly inhibits acetylcholinesterase (AChE). Thus, the present study, with an innovative proposal, evaluated the impact of the pesticide TEPP on the activity of acetylcholinesterase in PC12 neuronal cells (in vitro) and compared, in vivo, the efficiency of removing the pesticide from water with the use of cork granules as a natural adsorbent in a species of zebrafish, a fish very sensitive to environmental changes and adversities, in order to analyze the toxicological effects of TEPP as a contaminant on the development and behavior of embryos and juveniles. TEPP inhibited AChE activity in a dose-dependent manner. For the first time, it was shown in vitro that different concentrations of TEPP diluted in water after adsorption experiments using cork granules decreased the inhibitory effects of TEPP on AChE activity in commercial enzyme and PC12 neuronal cell culture medium. Furthermore, the toxicological effects induced by TEPP on the development and behavior of zebrafish and on the possible decontamination of water using cork granules with adsorbent properties were evaluated. Exposure to TEPP decreased the viability rate of embryos. In conclusion, our research suggests that zebrafish at different developmental stages were able to detect TEPP-induced toxicological effects at nanomolar concentrations, which is more sensible than experiments performed in vitro.