Proliferação celular em gestações naturais e de conceptos bovinos transgênicos e clonados, que expressam a proteína fluorescente verde (GFP)

• Ano de defesa: 2015
• Autor(a) principal: Oliveira, Fernanda Paes de [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Genetica animal; Clonagem; Animais transgênicos; Placenta; Animal genetics
• Link de acesso: http://hdl.handle.net/11449/126388; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/13-08-2015/000843813.pdf

For the placental growth and fetal nutrition, high rates of growth and differentiation are necessary for a proper balance between proliferation and cell death of placental cells. These cells have specific properties in relation to its metabolic, endocrine and angiogenic function and they are fundamental to the development of the fetus throughout pregnancy. In this study, the occurrence of cell proliferation was assessed in placentomes by cloned transgenic bovine conceptuses (n=5) and by artificial insemination (AI), in the 60 and 90 days of gestation, and they development were interrupted and recovering the pregnant uterus. The samples were cut and fixed in paraformaldehyde 4% aqueous solution in sodium phosphate buffer (PBS) 0.1M at pH 7.4, for verification of the morphology and performing immunohistochemistry. The results were compared with bovine cloned and AI. The reults obtained were compared between transgenic cloned bovine conceptuses and AI. In all groups and gestational periods, the fetal epithelium presents positive labeling to cell proliferation. In 60 days gestation the positive labeling of the manipulated gestations was less evident in relation to the natural gestations. On the other hand, in 90 days gestation the immunoreactivity of the placentomes of the cloned conceptuses was more intense, not just in the epithelium, but also in the fetal connective tissue, fact not observed in the natural gestation, were the positive reaction was less evident in the fetal connective tissue. In this study was noted a possible imbalance in the patterns of the cell proliferation in the transgenic cloned bovine conceptuses, because in the early gestation (60 days) they presented a lesser proliferative activity, and in the 90 days a increase, may be this fact one of the factors leading to placental abnormalities. Therefore, the results of verification of cell proliferation and understanding are important for the understanding of possible fails...