Atividade antibacteriana de cimentos de ionômero de vidro modificados por nanopartículas de óxido de zinco

• Ano de defesa: 2014
• Autor(a) principal: Bell, Mariana Florian [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Agentes antibacterianos; Cimentos de ionômeros de vidro; Nanopartículas; Nanoparticles
• Link de acesso: http://hdl.handle.net/11449/113937

This study evaluated the antibacterial activity of different concentrations of zinc oxide nanoparticles incorporated into a self-cured glass ionomer cement (GIC) and a light-cured resin-reinforced glass ionomer cement on Streptococcus mutans biofilm at different maturation times. Nanoparticles concentrations were 0%, 1% e 2%, the GICs were Fuji II (GC America) and Fuji II LC (GC America) and the maturation times were 24 hours and 7 days. Were prepared circular (4mm x 2mm) specimens (N= 108). The antibacterial activity was evaluated by the number of Colony Forming Unit per militers (CFU/ml). Morphological analysis of biofilm was taken by scanning electron microscopy (SEM). The data obtained for each material attended the assumptions of normality and homoscedasticity and a two-way ANOVA ( =5%). No significant difference was observed according to the time of biofilm maturation (p=0.744), concentration of nanoparticles (p=0.966) and their interaction (p=0.800). Resin-reinforced GIC showed significantly higher CFU/ml mean for 7-day biofilms compared to 24h-biofilms (p=0.034). The concentration of nanoparticles (p=0.570) and the interaction (p=0.756) between the two factors was not significant. SEM analysis showed no change in cell morphology of S. mutans according to the type of GIC, maturation time and nanoparticles concentration. We conclude that the inclusion of zinc oxide nanoparticles at concentrations of 1% and 2% into the glass ionomer cement evaluated did not promote antimicrobial activity against Streptococcus mutans.