Caracterização do groundnut ringspot virus (grsv) e seu vetor (Frankliniella schultzei) em melancia

• Ano de defesa: 2015
• Autor(a) principal: Leão, Evelynne Urzêdo [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Melancia - Doenças e pragas; Cucurbitacea - Resistencia a doenças e pragas - Aspectos geneticos; Reação em cadeia de polimerase; Watermelons Diseases and pests
• Link de acesso: http://hdl.handle.net/11449/126555; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/10-08-2015/000844621.pdf

Watermelon (Citrullus lanatus) is considered one of the most important cucurbits in the world and Brazil is the fourth largest producer, with a total cultivated area of 90.000 ha and average production of about two million tons. In Brazil, viruses described in watermelon belong to the genus Potyvirus and Cucumovirus and Zucchini lethal chlorosis virus is the only species of Tospovirus genre described on this culture. Considering the high incidence of watermelon plants showing symptoms caused by virus during the 2012/2013 seasons and the presence of thrips in the field, the virus and the vector associated with the disease was identified and characterized. In the first chapter entitled Citrullus lanatus is a new natural host of Groundnut ringspot virus in Brazil, the species of tospovirus Groundnut ringspot virus(GRSV) was reported causing mosaic, deformation and necrotic lesions on leaves and fruits of watermelon. The GRSV was collected during the years 2012 and 2013 in producing areas of Presidente Prudente and Marília. GRSV from watermelon, differently from GRSV strains found on solanaceas, didn't infected tomato plants cv. Santa Clara and Mariana. Thrips specimens collected in the field were able to transmit GRSV to watermelon, peppers and Nicotiana species. This was the first report of natural infection of watermelon by GRSV. In the second chapter entitled A Taqman real-time RT - PCR assay for detection of Groundnut ringspot virus a quantitative PCR test was developed to efficiently detect GRSV from watermelon and from individual thrips. The test might be useful to detect GRSV and for further studies with this virus. The third chapter Efficient detection of Frankliniella schultzei (Thripidae: Thysanoptera) by real-time PCR reports the efficient detection of Frankliniella schultzei by the analysis of the mitochondrial gene cytochrome oxidase I (mtCOI). Thrips samples were ...