Análise de carga viral e estado físico do Papilomavírus Humano (HPV) e perfil de citocinas em mulheres com lesão intraepitelial cervical e carconoma cervical invasor

• Ano de defesa: 2015
• Autor(a) principal: Marcolino, Larissa Doddi [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Virus do papiloma; Doenças sexualmente transmissíveis; Colo uterino - Câncer; Citocinas; Papillomaviridae
• Link de acesso: http://hdl.handle.net/11449/143005

Introduction: Infection with high-risk genotypes of the human papillomavirus (HPV) is associated with the development of preinvasive cervical lesions and invasive cervical carcinoma. The viral load and physical state of HPV genotypes are considered important biomarkers for lesion progression. The immune response has an important role in the natural history of HPV infection in the uterine cervix, as, for example, the viral persistence and the development of cervical cancer. Cytokines are the main mediators of this immune response and they regulate the HPV transcription depending on the response pattern to which they are related. The Th1 pattern contributes to the development of cellular immunity against HPV infection and its clearance. On the other hand, Th2 pattern is associated with the persistence of viral infection and the progression of cervical lesions. Most of these infections probably involves a balance between Th1 and Th2 immune response profiles.Objective: To evaluate the concentration of IL-1 β, IL-4, IL-6, IL-10,TNF-and INF-in secretionand serum and also to assess the immunostaining intensity of these cytokines in cervical biopsies.Moreover, the present study aimed to determine the viral load and physical state of HPV16 in women with low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), invasive cervical carcinoma (ICC) and also in the control group.Patients and Methods: This study included 109 women with histopathologic diagnosis of LSIL (n=16), HSIL (n=40), ICC (n=13) and 40 women with no pathological changes on cervix biopsy (control group) attended in Preventive Gynaecological Ambulatory at the Amaral Carvalho Hospital, Jaú, São Paulo, Brazil. Cytokine concentrationwas determined in serum and cervical secretion by enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry was used to evaluate the immunostaining intensity of cytokines in biopsy ...