Uso do ômega 3 oral em diferentes proporções de EPA e DHA associado com antioxidantes como adjuvante no tratamento de ceratoconjuntivite seca em cães

• Ano de defesa: 2018
• Autor(a) principal: Silva, Danielle Alves
• Orientador(a): Andrade, Silvia Maria Caldeira Franco
• Banca de defesa: Pacagnelli, Francis Lopes , Nogueira, Rosa Maria Barilli , Brandão, Cláudia Valéria Seullner , Ranzani, José Joaquim Titton
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Oeste Paulista
• Programa de Pós-Graduação: Doutorado em Fisiopatologia e Saúde Animal
• Departamento: Doutorado em Fisiopatologia e Saúde Animal
• País: Brasil
• Palavras-chave em Português: Ceratoconjuntivite seca, ômega 3, EPA, DHA, antioxidantes, cães, análise fractal.
• Palavras-chave em Inglês: Dry eye, tacrolimus, omega, EPA, DHA, dogs, fractal analysis.
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://bdtd.unoeste.br:8080/jspui/handle/jspui/1090

The objective of this study was to compare the efficacy of two omega 3 oral formulations with different ratios of EPA, DHA and antioxidants, as an adjuvant in the treatment of dogs affected by KCS and to evaluate by fractal analysis the palpebral conjunctiva of the treated dogs. Forty-five dogs with KCS were evaluated monthly for 6 months by the Schirmer Tear Test (TLS), Fluorescein Test (TF), Tear Film Break-up Time (TBUT), Lissamine Green Test (LGT), cytology of the conjunctiva at the beginning, middle and end of the study, biopsy and fractal analysis of the conjunctiva at the beginning and end of the study. The dogs were randomly assigned into 3 groups (n = 15): T group (tacrolimus 0.03% topical), TO group (tacrolimus + omegas EPA/DHA) and TOA group (tacrolimus + omegas EPA/DHA + Antioxidants). The result demonstrated that there was a significant improvement in clinical signs in both groups. In TBUT, all groups presented increase during the treatment, and the TO group presented the best result at all times when compared to the other groups. At the end of the experiment, the groups T, TO and TOA presented cytological analysis, reduction of lymphocytes, neutrophils, metaplastic and squamous cells, and histopathological analysis, reduction of lymphocytes and neutrophils and increase of goblet cells highlighting the best performance at TO. In the fractal analysis, at the end of the experiment, the TO group presented the best result and the values close to the values found in the fractals of healthy eyes. The difference between the TO group and the other groups is in the higher EPA concentration, being a natural anti-inflammatory, which may be one of the causes of its better performance. We concluded that oral omega 3, which contains a higher proportion of EPA than DHA, has shown greater benefit in terms of the improvement of clinical signs and the inflammatory process in the treatment of KCS in dogs.