Utilização de plasma rico em plaquetas e rosuvastatina no processo cicatricial de feridas de coelhos: estudo longitudinal

• Ano de defesa: 2018
• Autor(a) principal: Tetila, Amanda Feba
• Orientador(a): Laposy, Cecília Braga
• Banca de defesa: Camargo Filho, José Carlos Silva , Pacagnelli, Francis Lopes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Oeste Paulista
• Programa de Pós-Graduação: Mestrado em Ciência Animal
• Departamento: Mestrado em Ciência Animal
• País: Brasil
• Palavras-chave em Português: Cicatrização, Plasma Rico em Plaquetas, Rosuvastatina Cálcica
• Palavras-chave em Inglês: Cicatrization, Platelet- rich Plasma, Rosuvastatin Calcica
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://bdtd.unoeste.br:8080/jspui/handle/jspui/1126

Tissue engineering, as well as regenerative medicine, are fields that have been growing in recent years, aiming to repair, regenerate or replace damaged tissues and organs. PRP has been extensively studied in the areas of plastic surgery and dermatology with a focus on scar remodeling. Numerous studies with are still being done in search of new findings related to this blood element. Evidence is proving to favor the use of statins in skin lesions. In addition to lowering cholesterol, statins improve endothelial function, increase the stability of atherosclerotic plaques, and inhibit the inflammatory process. In this sense, the present study analyzed the collagen fibers of rabbit wounds after PRP treatment associated or not to RSV. The hypothesis is that serial use of RSV associated with PRP may improve the amount of neoformed collagen fibers after experimentally induced injury. 8 male New Zealand rabbits treated with autologous PRP, rosuvastatin (RSV) and association (PRP + RSV) were used to prepare the four wounds on the back of each rabbit. The treated wound was treated with 0.9% sodium chloride solution and the others treated with PRP isolated and / or associated with RSV. The dressings were changed every 4 days with application of PRP and rosuvastatin until 16 days of experiment. Biopsies were made at 0,7,10,14 and 17 days for histopathological evaluation of the scar tissue. Regarding the histopathological evaluation, at the end of 17 days of treatment, there was re-epithelialization in 100% of the wounds that received PRP only, opposing with 50% only with rosuvastatin and 75% when there was an association of the biomaterial with the statin. The combination of rosuvastatin and PRP inhibited blood loss. The use of PRP alone provided 62.5% of neovascularization, against 50% in wounds that had association of treatments. With regard to the percentage of collagen fibers at 170 days, all wounds had a higher percentage of fibers when compared to the control (78.27 ± 4.69). Although statistically equal, it was observed that the wound that received only RSV had the lowest amount of collagen fibers (85.98 ± 3.51). Wounds that received PRP or PRP and RSV presented 90.07 ± 6.20 and 90.76 ± 3.51, respectively. It is concluded that the PRP has greater activity in the first seven days of the lesion and that the treatment with RSV, alone or associated with PRP did not potentiate the other phases of healing, nor did it increase the percentage of collagen fibers.