Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos
| Ano de defesa: | 2023 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | , , , |
| Tipo de documento: | Tese |
| Tipo de acesso: | Acesso aberto |
| Idioma: | por |
| Instituição de defesa: |
Universidade do Oeste Paulista
|
| Programa de Pós-Graduação: |
Doutorado em Fisiopatologia e Saúde Animal
|
| Departamento: |
Doutorado em Fisiopatologia e Saúde Animal
|
| País: |
Brasil
|
| Palavras-chave em Português: | |
| Palavras-chave em Inglês: | |
| Área do conhecimento CNPq: | |
| Link de acesso: | http://bdtd.unoeste.br:8080/jspui/handle/jspui/1617 |
Resumo: | Annatto has two main carotenoids in its composition, bixin, with red color, and norbixin, with yellow color. Carotenoids have antioxidant substances in their composition, which minimize the effects of free radicals and inhibit the oxidative degradation of lipids (lipid peroxidation), which can cause harmful damage to health, in addition to having anti-inflammatory and antioxidant effects. However, it is observed that these properties have not yet been explored, with only the use of the product being limited to dyes. With that in mind, the objective of the present study is to evaluate the use of annatto concentrate with a high content of bixin as an antioxidant source in the diet of ewes at different reproductive stages, evaluating its effects on oxidative stress and its use as an antioxidant in the diet of feedlot lambs. evaluating its effects on meat lipid oxidation and shelf life. For this, 30 crossbred Dorper ewes were divided into 3 groups: 0%, 0.5% and 1% of annatto concentrate in the diet, with blood collected from these animals in the prepartum, postpartum and weaning of the lambs. , processed to obtain washed red blood cells and blood plasma and performed analyzes of concentration of FRAP, TBAR's, hydrogen peroxide and reduced thiols and the activity of the enzymes glutathione peroxidase, superoxide dismutase and catalase. In the confinement of the lambs, 32 male lambs were used, divided into 4 treatments: 0%, 0.25%, 0.50% and 0.75% of annatto concentrate added to the feed provided. After reaching 42 kg of live weight, the animals were slaughtered and the portion of the Longissimus lumborum muscle was collected for analysis, where the meat was divided into 3 cm pieces and placed on trays, covered by plastic film, and lipid oxidation analysis was performed (TBAR's) and FRAP on days 0, 3, 7 and 14 of shelf life, concomitantly a subjective evaluation of consumers was carried out, where every 4 days they evaluated the acceptance of the samples by the color of the meat. With the studies it was concluded that the use of annatto concentrate in the diet of sheep can reduce oxidative stress in ewes at different reproductive stages and manages to control the lipid oxidation of the meat until the 7th day of shelf life, functioning as an antioxidant agent. |
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Zundt, MariliceCastilho, Anthony César de SouzaCastilho, CaliêDamasceno, DéboraMarques, RodrigoSouza, Leticia Jalloul Guimarães de2024-10-10T17:10:28Z2023-09-25Souza, Leticia Jalloul Guimarães de. Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos. 2023. 85f. Tese (Doutorado em Fisiopatologia e Saúde Animal) - Universidade do Oeste Paulista, Presidente Prudente.http://bdtd.unoeste.br:8080/jspui/handle/jspui/1617Annatto has two main carotenoids in its composition, bixin, with red color, and norbixin, with yellow color. Carotenoids have antioxidant substances in their composition, which minimize the effects of free radicals and inhibit the oxidative degradation of lipids (lipid peroxidation), which can cause harmful damage to health, in addition to having anti-inflammatory and antioxidant effects. However, it is observed that these properties have not yet been explored, with only the use of the product being limited to dyes. With that in mind, the objective of the present study is to evaluate the use of annatto concentrate with a high content of bixin as an antioxidant source in the diet of ewes at different reproductive stages, evaluating its effects on oxidative stress and its use as an antioxidant in the diet of feedlot lambs. evaluating its effects on meat lipid oxidation and shelf life. For this, 30 crossbred Dorper ewes were divided into 3 groups: 0%, 0.5% and 1% of annatto concentrate in the diet, with blood collected from these animals in the prepartum, postpartum and weaning of the lambs. , processed to obtain washed red blood cells and blood plasma and performed analyzes of concentration of FRAP, TBAR's, hydrogen peroxide and reduced thiols and the activity of the enzymes glutathione peroxidase, superoxide dismutase and catalase. In the confinement of the lambs, 32 male lambs were used, divided into 4 treatments: 0%, 0.25%, 0.50% and 0.75% of annatto concentrate added to the feed provided. After reaching 42 kg of live weight, the animals were slaughtered and the portion of the Longissimus lumborum muscle was collected for analysis, where the meat was divided into 3 cm pieces and placed on trays, covered by plastic film, and lipid oxidation analysis was performed (TBAR's) and FRAP on days 0, 3, 7 and 14 of shelf life, concomitantly a subjective evaluation of consumers was carried out, where every 4 days they evaluated the acceptance of the samples by the color of the meat. With the studies it was concluded that the use of annatto concentrate in the diet of sheep can reduce oxidative stress in ewes at different reproductive stages and manages to control the lipid oxidation of the meat until the 7th day of shelf life, functioning as an antioxidant agent.O urucum possui dois carotenoides principais na sua composição, a bixina, com coloração vermelha, e a norbixina, com coloração amarela. Os carotenoides apresentam em sua composição substâncias antioxidantes, que minimizam os efeitos de radicais livres e inibem a degradação oxidativa dos lipídeos (peroxidação lipídica), que podem causar danos nocivos à saúde, além de possuírem efeitos anti-inflamatórios e antioxidantes. No entanto, observa-se que estas propriedades ainda não foram exploradas, ficando apenas o uso do produto limitado aos corantes. Pensando nisso o objetivo do presente estudo é avaliar o uso do concentrado de urucum com alto teor de bixina como fonte antioxidante na dieta de ovelhas em diferentes fases reprodutivas, avaliando seus efeitos sobre o estresse oxidativo e seu uso como antioxidante na dieta de cordeiros em confinamento avaliando seus efeitos na oxidação lipídica da carne e tempo de prateleira. Para isso utilizou-se 30 ovelhas mestiças Dorper divididas em 3 grupos sendo eles: 0%, 0,5% e 1% de concentrado de urucum na dieta, sendo colhido sangue desses animais no pré-parto, pós-parto e desmame dos cordeiros, feita seu processamento para obtenção de hemácias lavadas e plasma sanguíneo e realização das análises de concentração de FRAP, TBAR’s, peróxido de hidrogênio e tióis reduzidos e a atividade das enzimas glutationa peroxidase, superóxido dismutase e catalase. No confinamento dos cordeiros, foram utilizados 32 cordeiros machos, divididos em 4 tratamentos sendo eles: 0%, 0,25%, 0,50% e 0,75% de adição de concentrado de urucum na ração fornecida. Após alcançarem 42kg de peso vivo, os animais foram abatidos e a porção do músculo Longissimus lumborum foi coletada para realização da análise, sendo dividido em pedaços de 3 cm e disposta em bandejas, coberta por plástico filme sendo feita análise de oxidação lipídica (TBAR’s) e FRAP nos dias 0, 3, 7 e 14 de tempo de prateleira, concomitante foi realizada avaliação subjetiva de consumidores, que a cada 4 dias avaliavam a aceitação das amostras pela cor da carne. Com os estudos concluiu-se que o uso de concentrado de urucum na dieta de ovinos é capaz de reduzir o estresse oxidativo em ovelhas em diferentes fases reprodutivas e consegue controlar a oxidação lipídica da carne até o dia 7 de tempo de prateleira, funcionando como agente antioxidante.Submitted by Michele Mologni (mologni@unoeste.br) on 2024-10-10T17:10:28Z No. of bitstreams: 1 Leticia Jalloul Guimarães de Souza.pdf: 203492 bytes, checksum: 740c0f6a576ef73304eae8adf0e7f37c (MD5)Made available in DSpace on 2024-10-10T17:10:28Z (GMT). No. of bitstreams: 1 Leticia Jalloul Guimarães de Souza.pdf: 203492 bytes, checksum: 740c0f6a576ef73304eae8adf0e7f37c (MD5) Previous issue date: 2023-09-25application/pdfhttp://bdtd.unoeste.br:8080/jspui/retrieve/5682/Leticia%20Jalloul%20Guimar%c3%a3es%20de%20Souza.pdf.jpgporUniversidade do Oeste PaulistaDoutorado em Fisiopatologia e Saúde AnimalUNOESTEBrasilDoutorado em Fisiopatologia e Saúde Animalcarne; hemácias; oxidação; sangue; TBAR’s.blood; meat; oxidation; red cells; TBAR's.CIENCIAS AGRARIAS::MEDICINA VETERINARIAPotencial antioxidante do urucum (bixa orellana) na dieta de ovinosAntioxidant potential of annato (Bixa orellana) in the diet of sheepinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis56116417490182658795005006005611641749018265879453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNOESTEinstname:Universidade do Oeste Paulista (UNOESTE)instacron:UNOESTETHUMBNAILLeticia Jalloul Guimarães de Souza.pdf.jpgLeticia Jalloul Guimarães de Souza.pdf.jpgimage/jpeg3331http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1617/3/Leticia+Jalloul+Guimar%C3%A3es+de+Souza.pdf.jpg189872e56c278348ad6aa802242c5db1MD53ORIGINALLeticia Jalloul Guimarães de Souza.pdfLeticia Jalloul Guimarães de Souza.pdfapplication/pdf203492http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1617/2/Leticia+Jalloul+Guimar%C3%A3es+de+Souza.pdf740c0f6a576ef73304eae8adf0e7f37cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82067http://bdtd.unoeste.br:8080/tede/bitstream/jspui/1617/1/license.txt47745281809acb27fb322a97f2d9cb88MD51jspui/16172024-10-11 01:07:38.993oai:bdtd.unoeste.br: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 Digital de Teses e Dissertaçõeshttp://bdtd.unoeste.br:8080/jspui/PUBhttp://bdtd.unoeste.br:8080/oai/requestbdtd@unoeste.bropendoar:2024-10-11T04:07:38Biblioteca Digital de Teses e Dissertações da UNOESTE - Universidade do Oeste Paulista (UNOESTE)false |
| dc.title.por.fl_str_mv |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos |
| dc.title.alternative.eng.fl_str_mv |
Antioxidant potential of annato (Bixa orellana) in the diet of sheep |
| title |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos |
| spellingShingle |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos Souza, Leticia Jalloul Guimarães de carne; hemácias; oxidação; sangue; TBAR’s. blood; meat; oxidation; red cells; TBAR's. CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| title_short |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos |
| title_full |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos |
| title_fullStr |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos |
| title_full_unstemmed |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos |
| title_sort |
Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos |
| author |
Souza, Leticia Jalloul Guimarães de |
| author_facet |
Souza, Leticia Jalloul Guimarães de |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Zundt, Marilice |
| dc.contributor.referee1.fl_str_mv |
Castilho, Anthony César de Souza |
| dc.contributor.referee2.fl_str_mv |
Castilho, Caliê |
| dc.contributor.referee3.fl_str_mv |
Damasceno, Débora |
| dc.contributor.referee4.fl_str_mv |
Marques, Rodrigo |
| dc.contributor.author.fl_str_mv |
Souza, Leticia Jalloul Guimarães de |
| contributor_str_mv |
Zundt, Marilice Castilho, Anthony César de Souza Castilho, Caliê Damasceno, Débora Marques, Rodrigo |
| dc.subject.por.fl_str_mv |
carne; hemácias; oxidação; sangue; TBAR’s. |
| topic |
carne; hemácias; oxidação; sangue; TBAR’s. blood; meat; oxidation; red cells; TBAR's. CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| dc.subject.eng.fl_str_mv |
blood; meat; oxidation; red cells; TBAR's. |
| dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| description |
Annatto has two main carotenoids in its composition, bixin, with red color, and norbixin, with yellow color. Carotenoids have antioxidant substances in their composition, which minimize the effects of free radicals and inhibit the oxidative degradation of lipids (lipid peroxidation), which can cause harmful damage to health, in addition to having anti-inflammatory and antioxidant effects. However, it is observed that these properties have not yet been explored, with only the use of the product being limited to dyes. With that in mind, the objective of the present study is to evaluate the use of annatto concentrate with a high content of bixin as an antioxidant source in the diet of ewes at different reproductive stages, evaluating its effects on oxidative stress and its use as an antioxidant in the diet of feedlot lambs. evaluating its effects on meat lipid oxidation and shelf life. For this, 30 crossbred Dorper ewes were divided into 3 groups: 0%, 0.5% and 1% of annatto concentrate in the diet, with blood collected from these animals in the prepartum, postpartum and weaning of the lambs. , processed to obtain washed red blood cells and blood plasma and performed analyzes of concentration of FRAP, TBAR's, hydrogen peroxide and reduced thiols and the activity of the enzymes glutathione peroxidase, superoxide dismutase and catalase. In the confinement of the lambs, 32 male lambs were used, divided into 4 treatments: 0%, 0.25%, 0.50% and 0.75% of annatto concentrate added to the feed provided. After reaching 42 kg of live weight, the animals were slaughtered and the portion of the Longissimus lumborum muscle was collected for analysis, where the meat was divided into 3 cm pieces and placed on trays, covered by plastic film, and lipid oxidation analysis was performed (TBAR's) and FRAP on days 0, 3, 7 and 14 of shelf life, concomitantly a subjective evaluation of consumers was carried out, where every 4 days they evaluated the acceptance of the samples by the color of the meat. With the studies it was concluded that the use of annatto concentrate in the diet of sheep can reduce oxidative stress in ewes at different reproductive stages and manages to control the lipid oxidation of the meat until the 7th day of shelf life, functioning as an antioxidant agent. |
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2023 |
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2023-09-25 |
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2024-10-10T17:10:28Z |
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Souza, Leticia Jalloul Guimarães de. Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos. 2023. 85f. Tese (Doutorado em Fisiopatologia e Saúde Animal) - Universidade do Oeste Paulista, Presidente Prudente. |
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Souza, Leticia Jalloul Guimarães de. Potencial antioxidante do urucum (bixa orellana) na dieta de ovinos. 2023. 85f. Tese (Doutorado em Fisiopatologia e Saúde Animal) - Universidade do Oeste Paulista, Presidente Prudente. |
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