Identificação da Variabilidade genética de Brachiaria ruziziensis por marcadores molecular

• Ano de defesa: 2009
• Autor(a) principal: Guaberto, Luciana Machado
• Orientador(a): Machado Neto, Nelson Barbosa
• Banca de defesa: Poletine, Juliana Parisotto , Silva, Matheus Gustavo da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Oeste Paulista
• Programa de Pós-Graduação: Mestrado em Agronomia
• Departamento: Ciências Agrárias
• País: BR
• Palavras-chave em Português: RAPD; Diversidade; Pastagem
• Palavras-chave em Inglês: RAPD; diversity; Pasture
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
• Link de acesso: http://bdtd.unoeste.br:8080/tede/handle/tede/528

Knowledge of the genetic variability of Brachiaria ruziziensis is required before you start a breeding program. This study investigated the genetic variability of 37 samples of Brachiaria ruziziensis, from the collection held by EMBRAPA Dairy Cattle by molecular markers and long primers. The DNA was extracted from leaves by CTAB method, we used 14 decamer primers and primers 9 long. The data were used for analysis of molecular variance (AMOVA) using the squared distances (Excoffier et al., 1992) using the program Arlequin (Excoffier et al., 2006). We found variation between species of 47.42% and 36.07% for RAPD primers and long, respectively and within the species observed variation of 52.58% and 63.93%. A dendrogram was constructed with the UPGMA clustering algorithm (Unweighted Pair-Group Method using an Arithmetic Average) using this analysis to the program NTSYS 2.0 (ROHLF 1998). Both markers were able to group the samples where genotypes 1 to 5 the most similar to each other. RAPD using primers short and long primers are effective to estimate the variability in Brachiaria ruziziensis.