Avaliação do dano genético no uso repetido de anestésicos locais: um estudo experimental em ratos

• Ano de defesa: 2013
• Autor(a) principal: Oliveira, Mariliza Casanova de
• Orientador(a): Nai, Gisele Alborghetti
• Banca de defesa: Nogueira, Rosa Maria Barilli , Santos, Paulo Sérgio da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Oeste Paulista
• Programa de Pós-Graduação: Mestrado em Ciência Animal
• Departamento: Ciências Agrárias
• País: BR
• Palavras-chave em Português: Anestesia Local; Genotoxicidade; Testes de Mutagenicidade; Testes para micronúcleos
• Palavras-chave em Inglês: Anesthesia, Local; Genotoxicity; Mutagenicity Tests; Micronucleus Tests
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://bdtd.unoeste.br:8080/tede/handle/tede/685

Studies with some local anesthetics showed that these can cause genetic damage. But local anesthetics has not been tested against the genotoxicity of repeated use. Objective: The aim of this study was to evaluate the genotoxic potential of local anesthetics with single dose and repeated, through the micronucleus test. Methods: We used 80 Wistar rats, male, 12 weeks old, divided into 6 groups: A - 16 rats received lidocaine hydrochloride intraperitoneally (4.4 mg / kg), B - 16 rats received 3% mepivacaine intraperitoneally (4.4 mg / kg), C - 16 rats received 4% articaine intraperitoneally (7.0 mg / kg) D - 16 rats received prilocaine 3% intraperitoneally (6.0 mg / kg) E - 08 rats received cyclophosphamide in single subcutaneous dose (50mg/kg) (positive control group) F - 08 rats received 0.5 ml of saline intraperitoneally (negative control group). Eight rats from Group A, B, C and D received the dose of anesthetic only once at the first day of the experiment and the remainder were dosed daily for five days. The Kruskal-Wallis test followed by a multiple comparisons Student-Newman-Keuls test was used for statistical analysis. Results: The median of micronuclei in the lidocaine group exposed for 1 day was 1.00 and by 5 days was 0.50, mepivacaine for 1 day and for 5 days was 1.00, articaine for 1 day was 1.00 and for 5 days 0.00, prilocaine for 1 day and 5 days was 0.00, cyclophosphamide was 10.00 and the negative control group exposed for 1 day was 1.00 and exposed for 5 days was 0.00 (p<0.0001). There was a statistical difference between the number of micronuclei in relation to the positive control group and all local anesthetics studied (related to the anesthetic type and duration of exposure) (p=0.0001) but not for the negative control group (p>0.05). Conclusion: There was no increase in micronuclei frequency in relation to exposure to 1 or 5 days in all local anesthetics evaluated.