Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco

Detalhes bibliográficos
Ano de defesa: 2010
Autor(a) principal: FREITAS, Soraya Farias de Andrade de lattes
Orientador(a): BARBOSA, Severino Benone Paes
Banca de defesa: GOMES FILHO, Manoel Adrião, SANTORO, Clebe Régis, MONARDES, Humberto Gonzalo
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural de Pernambuco
Programa de Pós-Graduação: Programa de Pós-Graduação em Zootecnia
Departamento: Departamento de Zootecnia
País: Brasil
Palavras-chave em Português:
Bovino
Girolando
Proteína
Leite
Palavras-chave em Inglês:
Milk
Protein
Área do conhecimento CNPq:
CIENCIAS AGRARIAS::ZOOTECNIA
Link de acesso: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6918
Resumo: The aim of the present work was to detect the genetic polymorphism of the β-Lactoglobulin (β-Lg) in different groups of Holstein-Gir breed cattle. There was collected 165 animals’ blood samples from the experimental farms of the Instituto Agronômico de Pernambuco (Itambé e Arcoverde) and from a private farm located in Zona da Mata Sul in the state of Pernambuco. The genetic groups were 1/2, 3/4, 5/8 Holstein and 5/8 H 1st, 2nd, 3rd and 4th bi-crossbred generation. For the identification of genotypes, a located fragment was amplified in the areas of exons II and III of β-Lg gene using the PCR technique. The result of the amplification was submitted to the PCR-RFLP analysis to digest the fragment by HaeIII endonuclease and it produced fragments of 400, 220, 190, and 151pb molecular weight for all animals, equivalent to the heterozygous genotype (100% AB) of β-Lg. The Pearson's chi-square test (2)and considering the observed and expected genotypic frequencies of the genotypes β-Lg. Was verified that frequences differed significantly at the level tested (P<0,05), indicating that the samples studied was not in Hardy-Weinberg equilibrium.
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spelling BARBOSA, Severino Benone PaesGOMES FILHO, Manoel AdriãoSANTORO, Clebe RégisMONARDES, Humberto Gonzalohttp://lattes.cnpq.br/1752452943678670FREITAS, Soraya Farias de Andrade de2017-05-18T13:55:24Z2010-02-22FREITAS, Soraya Farias de Andrade de. Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco. 2010. 61 f. Dissertação (Programa de Pós-Graduação em Zootecnia) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6918The aim of the present work was to detect the genetic polymorphism of the β-Lactoglobulin (β-Lg) in different groups of Holstein-Gir breed cattle. There was collected 165 animals’ blood samples from the experimental farms of the Instituto Agronômico de Pernambuco (Itambé e Arcoverde) and from a private farm located in Zona da Mata Sul in the state of Pernambuco. The genetic groups were 1/2, 3/4, 5/8 Holstein and 5/8 H 1st, 2nd, 3rd and 4th bi-crossbred generation. For the identification of genotypes, a located fragment was amplified in the areas of exons II and III of β-Lg gene using the PCR technique. The result of the amplification was submitted to the PCR-RFLP analysis to digest the fragment by HaeIII endonuclease and it produced fragments of 400, 220, 190, and 151pb molecular weight for all animals, equivalent to the heterozygous genotype (100% AB) of β-Lg. The Pearson's chi-square test (2)and considering the observed and expected genotypic frequencies of the genotypes β-Lg. Was verified that frequences differed significantly at the level tested (P<0,05), indicating that the samples studied was not in Hardy-Weinberg equilibrium.O objetivo do presente estudo foi detectar o polimorfismo genético da β-Lactoglobulina (β-Lg) em diferentes grupos genéticos Holando-Gir. Foram coletadas 165 amostras de sangue de animais provenientes das fazendas experimentais do Instituto Agronômico de Pernambuco (Itambé e Arcoverde) e de uma propriedade particular, localizada na Zona da Mata Sul do estado. Os grupos genéticos encontrados foram 1/2, 3/4, 5/8 Holandês e 5/8 H bimestiço de 1ª, 2ª, 3ª, e 4ª geração. Para identificação dos genótipos, amplificou-se um fragmento localizado nas regiões dos éxons II e III do gene da β-Lg pela técnica de PCR. O produto da amplificação foi submetido à técnica de PCRRFLP para digestão do fragmento pela endonuclease HaeIII, e gerou fragmentos de peso molecular de 400, 220, 190, e 151pb para todos os indivíduos, o equivalente ao genótipo heterozigoto (100% AB) da β-Lg. Deacordo com o teste de qui-quadrado (2), considerando as frequências genotípicas observadas e esperadas da β-Lg, verificou-se que estas diferiram significativamente (P<0,05), o que indica que o locus em estudo da amostra populacional não se encontra em equilíbrio genético pela lei de Hardy- Weinberg.Submitted by (lucia.rodrigues@ufrpe.br) on 2017-05-18T13:55:24Z No. of bitstreams: 1 Soraya Farias de Andrade de Freitas.pdf: 776131 bytes, checksum: 1494bcef1d2ef794e6ccad6a07798f18 (MD5)Made available in DSpace on 2017-05-18T13:55:24Z (GMT). No. of bitstreams: 1 Soraya Farias de Andrade de Freitas.pdf: 776131 bytes, checksum: 1494bcef1d2ef794e6ccad6a07798f18 (MD5) Previous issue date: 2010-02-22application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em ZootecniaUFRPEBrasilDepartamento de ZootecniaBovinoGirolandoProteínaLeiteMilkProteinCIENCIAS AGRARIAS::ZOOTECNIAEstudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambucoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3881065194686295060600600600-76856541506829724321346858981270845602info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6918/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALSoraya Farias de Andrade de Freitas.pdfSoraya Farias de Andrade de Freitas.pdfapplication/pdf776131http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6918/2/Soraya+Farias+de+Andrade+de+Freitas.pdf1494bcef1d2ef794e6ccad6a07798f18MD52tede2/69182017-05-18 10:55:24.594oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2017-05-18T13:55:24Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
title Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
spellingShingle Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
FREITAS, Soraya Farias de Andrade de
Bovino
Girolando
Proteína
Leite
Milk
Protein
CIENCIAS AGRARIAS::ZOOTECNIA
title_short Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
title_full Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
title_fullStr Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
title_full_unstemmed Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
title_sort Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco
author FREITAS, Soraya Farias de Andrade de
author_facet FREITAS, Soraya Farias de Andrade de
author_role author
dc.contributor.advisor1.fl_str_mv BARBOSA, Severino Benone Paes
dc.contributor.referee1.fl_str_mv GOMES FILHO, Manoel Adrião
dc.contributor.referee2.fl_str_mv SANTORO, Clebe Régis
dc.contributor.referee3.fl_str_mv MONARDES, Humberto Gonzalo
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1752452943678670
dc.contributor.author.fl_str_mv FREITAS, Soraya Farias de Andrade de
contributor_str_mv BARBOSA, Severino Benone Paes
GOMES FILHO, Manoel Adrião
SANTORO, Clebe Régis
MONARDES, Humberto Gonzalo
dc.subject.por.fl_str_mv Bovino
Girolando
Proteína
Leite
topic Bovino
Girolando
Proteína
Leite
Milk
Protein
CIENCIAS AGRARIAS::ZOOTECNIA
dc.subject.eng.fl_str_mv Milk
Protein
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::ZOOTECNIA
description The aim of the present work was to detect the genetic polymorphism of the β-Lactoglobulin (β-Lg) in different groups of Holstein-Gir breed cattle. There was collected 165 animals’ blood samples from the experimental farms of the Instituto Agronômico de Pernambuco (Itambé e Arcoverde) and from a private farm located in Zona da Mata Sul in the state of Pernambuco. The genetic groups were 1/2, 3/4, 5/8 Holstein and 5/8 H 1st, 2nd, 3rd and 4th bi-crossbred generation. For the identification of genotypes, a located fragment was amplified in the areas of exons II and III of β-Lg gene using the PCR technique. The result of the amplification was submitted to the PCR-RFLP analysis to digest the fragment by HaeIII endonuclease and it produced fragments of 400, 220, 190, and 151pb molecular weight for all animals, equivalent to the heterozygous genotype (100% AB) of β-Lg. The Pearson's chi-square test (2)and considering the observed and expected genotypic frequencies of the genotypes β-Lg. Was verified that frequences differed significantly at the level tested (P<0,05), indicating that the samples studied was not in Hardy-Weinberg equilibrium.
publishDate 2010
dc.date.issued.fl_str_mv 2010-02-22
dc.date.accessioned.fl_str_mv 2017-05-18T13:55:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv FREITAS, Soraya Farias de Andrade de. Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco. 2010. 61 f. Dissertação (Programa de Pós-Graduação em Zootecnia) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6918
identifier_str_mv FREITAS, Soraya Farias de Andrade de. Estudo do polimorfismo genético da beta-lactoglobulina em bovinos Holando-Gir no Estado de Pernambuco. 2010. 61 f. Dissertação (Programa de Pós-Graduação em Zootecnia) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6918
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dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Zootecnia
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Zootecnia
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
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