Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro
Ano de defesa: | 2011 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Departamento de Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6338 |
Resumo: | The Opuntia stricta Haw, cactus of great economic importance as forage in northeastern Brazil, was subjected to different in vitro systems and their performance was assessed by physiological and biochemical variables. We established three different cropping systems: temporary immersion system (TIS), with low frequency of immersion (immersion of one minute every 48 hours) and high-frequency TIS (immersion for five minutes every three hours); static culture in semi-solid medium (6.0 g.L-1 agar); static culture in liquid medium. The middle for inducing proliferation in vitro consisted of Murashige and Skoog (1962) salts and vitamins plus 30.0 g.L-1 sucrose and 0.0, 0.25, 0.5 or 1.0 mg.L-1 6-benzilaminpurine (BAP). Cultures were maintained in a growth room with 25 ± 2 º C under cool white light (40 mol.m-2.s-1), with 16 hours photoperiod. The experimental design was completely randomized factorial arrangement with 4 x 4 (cropping systems x BAP concentrations in the middle). After cultivation in vitro shoots of each treatment were acclimatized. Biochemical and biometrics analysis were performed on the material in vitro and biometrics on the acclimatized material. The static culture on solid medium, provided more stability since the multiplication by the acclimatization of the seedlings, while the systems with liquid presented limitations in some stages of micropropagation. The vitrification and the presence of betacyanin can be used as quality indicators physiological of the Elephant Ear Mexicana and its potential for acclimatization. |
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CAMARA, Terezinha de Jesus RangelULISSES, CláudiaWANDERLEY, Lília GomesLIMA, Vera Lúcia Arroxelas Galvão deCARVALHO, Josabete Salgueiro BezerraMEDEIROS, Emmanuel Cabral2017-02-14T14:53:53Z2011-02-16MEDEIROS, Emmanuel Cabral. Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro. 2011. 80 f. Dissertação (Programa de Pós-Graduação em Química) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6338The Opuntia stricta Haw, cactus of great economic importance as forage in northeastern Brazil, was subjected to different in vitro systems and their performance was assessed by physiological and biochemical variables. We established three different cropping systems: temporary immersion system (TIS), with low frequency of immersion (immersion of one minute every 48 hours) and high-frequency TIS (immersion for five minutes every three hours); static culture in semi-solid medium (6.0 g.L-1 agar); static culture in liquid medium. The middle for inducing proliferation in vitro consisted of Murashige and Skoog (1962) salts and vitamins plus 30.0 g.L-1 sucrose and 0.0, 0.25, 0.5 or 1.0 mg.L-1 6-benzilaminpurine (BAP). Cultures were maintained in a growth room with 25 ± 2 º C under cool white light (40 mol.m-2.s-1), with 16 hours photoperiod. The experimental design was completely randomized factorial arrangement with 4 x 4 (cropping systems x BAP concentrations in the middle). After cultivation in vitro shoots of each treatment were acclimatized. Biochemical and biometrics analysis were performed on the material in vitro and biometrics on the acclimatized material. The static culture on solid medium, provided more stability since the multiplication by the acclimatization of the seedlings, while the systems with liquid presented limitations in some stages of micropropagation. The vitrification and the presence of betacyanin can be used as quality indicators physiological of the Elephant Ear Mexicana and its potential for acclimatization.A Opuntia stricta Haw, cactácea de grande importância econômica como forrageira na região Nordeste do Brasil, foi submetida a distintos sistemas de cultivo in vitro e seu desempenho foi avaliado mediante variáveis fisiológicas e bioquímicas. Foram estabelecidos quatro diferentes sistemas de cultivo: sistema de imersão temporária (SIT), com baixa freqüência de imersões (imersão de um minuto a cada 48 horas); alta freqüência (imersão de cinco minutos a cada três horas); cultivo estático em meio semi-sólido (6,0 g.L-1 de ágar); cultivo estático em meio líquido. Os meios para indução de multiplicação in vitro constaram dos sais e vitaminas de Murashige e Skoog (1962) acrescidos de 30,0 g.L-1 de sacarose e 0,0; 0,25; 0,5 ou 1,0 mg.L-1 de 6 benzilaminopurina (BAP). As culturas foram mantidas em sala de crescimento com 25±2ºC, sob luz branca fria (40 μmol.m-2.s-1), com 16 horas de fotoperíodo. O desenho experimental foi o inteiramente casualizado com arranjo fatorial 4 x 4 (sistemas de cultivo x concentrações de BAP no meio). Após o cultivo in vitro, brotações de cada tratamento foram aclimatizadas. Análises bioquímicas e biométricas foram realizadas no material in vitro e biométricas no material aclimatizado. O cultivo estático em meio sólido, proporcionou mais estabilidade desde a multiplicação até a aclimatização das mudas, enquanto os sistemas com meio líquido apresentaram limitações em alguma das fases da micropropagação. A hiperidricidade e a presença de betacianina podem ser usadas como indicadores da qualidade fisiológica das mudas micropropagadas da Orelha de Elefante Mexicana e do seu potencial para aclimatização.Submitted by (lucia.rodrigues@ufrpe.br) on 2017-02-14T14:53:53Z No. of bitstreams: 1 Emanoel Cabral de Medeiros.pdf: 910531 bytes, checksum: a49d49dbcc2fa0475cf92eaff69ab023 (MD5)Made available in DSpace on 2017-02-14T14:53:53Z (GMT). No. of bitstreams: 1 Emanoel Cabral de Medeiros.pdf: 910531 bytes, checksum: a49d49dbcc2fa0475cf92eaff69ab023 (MD5) Previous issue date: 2011-02-16Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em QuímicaUFRPEBrasilDepartamento de QuímicaMorphogenesisPhysiologicCactus pearPalma forrageiraHiperidricidadeBiorreatorEstresse oxidativoMorfogêneseOxidative stressBioreactorVitrificationQuímicaCIENCIAS EXATAS E DA TERRA::QUIMICAAspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitroPhysiologic and biochemical aspects of the cactus pear Opuntia stricta Haw in diferent in vitro cropping systemsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1435648362225100898600600600600380641605545709103015717003253031171952075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6338/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALEmanoel Cabral de Medeiros.pdfEmanoel Cabral de Medeiros.pdfapplication/pdf910531http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/6338/2/Emanoel+Cabral+de+Medeiros.pdfa49d49dbcc2fa0475cf92eaff69ab023MD52tede2/63382024-03-26 15:15:22.835oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-03-26T18:15:22Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro |
dc.title.alternative.por.fl_str_mv |
Physiologic and biochemical aspects of the cactus pear Opuntia stricta Haw in diferent in vitro cropping systems |
title |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro |
spellingShingle |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro MEDEIROS, Emmanuel Cabral Morphogenesis Physiologic Cactus pear Palma forrageira Hiperidricidade Biorreator Estresse oxidativo Morfogênese Oxidative stress Bioreactor Vitrification Química CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro |
title_full |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro |
title_fullStr |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro |
title_full_unstemmed |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro |
title_sort |
Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro |
author |
MEDEIROS, Emmanuel Cabral |
author_facet |
MEDEIROS, Emmanuel Cabral |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
CAMARA, Terezinha de Jesus Rangel |
dc.contributor.advisor-co1.fl_str_mv |
ULISSES, Cláudia |
dc.contributor.referee1.fl_str_mv |
WANDERLEY, Lília Gomes |
dc.contributor.referee2.fl_str_mv |
LIMA, Vera Lúcia Arroxelas Galvão de |
dc.contributor.referee3.fl_str_mv |
CARVALHO, Josabete Salgueiro Bezerra |
dc.contributor.author.fl_str_mv |
MEDEIROS, Emmanuel Cabral |
contributor_str_mv |
CAMARA, Terezinha de Jesus Rangel ULISSES, Cláudia WANDERLEY, Lília Gomes LIMA, Vera Lúcia Arroxelas Galvão de CARVALHO, Josabete Salgueiro Bezerra |
dc.subject.por.fl_str_mv |
Morphogenesis Physiologic Cactus pear Palma forrageira Hiperidricidade Biorreator Estresse oxidativo Morfogênese Oxidative stress Bioreactor Vitrification Química |
topic |
Morphogenesis Physiologic Cactus pear Palma forrageira Hiperidricidade Biorreator Estresse oxidativo Morfogênese Oxidative stress Bioreactor Vitrification Química CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The Opuntia stricta Haw, cactus of great economic importance as forage in northeastern Brazil, was subjected to different in vitro systems and their performance was assessed by physiological and biochemical variables. We established three different cropping systems: temporary immersion system (TIS), with low frequency of immersion (immersion of one minute every 48 hours) and high-frequency TIS (immersion for five minutes every three hours); static culture in semi-solid medium (6.0 g.L-1 agar); static culture in liquid medium. The middle for inducing proliferation in vitro consisted of Murashige and Skoog (1962) salts and vitamins plus 30.0 g.L-1 sucrose and 0.0, 0.25, 0.5 or 1.0 mg.L-1 6-benzilaminpurine (BAP). Cultures were maintained in a growth room with 25 ± 2 º C under cool white light (40 mol.m-2.s-1), with 16 hours photoperiod. The experimental design was completely randomized factorial arrangement with 4 x 4 (cropping systems x BAP concentrations in the middle). After cultivation in vitro shoots of each treatment were acclimatized. Biochemical and biometrics analysis were performed on the material in vitro and biometrics on the acclimatized material. The static culture on solid medium, provided more stability since the multiplication by the acclimatization of the seedlings, while the systems with liquid presented limitations in some stages of micropropagation. The vitrification and the presence of betacyanin can be used as quality indicators physiological of the Elephant Ear Mexicana and its potential for acclimatization. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-16 |
dc.date.accessioned.fl_str_mv |
2017-02-14T14:53:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
MEDEIROS, Emmanuel Cabral. Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro. 2011. 80 f. Dissertação (Programa de Pós-Graduação em Química) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6338 |
identifier_str_mv |
MEDEIROS, Emmanuel Cabral. Aspectos bioquímicos e fisiológicos da palma forrageira Opuntia stricta Haw sob distintos sistemas de cultivo in vitro. 2011. 80 f. Dissertação (Programa de Pós-Graduação em Química) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6338 |
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