Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Mada, Edson Yoshihiro lattes
Orientador(a): Matsumoto, Mariza Akemi lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade do Sagrado Coração
Programa de Pós-Graduação: Biologia Oral
Departamento: Ciências da Saúde e Biológicas
País: Brasil
Palavras-chave em Português:
Bifosfonatos
Osteonecrose
Chá verde
Ratos
Palavras-chave em Inglês:
Biphosphonates
Osteonecrosis
Green tea
Rats
Área do conhecimento CNPq:
CIENCIAS DA SAUDE::ODONTOLOGIA
Link de acesso: http://tede2.usc.br:8080/jspui/handle/tede/321
Resumo: Bisphosphonate (Bp) induced osteonecrosis of the jaws is considered as a significant complication when the need for surgical interventions in the oral cavity, the prevention and treatment are not yet well established. Thus, this study aimed to analyze the effect of 1% GT in the repair of dental sockets in animals treated with intravenous (IV) bisphosphonate. Sixty Wistar male rats were set in four groups according to the treatment: C group - control, animals received IV injection of 0.9% saline; GT group - animals received an intravenous injection of 0.9% saline + 1% GT in drinking water; ZA Group - treated with IV injection of zoledronic acid (ZA) + GT Group - treated with intravenous injection of ZA and 1% GT. The drug was administered at a dose of 0.035 mg/kg every two weeks. Eight weeks after the start of administration of ZA, extractions of the upper right molar teeth from all groups were obtained, when 1% GT was taken orally as the only source of water to animals of GT and ZA+GT groups. After periods of 7, 14 and 28 days of dental extractions, the animals were euthanized and specimens removed for histological and OPG, RANK, and TRAP immunohistochemistry analysis. Dental sockets from C group repaired in accordance with normal morphology and chronology. The dental socket of the experimental groups had similar morphology of repair, with persistence of the bone surface exposure over the periods associated with the accession of bacterial biofilm. The GT group showed moderate osteoclastic activity and discreet osteogenesis, while in ZA and ZA+GT groups the wound healing had not occurred up to 28 days with clear installation of osteonecrosis of the maxilla. Attention was given to TRAP immunolabeling, which presented osteoclasts in granulation tissue of ZA and ZA+GT groups. Thus, it was concluded that the intake of green tea did not improve the repair of the dental sockets compared to the control group. Similarly, also did not contribute significantly to repair the association of ZA observing the same histological characteristics when compared to animals receiving ZA alone
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spelling Matsumoto, Mariza Akemi137.278.308-3213.501.944http://lattes.cnpq.br/8621510886887389057.739.458-4315.431.204http://lattes.cnpq.br/8302163178704802Mada, Edson Yoshihiro2017-03-31T00:08:18Z2015-02-23MADA, Edson Yoshihiro. Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato. 2015. 52 f. Tese (Doutorado em Biologia Oral - Área de concentração: Biologia Oral) - Universidade do Sagrado Coração, Bauru, 2015 .http://tede2.usc.br:8080/jspui/handle/tede/321Bisphosphonate (Bp) induced osteonecrosis of the jaws is considered as a significant complication when the need for surgical interventions in the oral cavity, the prevention and treatment are not yet well established. Thus, this study aimed to analyze the effect of 1% GT in the repair of dental sockets in animals treated with intravenous (IV) bisphosphonate. Sixty Wistar male rats were set in four groups according to the treatment: C group - control, animals received IV injection of 0.9% saline; GT group - animals received an intravenous injection of 0.9% saline + 1% GT in drinking water; ZA Group - treated with IV injection of zoledronic acid (ZA) + GT Group - treated with intravenous injection of ZA and 1% GT. The drug was administered at a dose of 0.035 mg/kg every two weeks. Eight weeks after the start of administration of ZA, extractions of the upper right molar teeth from all groups were obtained, when 1% GT was taken orally as the only source of water to animals of GT and ZA+GT groups. After periods of 7, 14 and 28 days of dental extractions, the animals were euthanized and specimens removed for histological and OPG, RANK, and TRAP immunohistochemistry analysis. Dental sockets from C group repaired in accordance with normal morphology and chronology. The dental socket of the experimental groups had similar morphology of repair, with persistence of the bone surface exposure over the periods associated with the accession of bacterial biofilm. The GT group showed moderate osteoclastic activity and discreet osteogenesis, while in ZA and ZA+GT groups the wound healing had not occurred up to 28 days with clear installation of osteonecrosis of the maxilla. Attention was given to TRAP immunolabeling, which presented osteoclasts in granulation tissue of ZA and ZA+GT groups. Thus, it was concluded that the intake of green tea did not improve the repair of the dental sockets compared to the control group. Similarly, also did not contribute significantly to repair the association of ZA observing the same histological characteristics when compared to animals receiving ZA aloneA osteonecrose dos maxilares induzidas por bifosfonatos (BFs) nitrogenados é considerada uma complicação importante quando da necessidade de intervenções cirúrgicas na cavidade bucal, cuja prevenção e tratamento ainda não estão bem estabelecidos. Deste modo, o presente estudo teve por objetivo analisar o efeito do chá verde (CV) a 1% no reparo de alvéolos dentários em animais tratados com BFs, endovenoso (EV). Sessenta ratos machos adultos compuseram quatro grupos, de acordo com o tratamento: Grupo C – Controle, animais tratados com soro fisiológico 0,9% (SF) via (EV); Grupo CV – tratados com CV a 1% na água de beber; Grupo ZL - tratados com ácido zoledrônico (ZL) via EV, Grupo ZL+CV - tratados com AZ via EV e administração de CV a 1% na água de beber. A droga foi administrada na dose de 0,035 mg/Kg via EV a cada duas semanas. Após 8 semanas do início da administração do ácido zoledrônico, foram realizadas as extrações dos dentes molares superiores do lado direito dos animais de todos os grupos, quando se iniciou a administração do CV a 1% aos animais dos grupos CV e ZL+CV. Após os períodos de 7, 14 e 28 dias das extrações dentárias os animais foram eutanasiados e os espécimes submetidos à análise microscópica morfológica e imunoistoquímica para OPG, RANKL e TRAP. Os alvéolos do Grupo C repararam de acordo com morfologia e cronologia normais. Os dos grupos experimentais apresentaram padrão morfológico de reparo similar, com persistência de exposição da superfície óssea no decorrer dos períodos, associado à adesão de biofilme bacteriano. O Grupo CV apresentou moderada atividade osteoclástica e discreta osteogênese, enquanto que nos Grupos ZL e ZL+CV o reparo alveolar não havia ocorrido até os 28 dias, com evidente instalação do quadro de osteonecrose dos maxilares. Chamou a atenção a imunomarcação para TRAP, evidenciando células osteoclásticas dispersas pelo tecido de granulação nos Grupos ZL e ZL+CV. Concluiu-se que a ingestão do CV não beneficiou o reparo dos alvéolos em comparação ao grupo controle. Do mesmo modo, também não contribuiu de maneira importante para o reparo na associação do ácido zoledrônico observando-se as mesmas características histológicas quando comparado aos animais que receberam o ZL isoladamenteSubmitted by Laudeceia Machado (laudeceia.machado@usc.br) on 2017-03-31T00:08:18Z No. of bitstreams: 1 Efeito do cha verde no processo de reparo alveolar de ratos tratados com bifosfonato (196664).pdf: 5837371 bytes, checksum: fb8d452ffd0689efafc2979262ef8b70 (MD5)Made available in DSpace on 2017-03-31T00:08:18Z (GMT). No. of bitstreams: 1 Efeito do cha verde no processo de reparo alveolar de ratos tratados com bifosfonato (196664).pdf: 5837371 bytes, checksum: fb8d452ffd0689efafc2979262ef8b70 (MD5) Previous issue date: 2015-02-23application/pdfporUniversidade do Sagrado CoraçãoBiologia OralUSCBrasilCiências da Saúde e BiológicasBifosfonatosOsteonecroseChá verdeRatosBiphosphonatesOsteonecrosisGreen teaRatsCIENCIAS DA SAUDE::ODONTOLOGIAEfeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonatoEffect of green tea on the alveolar repair process of bisphosphonate-treated ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8043334104093724546600600600-753428887677182963-2070498469879244349info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da USCinstname:Universidade do Sagrado Coração (USC)instacron:USCLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/321/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALEfeito do cha verde no processo de reparo alveolar de ratos tratados com bifosfonato (196664).pdfEfeito do cha verde no processo de reparo alveolar de ratos tratados com bifosfonato (196664).pdfapplication/pdf5837371http://localhost:8080/tede/bitstream/tede/321/2/Efeito+do+cha+verde+no+processo+de+reparo+alveolar+de+ratos+tratados+com+bifosfonato+%28196664%29.pdffb8d452ffd0689efafc2979262ef8b70MD52tede/3212017-03-30 21:08:18.52oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.usc.br:8443/http://tede2.usc.br:8080/oai/requestbiblicorjesu@unisagrado.edu.br||normalizacao@usc.bropendoar:2017-03-31T00:08:18Biblioteca Digital de Teses e Dissertações da USC - Universidade do Sagrado Coração (USC)false
dc.title.eng.fl_str_mv Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
dc.title.alternative.eng.fl_str_mv Effect of green tea on the alveolar repair process of bisphosphonate-treated rats
title Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
spellingShingle Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
Mada, Edson Yoshihiro
Bifosfonatos
Osteonecrose
Chá verde
Ratos
Biphosphonates
Osteonecrosis
Green tea
Rats
CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
title_full Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
title_fullStr Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
title_full_unstemmed Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
title_sort Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato
author Mada, Edson Yoshihiro
author_facet Mada, Edson Yoshihiro
author_role author
dc.contributor.advisor1.fl_str_mv Matsumoto, Mariza Akemi
dc.contributor.advisor1ID.fl_str_mv 137.278.308-32
13.501.944
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8621510886887389
dc.contributor.authorID.fl_str_mv 057.739.458-43
15.431.204
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8302163178704802
dc.contributor.author.fl_str_mv Mada, Edson Yoshihiro
contributor_str_mv Matsumoto, Mariza Akemi
dc.subject.por.fl_str_mv Bifosfonatos
Osteonecrose
Chá verde
Ratos
topic Bifosfonatos
Osteonecrose
Chá verde
Ratos
Biphosphonates
Osteonecrosis
Green tea
Rats
CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv Biphosphonates
Osteonecrosis
Green tea
Rats
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE::ODONTOLOGIA
description Bisphosphonate (Bp) induced osteonecrosis of the jaws is considered as a significant complication when the need for surgical interventions in the oral cavity, the prevention and treatment are not yet well established. Thus, this study aimed to analyze the effect of 1% GT in the repair of dental sockets in animals treated with intravenous (IV) bisphosphonate. Sixty Wistar male rats were set in four groups according to the treatment: C group - control, animals received IV injection of 0.9% saline; GT group - animals received an intravenous injection of 0.9% saline + 1% GT in drinking water; ZA Group - treated with IV injection of zoledronic acid (ZA) + GT Group - treated with intravenous injection of ZA and 1% GT. The drug was administered at a dose of 0.035 mg/kg every two weeks. Eight weeks after the start of administration of ZA, extractions of the upper right molar teeth from all groups were obtained, when 1% GT was taken orally as the only source of water to animals of GT and ZA+GT groups. After periods of 7, 14 and 28 days of dental extractions, the animals were euthanized and specimens removed for histological and OPG, RANK, and TRAP immunohistochemistry analysis. Dental sockets from C group repaired in accordance with normal morphology and chronology. The dental socket of the experimental groups had similar morphology of repair, with persistence of the bone surface exposure over the periods associated with the accession of bacterial biofilm. The GT group showed moderate osteoclastic activity and discreet osteogenesis, while in ZA and ZA+GT groups the wound healing had not occurred up to 28 days with clear installation of osteonecrosis of the maxilla. Attention was given to TRAP immunolabeling, which presented osteoclasts in granulation tissue of ZA and ZA+GT groups. Thus, it was concluded that the intake of green tea did not improve the repair of the dental sockets compared to the control group. Similarly, also did not contribute significantly to repair the association of ZA observing the same histological characteristics when compared to animals receiving ZA alone
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-23
dc.date.accessioned.fl_str_mv 2017-03-31T00:08:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
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dc.identifier.citation.fl_str_mv MADA, Edson Yoshihiro. Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato. 2015. 52 f. Tese (Doutorado em Biologia Oral - Área de concentração: Biologia Oral) - Universidade do Sagrado Coração, Bauru, 2015 .
dc.identifier.uri.fl_str_mv http://tede2.usc.br:8080/jspui/handle/tede/321
identifier_str_mv MADA, Edson Yoshihiro. Efeito do chá verde no processo de reparo alveolar de ratos tratados com bifosfonato. 2015. 52 f. Tese (Doutorado em Biologia Oral - Área de concentração: Biologia Oral) - Universidade do Sagrado Coração, Bauru, 2015 .
url http://tede2.usc.br:8080/jspui/handle/tede/321
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dc.publisher.none.fl_str_mv Universidade do Sagrado Coração
dc.publisher.program.fl_str_mv Biologia Oral
dc.publisher.initials.fl_str_mv USC
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Ciências da Saúde e Biológicas
publisher.none.fl_str_mv Universidade do Sagrado Coração
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