Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil
Ano de defesa: | 2015 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | eng |
Instituição de defesa: |
Biblioteca Digitais de Teses e Dissertações da USP
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Programa de Pós-Graduação: |
Não Informado pela instituição
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Departamento: |
Não Informado pela instituição
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País: |
Não Informado pela instituição
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Palavras-chave em Português: | |
Link de acesso: | http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04032016-154921/ |
Resumo: | Infectious bronchitis virus (IBV) is the causative agent of an economically important disease of poultry. In Brazil this disease causes respiratory, renal and reproductive problems in birds of all ages, despite constant vaccination with the Massachusetts strain H120. This lack of immunological protection is known to be due the genetic variation in the spike glycoprotein of IBV, which is involved in host cell attachment, neutralization and the induction of protective immunity. Brazilian IBV variants resulting of this genetic variation are present since the 80s and this study aimed to epidemiologicaly analyze and molecularly characterize the existing variants during 2010-2015 and perform a bioinformatics analysis of the available sequences of IBV variants in a 40 year period. Of the 453 samples tested, 61.4% were positive for IBV and 75.9% of them were considered variants and were detected in birds of all ages, distributed in all five Brazilian regions. A fragment of 559-566 bp was obtained from 12 isolates, where BR-I was the predominant variant while only one isolate belonged to the BR-II genotype. Bioinformatics analysis of the sequences of 40 years of Brazilian IBV variants was performed and the ratio of non-synonymous substitutions per non-synonymous site (dn) to synonymous substitutions per synonymous site (ds) dN/dS was calculated. It revealed a predominance of codons with non-synonymous substitutions in the first third of the S1 gene and a dN/dS ratio of 0.6757, indicating that this portion of the gene was under negative selection. Additionally prediction of N-glycosilation sites showed that most of the BR-I variants (from 2003 to early 2014) present an extra site at animoacid position 20, while the newest ones lack this feature.Together these results suggest that IBV Brazilian variants had probably suffered drastic mutations in some points between the years 1983 to 2003 and after achieving an antigenic structure effective enough for invasion and replication in their hosts, the selection processes became silent. |
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Biblioteca Digital de Teses e Dissertações da USP |
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Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in BrazilDiversidade genética do vírus da bronquite infecciosa isolado de aves de produção no BrasilBR-IBR-IBR-IIBR-IIdN/dSdN/dSGlicoproteína da espículan-glicosilaçãon-glycosilationSpike glycoproteinInfectious bronchitis virus (IBV) is the causative agent of an economically important disease of poultry. In Brazil this disease causes respiratory, renal and reproductive problems in birds of all ages, despite constant vaccination with the Massachusetts strain H120. This lack of immunological protection is known to be due the genetic variation in the spike glycoprotein of IBV, which is involved in host cell attachment, neutralization and the induction of protective immunity. Brazilian IBV variants resulting of this genetic variation are present since the 80s and this study aimed to epidemiologicaly analyze and molecularly characterize the existing variants during 2010-2015 and perform a bioinformatics analysis of the available sequences of IBV variants in a 40 year period. Of the 453 samples tested, 61.4% were positive for IBV and 75.9% of them were considered variants and were detected in birds of all ages, distributed in all five Brazilian regions. A fragment of 559-566 bp was obtained from 12 isolates, where BR-I was the predominant variant while only one isolate belonged to the BR-II genotype. Bioinformatics analysis of the sequences of 40 years of Brazilian IBV variants was performed and the ratio of non-synonymous substitutions per non-synonymous site (dn) to synonymous substitutions per synonymous site (ds) dN/dS was calculated. It revealed a predominance of codons with non-synonymous substitutions in the first third of the S1 gene and a dN/dS ratio of 0.6757, indicating that this portion of the gene was under negative selection. Additionally prediction of N-glycosilation sites showed that most of the BR-I variants (from 2003 to early 2014) present an extra site at animoacid position 20, while the newest ones lack this feature.Together these results suggest that IBV Brazilian variants had probably suffered drastic mutations in some points between the years 1983 to 2003 and after achieving an antigenic structure effective enough for invasion and replication in their hosts, the selection processes became silent.O vírus da bronquite infecciosa das galinhas (IBV) é o agente causador de uma doença aviária economicamente importante. No Brasil, esta doença ocasiona problemas respiratórios, renais e reprodutivos em aves de todas as idades, apesar da vacinação constante com a cepa Massachusetts H120. Esta falha na proteção conferida pela vacina é ocasionada por mutações nos nucleotídeos do gene da glicoproteína da espícula, a qual está envolvida no processo de interação comas células do hospedeiro, a neutralização e a indução de imunidade protetora. As variantes brasileiras resultantes dessa mutação genética estão presentes desde os anos 80 e este estudo teve como objetivo analisar epidemiologicamente e caracterizar molecularmente os vírus variantes existentes durante 2010-2015 e realizar uma análise bioinformática das sequências disponíveis no GenBank em um período de 40 anos. Das 453 amostras analisadas, 61,4% foram positivas para IBV e 75,9% delas foram consideradas variantes e foram detectados em aves de todas as idades, distribuídos em todas as 5 regiões do Brasil. Um fragmento de 559-566 pb foi obtido a partir de 12 isolados, onde BR-I foi a variante predominante ao contrario que apenas um isolado pertencia ao genótipo BR-II. Análise bioinformática de 40 anos de variantes do IBV brasileiros revelou uma predominância de codões com as substituições não sinónimos no primeiro terço do gene S1 e uma relação dN / dS de 0,6757, indicando que esta porção do gene estava sob selecção negativa. Além disso a previsão de pontos de de N-glicosilação mostrou que a maioria das amostras variantes BR-I (entre o 2003 e início de 2014) apresentam um ponto adicional na posição 20, enquanto as variantes mais novas não apresentam esse ponto de nglicosilação. Estes resultados sugerem que as variantes brasileiras teriam sofrido mutações provavelmente drásticas em alguns pontos do genoma, entre os anos de 1983 a 2003 e depois de atingir uma estrutura antigênica eficaz o suficiente para a invasão e replicação em seus hospedeiros, o processo de seleção mudou para seleção negativa.Biblioteca Digitais de Teses e Dissertações da USPFerreira, Antônio José PiantinoChamorro, Claudia Carranza2015-12-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/10/10134/tde-04032016-154921/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-10-09T13:16:04Zoai:teses.usp.br:tde-04032016-154921Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-10-09T13:16:04Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil Diversidade genética do vírus da bronquite infecciosa isolado de aves de produção no Brasil |
title |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil |
spellingShingle |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil Chamorro, Claudia Carranza BR-I BR-I BR-II BR-II dN/dS dN/dS Glicoproteína da espícula n-glicosilação n-glycosilation Spike glycoprotein |
title_short |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil |
title_full |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil |
title_fullStr |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil |
title_full_unstemmed |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil |
title_sort |
Genetic diversity of avian coronavirus infectious bronchitis detected from commercial poultry in Brazil |
author |
Chamorro, Claudia Carranza |
author_facet |
Chamorro, Claudia Carranza |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ferreira, Antônio José Piantino |
dc.contributor.author.fl_str_mv |
Chamorro, Claudia Carranza |
dc.subject.por.fl_str_mv |
BR-I BR-I BR-II BR-II dN/dS dN/dS Glicoproteína da espícula n-glicosilação n-glycosilation Spike glycoprotein |
topic |
BR-I BR-I BR-II BR-II dN/dS dN/dS Glicoproteína da espícula n-glicosilação n-glycosilation Spike glycoprotein |
description |
Infectious bronchitis virus (IBV) is the causative agent of an economically important disease of poultry. In Brazil this disease causes respiratory, renal and reproductive problems in birds of all ages, despite constant vaccination with the Massachusetts strain H120. This lack of immunological protection is known to be due the genetic variation in the spike glycoprotein of IBV, which is involved in host cell attachment, neutralization and the induction of protective immunity. Brazilian IBV variants resulting of this genetic variation are present since the 80s and this study aimed to epidemiologicaly analyze and molecularly characterize the existing variants during 2010-2015 and perform a bioinformatics analysis of the available sequences of IBV variants in a 40 year period. Of the 453 samples tested, 61.4% were positive for IBV and 75.9% of them were considered variants and were detected in birds of all ages, distributed in all five Brazilian regions. A fragment of 559-566 bp was obtained from 12 isolates, where BR-I was the predominant variant while only one isolate belonged to the BR-II genotype. Bioinformatics analysis of the sequences of 40 years of Brazilian IBV variants was performed and the ratio of non-synonymous substitutions per non-synonymous site (dn) to synonymous substitutions per synonymous site (ds) dN/dS was calculated. It revealed a predominance of codons with non-synonymous substitutions in the first third of the S1 gene and a dN/dS ratio of 0.6757, indicating that this portion of the gene was under negative selection. Additionally prediction of N-glycosilation sites showed that most of the BR-I variants (from 2003 to early 2014) present an extra site at animoacid position 20, while the newest ones lack this feature.Together these results suggest that IBV Brazilian variants had probably suffered drastic mutations in some points between the years 1983 to 2003 and after achieving an antigenic structure effective enough for invasion and replication in their hosts, the selection processes became silent. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04032016-154921/ |
url |
http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04032016-154921/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
_version_ |
1815257700696588288 |