Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity

Detalhes bibliográficos
Ano de defesa: 2023
Autor(a) principal: Anjos, Victor Federico Leal dos
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: eng
Instituição de defesa: Biblioteca Digitais de Teses e Dissertações da USP
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: https://www.teses.usp.br/teses/disponiveis/11/11139/tde-05062023-152311/
Resumo: The whole plant corn silage (WPCS) is the most used roughage in the diet of beef and dairy cattle in Brazil. The use of technologies in the making of silage has been increasing in recent years in order to ensure a more efficient harvest and a good fermentation standard for the silos. However, in some regions of the country, the rainfall deficit generates water stress in plants, altering the chemical quality and profile of carbohydrates available for fermentation. On the other hand, the presence of rain at the time of harvesting can also cause delays. In many cases, harvesting maize plants with high dry matter leads to considerable fermentation losses in the silage. Rehydrating the plant according to some researchers and field technicians can improve the conservation of plants with high DM. The advantages and the way that corn grain rehydration should be done in order to take better use of the starch is well described in the literature. However, there are no studies evaluating how SMPI rehydration can improve the fermentative aspects of the silo environment. In this sense, the objective of this study was to evaluate the losses and fermentative profile of silages with DM above the recommended level and silages harvested with high DM and which received water addition in order to reduce the DM to the recommended standard. Five harvests were performed at 7-day intervals each, where in the first harvest the mass was ensiled without adding water (34.7%), in the other harvests the materials were ensiled with the respective DM for the harvest (40.7; 46.9, 49.5 and 54.2%) and ensiled with the addition of water (34.9; 35.5; 34.0; 33.7%) so that DM returned close to that found in the first harvest. The materials were ensiled in experimental silos (20 L buckets) containing 2 kg of sand at the bottom separated by a screen and cloth in order to measure the production of effluents (EL) and equipped with lids for complete sealing. The storage time was 90 days. The treatments were arranged in a factorial scheme with four replications (2*4+1) being 2 with and without water addition; 4 DM at the time of harvest and 1 the positive control treatment. The data were analyzed using the SAS MIXED procedure. Fermentative losses, pH, lactic acid, volatile fatty acids, ethanol, microorganisms, aerobic stability (A.E), density, mean particle distribution, water holding capacity (WHC) and bromatological were evaluated. The pH of the materials that received the addition of water were lower compared to the treatments without the addition of water and did not differ from the positive control. Lactic acid production and lactic acid fermenting bacteria (LAB) counts were also higher in rehydrated treatments. There was no difference between any of the treatments for clostridia. The higher the DM at the time of harvest, the greater the addition of water, which led to greater losses by effluents, the treatment with DAE 135 and 36.8% DM obtained the highest losses by effluents. Density was also altered during rehydration, plants with higher dry matter linearly decreased density, while rehydrated treatments maintained initial density, only the treatment with DAE 135 and 36.8% DM differed. The average rehydrated treatments also showed higher A.E., but no treatment showed good A.E. Those with high DM were losing WHC. In conclusion, the results suggest that adding water can be a good strategy to reduce losses and improve the fermentative pattern and maize plants harvested with high DM. However, not every plant has the capacity to retain additional water, materials with DM above 55% showed greater production of effluents, which generates greater losses and greater environmental risk.
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spelling Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturityMicrobiologia e perdas fermentativas de silagem de planta inteira reidratada colhida em maturidade avançadaColheita tardiaFermentative lossesHigh DMLate harvestMS elevadaPerdas fermentativasRehydrationReidrataçãoThe whole plant corn silage (WPCS) is the most used roughage in the diet of beef and dairy cattle in Brazil. The use of technologies in the making of silage has been increasing in recent years in order to ensure a more efficient harvest and a good fermentation standard for the silos. However, in some regions of the country, the rainfall deficit generates water stress in plants, altering the chemical quality and profile of carbohydrates available for fermentation. On the other hand, the presence of rain at the time of harvesting can also cause delays. In many cases, harvesting maize plants with high dry matter leads to considerable fermentation losses in the silage. Rehydrating the plant according to some researchers and field technicians can improve the conservation of plants with high DM. The advantages and the way that corn grain rehydration should be done in order to take better use of the starch is well described in the literature. However, there are no studies evaluating how SMPI rehydration can improve the fermentative aspects of the silo environment. In this sense, the objective of this study was to evaluate the losses and fermentative profile of silages with DM above the recommended level and silages harvested with high DM and which received water addition in order to reduce the DM to the recommended standard. Five harvests were performed at 7-day intervals each, where in the first harvest the mass was ensiled without adding water (34.7%), in the other harvests the materials were ensiled with the respective DM for the harvest (40.7; 46.9, 49.5 and 54.2%) and ensiled with the addition of water (34.9; 35.5; 34.0; 33.7%) so that DM returned close to that found in the first harvest. The materials were ensiled in experimental silos (20 L buckets) containing 2 kg of sand at the bottom separated by a screen and cloth in order to measure the production of effluents (EL) and equipped with lids for complete sealing. The storage time was 90 days. The treatments were arranged in a factorial scheme with four replications (2*4+1) being 2 with and without water addition; 4 DM at the time of harvest and 1 the positive control treatment. The data were analyzed using the SAS MIXED procedure. Fermentative losses, pH, lactic acid, volatile fatty acids, ethanol, microorganisms, aerobic stability (A.E), density, mean particle distribution, water holding capacity (WHC) and bromatological were evaluated. The pH of the materials that received the addition of water were lower compared to the treatments without the addition of water and did not differ from the positive control. Lactic acid production and lactic acid fermenting bacteria (LAB) counts were also higher in rehydrated treatments. There was no difference between any of the treatments for clostridia. The higher the DM at the time of harvest, the greater the addition of water, which led to greater losses by effluents, the treatment with DAE 135 and 36.8% DM obtained the highest losses by effluents. Density was also altered during rehydration, plants with higher dry matter linearly decreased density, while rehydrated treatments maintained initial density, only the treatment with DAE 135 and 36.8% DM differed. The average rehydrated treatments also showed higher A.E., but no treatment showed good A.E. Those with high DM were losing WHC. In conclusion, the results suggest that adding water can be a good strategy to reduce losses and improve the fermentative pattern and maize plants harvested with high DM. However, not every plant has the capacity to retain additional water, materials with DM above 55% showed greater production of effluents, which generates greater losses and greater environmental risk.A silagem de milho planta inteira (SMPI) é o volumoso mais utilizado na dieta de bovinos de corte e leite no Brasil. O uso de tecnologias na confecção de silagem vem aumentando nos últimos anos, a fim de garantir uma colheita mais eficiente e um bom padrão fermentativo para os silos. Entretanto, em algumas regiões do país o déficit de chuvas gera estresse hídrico nas plantas, alterando a qualidade química e perfil de carboidratos disponíveis para a fermentação. Em contrapartida a presença de chuva no momento da colheita também pode gerar atrasos. Em muitos, casos a colheita de plantas de milho com matéria seca elevada gera perdas fermentativas consideráveis da silagem. Reidratadar a planta, segundo alguns pesquisadores e técnicos de campo, pode apresentar melhorias na conservação de plantas com MS elevada. As vantagens e a forma que deve ser feita reidratação de grãos de milho objetivando maior aproveitamento de amido é bem descrita na literatura. Porém, não existem estudos avaliando como a reidratação de SMPI pode melhorar em quesitos fermentativos o ambiente do silo. Nesse sentido, o objetivo deste estudo foi avaliar as perdas e o perfil fermentativo de silagens com MS acima do recomendado e silagens colhidas com MS alta e que receberam adição de água com o intuito de reduzir a MS para o padrão recomendado. Foram realizadas 5 colheitas com intervalos de 7 dias cada uma, onde na primeira colheita a massa foi ensilada sem adição de água (37,5%), nas demais colheitas os materiais foram ensilados com a MS respectiva a colheita (40,7; 46,9, 49,5 and 54,2%) e ensilados com a adição de água (34,9; 35,5; 34,0; 33,7%) para que a MS retornasse próximo à encontrada na primeira colheita. Os materiais foram ensilados em silo experimentais (baldes de 20 L) contendo 2 kg de areia no fundo separada por tela e pano afim de mensurar a produção de efluentes (PE) e equipados com tampas para total vedação. O tempo de armazenamento foi de 90 dias. Os tratamentos foram arranjados em esquema fatorial com quatro repetições (2*4+1) sendo 2 com e sem adição de água; 4 MS no momento da colheita e 1 o tratamento controle positivo. Os dados foram analisados utilizando o procedimento MIXED do SAS. Foram avaliadas as perdas fermentativas, pH, ácido lático, ácidos graxos voláteis, etanol, microrganismos, estabilidade aeróbica (E.A), densidade, distribuição média de partículas, capacidade de retenção de água (CRA) e bromatológica. O ph dos materiais que receberam a adição de água foram menores em comparação aos tratamentos sem adição de água e não diferiram do controle positivo. A produção de ácidos lático e a contagem de bactérias fermentadoras de ácidos lático (BAL) também foram maiores nos tratamentos reidratados. Não houve diferença entre nenhum dos tratamentos para clostridios. Quanto mais elevada a MS no momento da colheita, maior foi a adição de água, o que levou a maiores perdas por efluentes, o tratamento com DAE 135 e 36,8% de MS obteve as maiores perdas por efluentes. A densidade também foi alterada na reidratação, as plantas com maior matéria seca foram diminuindo linearmente a densidade, enquanto os tratamentos reidratados mantiveram densidade inicial, apenas o tratamento com DAE 135 e 36,8% de MS se diferenciou. Os tratamentos reidratados na média também apresentaram maior E.A, mas nenhum tratamento apresentou boa E.A. Os matérias com MS elevada foram perdendo CRA. Em conclusão, os resultados sugerem que adicionar água pode ser uma boa estratégia para reduzir as perdas e melhorar o padrão fermentativos e plantas de milho colhidas com MS elevada. Entretanto nem toda planta possui a capacidade de reter a água adicional, materiais com MS acima de 55% apresentaram maior produção de efluentes, o que gera maiores perdas e maior risco ambiental.Biblioteca Digitais de Teses e Dissertações da USPNussio, Luiz GustavoAnjos, Victor Federico Leal dos2023-04-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/11/11139/tde-05062023-152311/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2023-06-06T13:07:40Zoai:teses.usp.br:tde-05062023-152311Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212023-06-06T13:07:40Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
Microbiologia e perdas fermentativas de silagem de planta inteira reidratada colhida em maturidade avançada
title Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
spellingShingle Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
Anjos, Victor Federico Leal dos
Colheita tardia
Fermentative losses
High DM
Late harvest
MS elevada
Perdas fermentativas
Rehydration
Reidratação
title_short Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
title_full Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
title_fullStr Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
title_full_unstemmed Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
title_sort Microbiology and fermentative losses of rehydrated whole plant silage harvested at high maturity
author Anjos, Victor Federico Leal dos
author_facet Anjos, Victor Federico Leal dos
author_role author
dc.contributor.none.fl_str_mv Nussio, Luiz Gustavo
dc.contributor.author.fl_str_mv Anjos, Victor Federico Leal dos
dc.subject.por.fl_str_mv Colheita tardia
Fermentative losses
High DM
Late harvest
MS elevada
Perdas fermentativas
Rehydration
Reidratação
topic Colheita tardia
Fermentative losses
High DM
Late harvest
MS elevada
Perdas fermentativas
Rehydration
Reidratação
description The whole plant corn silage (WPCS) is the most used roughage in the diet of beef and dairy cattle in Brazil. The use of technologies in the making of silage has been increasing in recent years in order to ensure a more efficient harvest and a good fermentation standard for the silos. However, in some regions of the country, the rainfall deficit generates water stress in plants, altering the chemical quality and profile of carbohydrates available for fermentation. On the other hand, the presence of rain at the time of harvesting can also cause delays. In many cases, harvesting maize plants with high dry matter leads to considerable fermentation losses in the silage. Rehydrating the plant according to some researchers and field technicians can improve the conservation of plants with high DM. The advantages and the way that corn grain rehydration should be done in order to take better use of the starch is well described in the literature. However, there are no studies evaluating how SMPI rehydration can improve the fermentative aspects of the silo environment. In this sense, the objective of this study was to evaluate the losses and fermentative profile of silages with DM above the recommended level and silages harvested with high DM and which received water addition in order to reduce the DM to the recommended standard. Five harvests were performed at 7-day intervals each, where in the first harvest the mass was ensiled without adding water (34.7%), in the other harvests the materials were ensiled with the respective DM for the harvest (40.7; 46.9, 49.5 and 54.2%) and ensiled with the addition of water (34.9; 35.5; 34.0; 33.7%) so that DM returned close to that found in the first harvest. The materials were ensiled in experimental silos (20 L buckets) containing 2 kg of sand at the bottom separated by a screen and cloth in order to measure the production of effluents (EL) and equipped with lids for complete sealing. The storage time was 90 days. The treatments were arranged in a factorial scheme with four replications (2*4+1) being 2 with and without water addition; 4 DM at the time of harvest and 1 the positive control treatment. The data were analyzed using the SAS MIXED procedure. Fermentative losses, pH, lactic acid, volatile fatty acids, ethanol, microorganisms, aerobic stability (A.E), density, mean particle distribution, water holding capacity (WHC) and bromatological were evaluated. The pH of the materials that received the addition of water were lower compared to the treatments without the addition of water and did not differ from the positive control. Lactic acid production and lactic acid fermenting bacteria (LAB) counts were also higher in rehydrated treatments. There was no difference between any of the treatments for clostridia. The higher the DM at the time of harvest, the greater the addition of water, which led to greater losses by effluents, the treatment with DAE 135 and 36.8% DM obtained the highest losses by effluents. Density was also altered during rehydration, plants with higher dry matter linearly decreased density, while rehydrated treatments maintained initial density, only the treatment with DAE 135 and 36.8% DM differed. The average rehydrated treatments also showed higher A.E., but no treatment showed good A.E. Those with high DM were losing WHC. In conclusion, the results suggest that adding water can be a good strategy to reduce losses and improve the fermentative pattern and maize plants harvested with high DM. However, not every plant has the capacity to retain additional water, materials with DM above 55% showed greater production of effluents, which generates greater losses and greater environmental risk.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-03
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dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
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