Desenvolvimento de marcadores do tipo SSRs para genes candidatos relacionados com a qualidade da carne em Bos taurus

• Ano de defesa: 2021
• Autor(a) principal: Ribeiro, Carmen Catarina Brostolin
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Tecnológica Federal do Paraná; Dois Vizinhos; Brasil; Programa de Pós-Graduação em Zootecnia; UTFPR
• Palavras-chave em Português: Bovinos de corte; Marcadores genéticos; Genética animal; Beef cattle; Genetic markers; Animal genetics; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::PRODUCAO ANIMAL; Produção Animal
• Link de acesso: http://repositorio.utfpr.edu.br/jspui/handle/1/28052

Beef cattle farming uses molecular markers to verify the location of genes for meat production and quality, reproductive aspects and disease resistance. With this purpose, the objective was to identify and develop microsatellite markers (SSRs) for genes related to production and meat quality of the Marchangus compound. With the Panther 16.0 program, function and pathway enrichment was performed through the genomic sequence of Bos taurus for the genes related to meat quality (DGAT1, CAPN1, CAST, TG, PRLR, CAPN2, CAPN3) available in the National Center database For Biotechnology Information (NCBI) and, with the String tool, a protein-protein interaction network was built. The identification and location of the SSRs and the design of the initiators were performed using Krait software, based on the download of sequences in the FASTA format. The validation of the primers was performed in silico using the SMS program - PCR Primer Stats, to confirm the absence of hairpins and dimers, the BLASTn was used to verify the alignment of the primers to the gene sequences, and the SMS PCR Products program, who are looking for places with perfect annealing. Laboratory validation was performed using genetic material from the blood of 25 cattle of the Marchangus Compound. The result of the ontological analysis of candidate genes for meat quality demonstrated that they are associated with complex biological activities, including hormonal regulation, lipid activity, and the process of protein degradation by enzymes. The protein-protein interaction network confirms that there is an interconnection between the genes studied, but the main association occurs in the calpain-calpastatin enzyme complex. The number of microsatellite markers (SSRs) developed for each gene was: DAGT1 (2), CAPN1 (4),CAST (47), TG (65), PRLR (54), CAPN2 (14) and CAPN3 (8). Based on the obtained SSRs, the designer of the primers was performed. After virtual validation, the results of the primers were as follows: CAPN1 (1), CAST (4) and PRLR (1). The other initiators did not demonstrate adequate standards for performing genotyping. Therefore, it can be concluded from the tests performed that from the bioinformatics analysis it is possible to develop efficient primers, both with virtual PCR and with conventional PCR. The study of polymorphisms by molecular techniques for candidate genes will allow the improvement of the trait of zootechnical interest, as well as the use of these markers in genetic mapping and assisted selection programs.