Agentes de controle para o manejo de molicutes na cultura do milho

Detalhes bibliográficos
Ano de defesa: 2025
Autor(a) principal: Carini, Bruno
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Tecnológica Federal do Paraná
Pato Branco
Brasil
Programa de Pós-Graduação em Agronomia
UTFPR
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.utfpr.edu.br/jspui/handle/1/38567
Resumo: Maize (Zea mays) is one of Brazil's main agricultural crops, widely used as a source of human and animal food. However, the crop's productivity has been impacted by the infestation of the corn leafhopper Dalbulus maidis (Hemiptera: Cicadellidae), a vector of pathogens responsible for the diseases that make up the Corn Blight Complex. This damage highlights the need for effective management strategies to control the pest. The aim of this study was to evaluate, under field conditions, the effectiveness of products based on the entomopathogenic fungi Beauveria bassiana and Cordyceps javanica as components of the integrated management of D. maidis. The experiments were conducted in a network, at cooperative experimental stations located in the municipalities of Campo Mourão, Cafelândia, Assaí and Floresta, in the state of Paraná, and in Sete Lagoas, Minas Gerais, during the second harvest of 2024. The treatments tested consisted of six combinations of chemical (methomyl and acephate) and biological (Beauveria bassiana and Cordyceps javanica) products, applied from the V3 stage of the AS 1844 hybrid In all the treatments, acephate was applied at the V1 and V2 stages to control the green-bellied stink bug (Diceraeus melacanthus). Control was established using plants treated exclusively with products aimed at the bug. The severity of the blights was assessed using rating scales, and corn yield was determined based on the ratio between the quantity of grains harvested and the area cultivated (Kg/ha). In addition, the molecular detection of the mollicutes “Candidatus Phytoplasma asteris” and Spiroplasma kunkelii was carried out via specific PCR in leaf and stalk samples. The joint analysis of the experiments indicated that the treatment of the plants with methomyl combined with Beauveria bassiana (T3) was the most efficient, as it showed the lowest severity score for blight (2.40), which corresponds to less than 25% of leaves showing symptoms of the disease. However, treatment T3 recorded the highest rate of molecular detection of the mollicutes, with 80% of the samples showing DNA from the pathogens. Treatment T7 (without application) showed the highest yield, reaching 8147.5 kg/ha. The results indicate that the use of entomopathogenic fungi can contribute to the control of D. maidis. However, there were no statistically significant differences between the chemical and biological treatments in terms of productivity.
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spelling Agentes de controle para o manejo de molicutes na cultura do milhoControl agents for managing molicutes in corn cropsPragas - Controle biológicoMilho - Doenças e pragasPragas agrícolas - Controle biológicoProdutividade agrícolaPests - Biological controlCorn - Diseases and pestsAgricultural pests - Biological controlAgricultural productivityCNPQ::CIENCIAS AGRARIAS::AGRONOMIAAgronomiaMaize (Zea mays) is one of Brazil's main agricultural crops, widely used as a source of human and animal food. However, the crop's productivity has been impacted by the infestation of the corn leafhopper Dalbulus maidis (Hemiptera: Cicadellidae), a vector of pathogens responsible for the diseases that make up the Corn Blight Complex. This damage highlights the need for effective management strategies to control the pest. The aim of this study was to evaluate, under field conditions, the effectiveness of products based on the entomopathogenic fungi Beauveria bassiana and Cordyceps javanica as components of the integrated management of D. maidis. The experiments were conducted in a network, at cooperative experimental stations located in the municipalities of Campo Mourão, Cafelândia, Assaí and Floresta, in the state of Paraná, and in Sete Lagoas, Minas Gerais, during the second harvest of 2024. The treatments tested consisted of six combinations of chemical (methomyl and acephate) and biological (Beauveria bassiana and Cordyceps javanica) products, applied from the V3 stage of the AS 1844 hybrid In all the treatments, acephate was applied at the V1 and V2 stages to control the green-bellied stink bug (Diceraeus melacanthus). Control was established using plants treated exclusively with products aimed at the bug. The severity of the blights was assessed using rating scales, and corn yield was determined based on the ratio between the quantity of grains harvested and the area cultivated (Kg/ha). In addition, the molecular detection of the mollicutes “Candidatus Phytoplasma asteris” and Spiroplasma kunkelii was carried out via specific PCR in leaf and stalk samples. The joint analysis of the experiments indicated that the treatment of the plants with methomyl combined with Beauveria bassiana (T3) was the most efficient, as it showed the lowest severity score for blight (2.40), which corresponds to less than 25% of leaves showing symptoms of the disease. However, treatment T3 recorded the highest rate of molecular detection of the mollicutes, with 80% of the samples showing DNA from the pathogens. Treatment T7 (without application) showed the highest yield, reaching 8147.5 kg/ha. The results indicate that the use of entomopathogenic fungi can contribute to the control of D. maidis. However, there were no statistically significant differences between the chemical and biological treatments in terms of productivity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do ParanáSecretaria da Ciência, Tecnologia e Ensino Superior (SETI)O milho (Zea mays) é um dos principais cultivos agrícolas do Brasil, amplamente utilizado como fonte de alimentação humana e animal. No entanto, a produtividade da cultura tem sido impactada pela infestação da cigarrinha-do-milho Dalbulus maidis (Hemiptera: Cicadellidae), vetor de patógenos responsáveis pelas doenças que integram o Complexo de enfezamento do Milho. Esses prejuízos evidenciam a necessidade de estratégias eficazes de manejo para o controle da praga. Este estudo teve como objetivo avaliar, em condições de campo, a eficácia de produtos à base dos fungos entomopatogênicos Beauveria bassiana e Cordyceps javanica como componentes do manejo integrado de D. maidis. Os experimentos foram conduzidos em rede, em estações experimentais de cooperativas situadas nos municípios de Campo Mourão, Cafelândia, Assaí e Floresta, no estado do Paraná, e em Sete Lagoas, Minas Gerais, durante a segunda safra de 2024. Os tratamentos testados consistiram em seis combinações de produtos químicos (metomil e acefato) e biológicos (Beauveria bassiana e Cordyceps javanica), aplicados a partir do estádio V3 do hibrido AS 1844 Em todos os tratamentos, realizaram-se aplicações de acefato nos estádios V1 e V2 para o controle do percevejo barriga-verde (Diceraeus melacanthus). O controle foi estabelecido por meio de plantas tratadas exclusivamente com produtos direcionados ao percevejo. A severidade dos enfezamentos foi avaliada por meio de escalas de notas, e a produtividade do milho foi determinada com base na relação entre a quantidade de grãos colhidos e a área cultivada (Kg/ha). Além disso, realizou-se a detecção molecular dos molicutes “Candidatus Phytoplasma asteris” e Spiroplasma kunkelii via PCR específico em amostras de folha e colmo. A análise conjunta dos experimentos indicou que o tratamento das plantas com metomil associado a Beauveria bassiana (T3) foi o mais eficiente, pois apresentou a menor nota severidade para enfezamento (2,40), o que corresponde a menos de 25% de folhas com sintomas da doença. No entanto, o tratamento T3 registrou a maior taxa de detecção molecular dos molicutes, com 80% das amostras apresentando DNA dos patógenos.O tratamento T7 (sem aplicação) demonstrou a maior produtividade, atingindo 8147,5 kg/ha. Os resultados indicam que o uso de fungos entomopatogênicos pode contribuir para o controle de D. maidis. No entanto, não foram observadas diferenças estatisticamente significativas entre os tratamentos químicos e biológicos no que diz respeito à produtividade.Universidade Tecnológica Federal do ParanáPato BrancoBrasilPrograma de Pós-Graduação em AgronomiaUTFPRPotrich, Michelehttps://orcid.org/0000-0002-4861-5536https://lattes.cnpq.br/6017285848848713Andrade, Gilberto Santoshttps://orcid.org/0000-0001-7234-2980https://lattes.cnpq.br/3683885119599212Silva, Michele Regina Lopes dahttps://orcid.org/0000-0003-4016-1813http://lattes.cnpq.br/2224006023152285Ventura, Mauricio Ursihttp://lattes.cnpq.br/4519731713713970Potrich, Michelehttps://orcid.org/0000-0002-4861-5536https://lattes.cnpq.br/6017285848848713Carini, Bruno2025-10-06T13:49:51Z2025-10-06T13:49:51Z2025-03-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfCARINI, Bruno. Agentes de controle para o manejo de molicutes na cultura do milho. 2025. Dissertação (Mestrado em Agronomia) - Universidade Tecnológica Federal do Paraná, Pato Branco, 2025.http://repositorio.utfpr.edu.br/jspui/handle/1/38567porhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT))instname:Universidade Tecnológica Federal do Paraná (UTFPR)instacron:UTFPR2025-10-07T06:15:09Zoai:repositorio.utfpr.edu.br:1/38567Repositório InstitucionalPUBhttp://repositorio.utfpr.edu.br:8080/oai/requestriut@utfpr.edu.br || sibi@utfpr.edu.bropendoar:2025-10-07T06:15:09Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT)) - Universidade Tecnológica Federal do Paraná (UTFPR)false
dc.title.none.fl_str_mv Agentes de controle para o manejo de molicutes na cultura do milho
Control agents for managing molicutes in corn crops
title Agentes de controle para o manejo de molicutes na cultura do milho
spellingShingle Agentes de controle para o manejo de molicutes na cultura do milho
Carini, Bruno
Pragas - Controle biológico
Milho - Doenças e pragas
Pragas agrícolas - Controle biológico
Produtividade agrícola
Pests - Biological control
Corn - Diseases and pests
Agricultural pests - Biological control
Agricultural productivity
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
Agronomia
title_short Agentes de controle para o manejo de molicutes na cultura do milho
title_full Agentes de controle para o manejo de molicutes na cultura do milho
title_fullStr Agentes de controle para o manejo de molicutes na cultura do milho
title_full_unstemmed Agentes de controle para o manejo de molicutes na cultura do milho
title_sort Agentes de controle para o manejo de molicutes na cultura do milho
author Carini, Bruno
author_facet Carini, Bruno
author_role author
dc.contributor.none.fl_str_mv Potrich, Michele
https://orcid.org/0000-0002-4861-5536
https://lattes.cnpq.br/6017285848848713
Andrade, Gilberto Santos
https://orcid.org/0000-0001-7234-2980
https://lattes.cnpq.br/3683885119599212
Silva, Michele Regina Lopes da
https://orcid.org/0000-0003-4016-1813
http://lattes.cnpq.br/2224006023152285
Ventura, Mauricio Ursi
http://lattes.cnpq.br/4519731713713970
Potrich, Michele
https://orcid.org/0000-0002-4861-5536
https://lattes.cnpq.br/6017285848848713
dc.contributor.author.fl_str_mv Carini, Bruno
dc.subject.por.fl_str_mv Pragas - Controle biológico
Milho - Doenças e pragas
Pragas agrícolas - Controle biológico
Produtividade agrícola
Pests - Biological control
Corn - Diseases and pests
Agricultural pests - Biological control
Agricultural productivity
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
Agronomia
topic Pragas - Controle biológico
Milho - Doenças e pragas
Pragas agrícolas - Controle biológico
Produtividade agrícola
Pests - Biological control
Corn - Diseases and pests
Agricultural pests - Biological control
Agricultural productivity
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
Agronomia
description Maize (Zea mays) is one of Brazil's main agricultural crops, widely used as a source of human and animal food. However, the crop's productivity has been impacted by the infestation of the corn leafhopper Dalbulus maidis (Hemiptera: Cicadellidae), a vector of pathogens responsible for the diseases that make up the Corn Blight Complex. This damage highlights the need for effective management strategies to control the pest. The aim of this study was to evaluate, under field conditions, the effectiveness of products based on the entomopathogenic fungi Beauveria bassiana and Cordyceps javanica as components of the integrated management of D. maidis. The experiments were conducted in a network, at cooperative experimental stations located in the municipalities of Campo Mourão, Cafelândia, Assaí and Floresta, in the state of Paraná, and in Sete Lagoas, Minas Gerais, during the second harvest of 2024. The treatments tested consisted of six combinations of chemical (methomyl and acephate) and biological (Beauveria bassiana and Cordyceps javanica) products, applied from the V3 stage of the AS 1844 hybrid In all the treatments, acephate was applied at the V1 and V2 stages to control the green-bellied stink bug (Diceraeus melacanthus). Control was established using plants treated exclusively with products aimed at the bug. The severity of the blights was assessed using rating scales, and corn yield was determined based on the ratio between the quantity of grains harvested and the area cultivated (Kg/ha). In addition, the molecular detection of the mollicutes “Candidatus Phytoplasma asteris” and Spiroplasma kunkelii was carried out via specific PCR in leaf and stalk samples. The joint analysis of the experiments indicated that the treatment of the plants with methomyl combined with Beauveria bassiana (T3) was the most efficient, as it showed the lowest severity score for blight (2.40), which corresponds to less than 25% of leaves showing symptoms of the disease. However, treatment T3 recorded the highest rate of molecular detection of the mollicutes, with 80% of the samples showing DNA from the pathogens. Treatment T7 (without application) showed the highest yield, reaching 8147.5 kg/ha. The results indicate that the use of entomopathogenic fungi can contribute to the control of D. maidis. However, there were no statistically significant differences between the chemical and biological treatments in terms of productivity.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-06T13:49:51Z
2025-10-06T13:49:51Z
2025-03-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv CARINI, Bruno. Agentes de controle para o manejo de molicutes na cultura do milho. 2025. Dissertação (Mestrado em Agronomia) - Universidade Tecnológica Federal do Paraná, Pato Branco, 2025.
http://repositorio.utfpr.edu.br/jspui/handle/1/38567
identifier_str_mv CARINI, Bruno. Agentes de controle para o manejo de molicutes na cultura do milho. 2025. Dissertação (Mestrado em Agronomia) - Universidade Tecnológica Federal do Paraná, Pato Branco, 2025.
url http://repositorio.utfpr.edu.br/jspui/handle/1/38567
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dc.publisher.none.fl_str_mv Universidade Tecnológica Federal do Paraná
Pato Branco
Brasil
Programa de Pós-Graduação em Agronomia
UTFPR
publisher.none.fl_str_mv Universidade Tecnológica Federal do Paraná
Pato Branco
Brasil
Programa de Pós-Graduação em Agronomia
UTFPR
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reponame_str Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT))
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