Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae
Ano de defesa: | 2010 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Mestrado em Ciências Farmacêuticas
|
Departamento: |
Ciências da Saúde - Farmácia
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tde/2095 |
Resumo: | The Pterodon emarginatus Vogel, known as sucupira branca (Fabaceae) has been shown to possess larvicidal activity against (Aedes aegypti), antiparasitic effect (Leishmania amazonensis), fungicide, bactericide, antioxidant, antiinflammatory, antinociceptive, and antiproliferative activity among several different human cancer cell lines that may be related to presence of diterpenes, in particular, vouacapanes derivatives. This work aimed to define and characterize the powder, the crude and standardized dry extract of the fruit of sucupira . After crushing the fruits, showed 1.2% volatile. The chromatographic (TLC) indicated the presence of chemical markers vouacapanes (6α,7β-dihydroxyvouacapan-17β-oic acid - Rf = 0.16; 6α,7β-dihydroxyvouacapan-17β-oate methyl ester - Rf = 0.44; 6α-hydroxyvouacapan-7β-17β-lactone - Rf = 0.71) and lupeol (Rf = 0.78). In vibration bands in the infrared region coinciding with the five main bands of vouacapanes (-OH, C-H , C=O, C=C, C-O). The concentration of total terpenes of 9.3%. Then to obtain the crude extract was held percolation in 95% ethanol PA. The crude extract showed pH 5.1, relative density of 0.87, viscosity of 25.43 mPas, solid 15.2%, total terpenes of 17.1% and the same chromatographic profile and in the infrared spectrum obtained for the powder plant. Another method used for investigation of terpenes was the gas chromatography / mass spectrometry in the hexane extract. We identified the presence of vouacapanes and the lupeol. The drying process of the crude extract was used nebulization (spray drying) using different adjuvants drying, flow of 0.19 L / h, air flow 35 L / min, inlet temperature 110°C, outlet temperature 97°C, double fluid nozzle spray type 1.2 mm. The best results were obtained with the colloidal silicon dioxide (Aerosil®) to 20%, giving an appearance of fine powder. Thermal analysis performed for the marker 6α,7β-dihydroxyvouacapan-17β-oate methyl ester showed that the drying parameters were safe by preventing the thermal degradation of diterpenes. The efficiency of the drying process was 16.2%, degradation of 10.9% and the concentration of terpenes total in dry extract was 13.9%. The chromatographic profile was maintained and the volatile content was 3.5%. The photomicrographs showed irregular and spherical particles, rough surface. The analytical method by spectrophotometry in the visible region for the quantification of total terpenes in the dust, crude extract and dry extract was validated presenting selective, linear, precise, accurate and robust. In the test of antinociceptive activity (capsaicin), the pre-treatment with dry extract of sucupira (SDE 1000 mg / kg + 20% DMSO) reduced the time reactivity in 50.9% compared to the vehicle. The results may suggest that the technological processes employed to transform the fruits of sucupira in standardized dry extract was adequate to maintain quality chemistry and antinociceptive activity described for the fruits. This work represents the first description of the taking of standardized dry extract and identification of lupeol in fruits of sucupira . |
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BARA, Maria Teresa Freitashttp://lattes.cnpq.br/3914164125498267CONCEIÇÃO, Edemilson Cardoso dahttp://lattes.cnpq.br/7193007113950510OLIVEIRA, P. C.OLIVEIRA, Patrícya Caixeta de2014-07-29T16:11:47Z2011-02-252010-08-31OLIVEIRA, Patrícya Caixeta de. Collection and Characterization of Standardized Dry Extract of the Fruit of Sucupira Pterodon emarginatus, Fabaceae. 2010. 24 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2010.http://repositorio.bc.ufg.br/tede/handle/tde/2095The Pterodon emarginatus Vogel, known as sucupira branca (Fabaceae) has been shown to possess larvicidal activity against (Aedes aegypti), antiparasitic effect (Leishmania amazonensis), fungicide, bactericide, antioxidant, antiinflammatory, antinociceptive, and antiproliferative activity among several different human cancer cell lines that may be related to presence of diterpenes, in particular, vouacapanes derivatives. This work aimed to define and characterize the powder, the crude and standardized dry extract of the fruit of sucupira . After crushing the fruits, showed 1.2% volatile. The chromatographic (TLC) indicated the presence of chemical markers vouacapanes (6α,7β-dihydroxyvouacapan-17β-oic acid - Rf = 0.16; 6α,7β-dihydroxyvouacapan-17β-oate methyl ester - Rf = 0.44; 6α-hydroxyvouacapan-7β-17β-lactone - Rf = 0.71) and lupeol (Rf = 0.78). In vibration bands in the infrared region coinciding with the five main bands of vouacapanes (-OH, C-H , C=O, C=C, C-O). The concentration of total terpenes of 9.3%. Then to obtain the crude extract was held percolation in 95% ethanol PA. The crude extract showed pH 5.1, relative density of 0.87, viscosity of 25.43 mPas, solid 15.2%, total terpenes of 17.1% and the same chromatographic profile and in the infrared spectrum obtained for the powder plant. Another method used for investigation of terpenes was the gas chromatography / mass spectrometry in the hexane extract. We identified the presence of vouacapanes and the lupeol. The drying process of the crude extract was used nebulization (spray drying) using different adjuvants drying, flow of 0.19 L / h, air flow 35 L / min, inlet temperature 110°C, outlet temperature 97°C, double fluid nozzle spray type 1.2 mm. The best results were obtained with the colloidal silicon dioxide (Aerosil®) to 20%, giving an appearance of fine powder. Thermal analysis performed for the marker 6α,7β-dihydroxyvouacapan-17β-oate methyl ester showed that the drying parameters were safe by preventing the thermal degradation of diterpenes. The efficiency of the drying process was 16.2%, degradation of 10.9% and the concentration of terpenes total in dry extract was 13.9%. The chromatographic profile was maintained and the volatile content was 3.5%. The photomicrographs showed irregular and spherical particles, rough surface. The analytical method by spectrophotometry in the visible region for the quantification of total terpenes in the dust, crude extract and dry extract was validated presenting selective, linear, precise, accurate and robust. In the test of antinociceptive activity (capsaicin), the pre-treatment with dry extract of sucupira (SDE 1000 mg / kg + 20% DMSO) reduced the time reactivity in 50.9% compared to the vehicle. The results may suggest that the technological processes employed to transform the fruits of sucupira in standardized dry extract was adequate to maintain quality chemistry and antinociceptive activity described for the fruits. This work represents the first description of the taking of standardized dry extract and identification of lupeol in fruits of sucupira .A Pterodon emarginatus Vogel, conhecida popularmente como Sucupira Branca, pertence à família Fabaceae. Estudos científicos comprovaram atividades larvicida (Aedes aegypti), antiparasitária (Leishmania amazonensis), fungicida, bactericida, antioxidante, antiinflamatória, antinociceptiva e anti-tumoral nos frutos dessa espécie, que podem estar relacionadas com a presença de diterpenos, de modo particular, os derivados vouacapânicos. Esse trabalho teve como objetivo obter e caracterizar o pó, o extrato bruto e extrato seco padronizado dos frutos da sucupira. O material botânico foi moído, obtendo-se um pó grosso, apresentando 1,2% de voláteis. O perfil cromatográfico (CCD) indicou a presença dos marcadores vouacapânicos (ácido 6α, 7β, di-hidroxivouacapan-17-β-óico - Rf = 0,16; 6α, 7β, di-hidroxivouacapan-17-β-oato de metila Rf = 0,44; 6α, hidroxivouacapan-7β, 17β-lactona Rf = 0,71) e lupeol (Rf = 0,78). Foram observadas bandas de vibração na região do infravermelho coincidentes com as cinco bandas principais dos vouacapanos (-OH, C-H , C=O, C=C, C-O). O teor de terpenos totais foi de 9,3%. Procedeu-se a obtenção do extrato bruto por percolação em etanol a 95% P.A. Este apresentou pH 5,1, densidade relativa de 0,87, viscosidade de 25,43 mPas, teor de sólidos de 15,2%, teor de terpenos totais de 17,1%, o mesmo perfil cromatográfico e espectro na região do infravermelho obtido para o pó do fruto. Outro método utilizado para investigação de terpenos foi a cromatografia gasosa acoplada à espectrometria de massas realizada no extrato hexânico. Identificou-se a presença de voaucapanos e do lupeol. Em seguida, realizou-se o processo de secagem do extrato bruto por nebulização (spray drying), utilizando diferentes adjuvantes de secagem, fluxo de 0,19 L/h, vazão do ar 35 L/min, temperatura de entrada 110°C, temperatura de saída 97°C, bico aspersor tipo duplo fluido 1,2 mm. Os melhores resultados foram obtidos com o dióxido de silício coloidal (Aerosil®) a 20%, proporcionando um aspecto de pó fino. A análise térmica realizada para o marcador 6, 7-dihidroxivouacapan-17-oato de metila demonstrou que os parâmetros de secagem foram seguros prevenindo a degradação térmica dos diterpenos. O rendimento do processo de secagem foi de 16,2%, a porcentagem de degradação de 10,9% e o teor de terpenos totais no extrato seco foi de 13,9%. O perfil cromatográfico foi mantido e o teor de voláteis foi de 3,5%. As fotomicrografias obtidas para o extrato seco mostraram partículas de formato irregular e esférico e superfície rugosa. O método analítico desenvolvido por espectrofotometria na região do visível para a quantificação dos terpenos totais no pó, extrato bruto e extrato seco foi validado apresentando-se seletivo, linear, preciso, exato e robusto. No teste de atividade antinociceptiva (capsaicina), o pré-tratamento com extrato seco de sucupira (ESS 1000 mg/kg + DMSO 20%) reduziu o tempo de reatividade em 50,9% em relação ao veículo. Os resultados obtidos permitem sugerir que o processamento tecnológico empregado para a transformação dos frutos da sucupira em extrato seco padronizado foi adequado, mantendo a qualidade química e a atividade antinociceptiva descritas. Esse trabalho representa a primeira descrição da obtenção do extrato seco padronizado e da identificação do lupeol nos frutos da sucupira.Made available in DSpace on 2014-07-29T16:11:47Z (GMT). No. of bitstreams: 1 dissertacao patricya c de oliveira.pdf: 350832 bytes, checksum: c35e2d06d9a2bd1b51c00c43002bf133 (MD5) Previous issue date: 2010-08-31application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/4530/dissertacao%20patricya%20c%20de%20oliveira.pdf.jpgporUniversidade Federal de GoiásMestrado em Ciências FarmacêuticasUFGBRCiências da Saúde - Farmáciaplanta medicinalfitoterápicoprocessamento tecnológicospray dryingcontrole de qualidadeantinociceptivomedicinal plantphytotherapictechnological processesspray dryingquality controlantinociceptiveCNPQ::CIENCIAS DA SAUDE::FARMACIAObtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, FabaceaeCollection and Characterization of Standardized Dry Extract of the Fruit of Sucupira Pterodon emarginatus, Fabaceaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALdissertacao patricya c de oliveira.pdfapplication/pdf350832http://repositorio.bc.ufg.br/tede/bitstreams/149e4582-2147-4f0a-ad5a-d7aeda99fe9a/downloadc35e2d06d9a2bd1b51c00c43002bf133MD51THUMBNAILdissertacao patricya c de oliveira.pdf.jpgdissertacao patricya c de oliveira.pdf.jpgGenerated Thumbnailimage/jpeg1943http://repositorio.bc.ufg.br/tede/bitstreams/851016d6-9fe5-41e2-b822-8e06f0516b66/downloadcc73c4c239a4c332d642ba1e7c7a9fb2MD52tde/20952014-07-30 03:14:44.312open.accessoai:repositorio.bc.ufg.br:tde/2095http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-07-30T06:14:44Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)false |
dc.title.por.fl_str_mv |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae |
dc.title.alternative.eng.fl_str_mv |
Collection and Characterization of Standardized Dry Extract of the Fruit of Sucupira Pterodon emarginatus, Fabaceae |
title |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae |
spellingShingle |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae OLIVEIRA, Patrícya Caixeta de planta medicinal fitoterápico processamento tecnológico spray drying controle de qualidade antinociceptivo medicinal plant phytotherapic technological processes spray drying quality control antinociceptive CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae |
title_full |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae |
title_fullStr |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae |
title_full_unstemmed |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae |
title_sort |
Obtenção e Caracterização do Extrato Seco Padronizado dos Frutos da Sucupira Pterodon emarginatus Vogel, Fabaceae |
author |
OLIVEIRA, Patrícya Caixeta de |
author_facet |
OLIVEIRA, Patrícya Caixeta de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
BARA, Maria Teresa Freitas |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3914164125498267 |
dc.contributor.advisor-co1.fl_str_mv |
CONCEIÇÃO, Edemilson Cardoso da |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7193007113950510 |
dc.contributor.authorLattes.fl_str_mv |
OLIVEIRA, P. C. |
dc.contributor.author.fl_str_mv |
OLIVEIRA, Patrícya Caixeta de |
contributor_str_mv |
BARA, Maria Teresa Freitas CONCEIÇÃO, Edemilson Cardoso da |
dc.subject.por.fl_str_mv |
planta medicinal fitoterápico processamento tecnológico spray drying controle de qualidade antinociceptivo |
topic |
planta medicinal fitoterápico processamento tecnológico spray drying controle de qualidade antinociceptivo medicinal plant phytotherapic technological processes spray drying quality control antinociceptive CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
medicinal plant phytotherapic technological processes spray drying quality control antinociceptive |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
The Pterodon emarginatus Vogel, known as sucupira branca (Fabaceae) has been shown to possess larvicidal activity against (Aedes aegypti), antiparasitic effect (Leishmania amazonensis), fungicide, bactericide, antioxidant, antiinflammatory, antinociceptive, and antiproliferative activity among several different human cancer cell lines that may be related to presence of diterpenes, in particular, vouacapanes derivatives. This work aimed to define and characterize the powder, the crude and standardized dry extract of the fruit of sucupira . After crushing the fruits, showed 1.2% volatile. The chromatographic (TLC) indicated the presence of chemical markers vouacapanes (6α,7β-dihydroxyvouacapan-17β-oic acid - Rf = 0.16; 6α,7β-dihydroxyvouacapan-17β-oate methyl ester - Rf = 0.44; 6α-hydroxyvouacapan-7β-17β-lactone - Rf = 0.71) and lupeol (Rf = 0.78). In vibration bands in the infrared region coinciding with the five main bands of vouacapanes (-OH, C-H , C=O, C=C, C-O). The concentration of total terpenes of 9.3%. Then to obtain the crude extract was held percolation in 95% ethanol PA. The crude extract showed pH 5.1, relative density of 0.87, viscosity of 25.43 mPas, solid 15.2%, total terpenes of 17.1% and the same chromatographic profile and in the infrared spectrum obtained for the powder plant. Another method used for investigation of terpenes was the gas chromatography / mass spectrometry in the hexane extract. We identified the presence of vouacapanes and the lupeol. The drying process of the crude extract was used nebulization (spray drying) using different adjuvants drying, flow of 0.19 L / h, air flow 35 L / min, inlet temperature 110°C, outlet temperature 97°C, double fluid nozzle spray type 1.2 mm. The best results were obtained with the colloidal silicon dioxide (Aerosil®) to 20%, giving an appearance of fine powder. Thermal analysis performed for the marker 6α,7β-dihydroxyvouacapan-17β-oate methyl ester showed that the drying parameters were safe by preventing the thermal degradation of diterpenes. The efficiency of the drying process was 16.2%, degradation of 10.9% and the concentration of terpenes total in dry extract was 13.9%. The chromatographic profile was maintained and the volatile content was 3.5%. The photomicrographs showed irregular and spherical particles, rough surface. The analytical method by spectrophotometry in the visible region for the quantification of total terpenes in the dust, crude extract and dry extract was validated presenting selective, linear, precise, accurate and robust. In the test of antinociceptive activity (capsaicin), the pre-treatment with dry extract of sucupira (SDE 1000 mg / kg + 20% DMSO) reduced the time reactivity in 50.9% compared to the vehicle. The results may suggest that the technological processes employed to transform the fruits of sucupira in standardized dry extract was adequate to maintain quality chemistry and antinociceptive activity described for the fruits. This work represents the first description of the taking of standardized dry extract and identification of lupeol in fruits of sucupira . |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-08-31 |
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2011-02-25 |
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2014-07-29T16:11:47Z |
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OLIVEIRA, Patrícya Caixeta de. Collection and Characterization of Standardized Dry Extract of the Fruit of Sucupira Pterodon emarginatus, Fabaceae. 2010. 24 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tde/2095 |
identifier_str_mv |
OLIVEIRA, Patrícya Caixeta de. Collection and Characterization of Standardized Dry Extract of the Fruit of Sucupira Pterodon emarginatus, Fabaceae. 2010. 24 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2010. |
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http://repositorio.bc.ufg.br/tede/handle/tde/2095 |
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Universidade Federal de Goiás |
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Ciências da Saúde - Farmácia |
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Universidade Federal de Goiás |
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