Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Oliveira, Isabelly Santos Rosado de lattes
Orientador(a): Faria, Sergio Miana de lattes
Banca de defesa: Jesus, Ederson da Concei??o, Coelho, Marcia Reed Rodrigues, Coelho, Irene da Silva
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncias Ambientais e Florestais
Departamento: Instituto de Florestas
País: Brasil
Palavras-chave em Português:
fixa??o biol?gica do nitrog?nio
Beta-riz?bios
Filogenia
Palavras-chave em Inglês:
Biological Nitrogen Fixation
Beta-rhizobia
Phylogeny
Área do conhecimento CNPq:
Recursos Florestais e Engenharia Florestal
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/4803
Resumo: The sabi? (Mimosa caesalpiinifolia Benth.) is a woody legume species native to the Caatinga, and which has been successfully introduced in other Brazilian biomes, such as the Cerrado and the Atlantic Forest. This species has high potential for several uses such as hedge, wood, and coal, among others. Its successful adaptation to different biomes may be related to its ability to associate symbiotically with nitrogen-fixing bacteria called rhizobia, in particular to beta-rhizobia of the genus Paraburkholderia. In this context, we studied the phylogeny of rhizobia strains that are involved in this symbiosis in the Caatinga and in the Atlantic Forest. The objective of this work was to study the phylogenetic relationships between rhizobia strains from Rio de Janeiro, where sabi? was introduced, and from regions where this legume is native to discover the relationship between the symbiont and the successful introduction of this species into a new environment. The morphological characterization of the isolates was carried out in yeast-mannitol agar and they were authenticated under sterile conditions in a glasshouse. The genotypic characterization of those strains was carried out by BOX-PCR, and representative strains were chosen for sequencing of the 16S rRNA, recA, and nifH genes, followed by phylogenic analysis with bioinformatics programs. The results showed that sabi? was mainly associated with Paraburkholderia sabiae in the Atlantic Forest, besides nodulating with Rhizobium. Paraburkholderia diazotrophica was the predominant symbiont in the Caatinga, with only one isolate from Rio Grande do Norte belonging to P. symbiotica. Both the taxonomic classification and the separation of strains by location were supported by the 16S rRNA, and recA phylogenies. The strains were also separated by location in the nifH phylogeny, however, differently from what was observed with the other genes, P. phymatum was phylogenetically closer to P. diazotrophica than to P. sabiae, indicating a possible horizontal transfer event between the first two species. The results presented herein demonstrate the ability of sabi? to adapt to bacterial populations native to the Southeast.
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spelling Faria, Sergio Miana de612687127-87http://lattes.cnpq.br/2191253156772498Jesus, Ederson da Concei??oJesus, Ederson da Concei??oCoelho, Marcia Reed RodriguesCoelho, Irene da Silva157804717-03http://lattes.cnpq.br/9864846608754227Oliveira, Isabelly Santos Rosado de2021-06-28T20:26:44Z2018-02-26OLIVEIRA, Isabelly Santos Rosado de. Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica. 2018. 47 f.. Disserta??o (Mestrado em Ci?ncias Ambientais e Florestais) - Instituto de Florestas, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2018.https://tede.ufrrj.br/jspui/handle/jspui/4803The sabi? (Mimosa caesalpiinifolia Benth.) is a woody legume species native to the Caatinga, and which has been successfully introduced in other Brazilian biomes, such as the Cerrado and the Atlantic Forest. This species has high potential for several uses such as hedge, wood, and coal, among others. Its successful adaptation to different biomes may be related to its ability to associate symbiotically with nitrogen-fixing bacteria called rhizobia, in particular to beta-rhizobia of the genus Paraburkholderia. In this context, we studied the phylogeny of rhizobia strains that are involved in this symbiosis in the Caatinga and in the Atlantic Forest. The objective of this work was to study the phylogenetic relationships between rhizobia strains from Rio de Janeiro, where sabi? was introduced, and from regions where this legume is native to discover the relationship between the symbiont and the successful introduction of this species into a new environment. The morphological characterization of the isolates was carried out in yeast-mannitol agar and they were authenticated under sterile conditions in a glasshouse. The genotypic characterization of those strains was carried out by BOX-PCR, and representative strains were chosen for sequencing of the 16S rRNA, recA, and nifH genes, followed by phylogenic analysis with bioinformatics programs. The results showed that sabi? was mainly associated with Paraburkholderia sabiae in the Atlantic Forest, besides nodulating with Rhizobium. Paraburkholderia diazotrophica was the predominant symbiont in the Caatinga, with only one isolate from Rio Grande do Norte belonging to P. symbiotica. Both the taxonomic classification and the separation of strains by location were supported by the 16S rRNA, and recA phylogenies. The strains were also separated by location in the nifH phylogeny, however, differently from what was observed with the other genes, P. phymatum was phylogenetically closer to P. diazotrophica than to P. sabiae, indicating a possible horizontal transfer event between the first two species. The results presented herein demonstrate the ability of sabi? to adapt to bacterial populations native to the Southeast.O sabi? (Mimosa caesalpiinifolia Benth.) ? uma esp?cie lenhosa da fam?lia Fabaceae (Leguminosae) nativa do bioma Caatinga e que foi introduzida com sucesso em outros biomas, como o Cerrado e a Mata Atl?ntica. Esta esp?cie possui alto potencial para usos como: cerca viva, lenha, carv?o entre outras utilidades. O sucesso na adapta??o em diferentes biomas brasileiros pode estar relacionado com a capacidade dessa esp?cie em se associar simbioticamente com bact?rias fixadoras de nitrog?nio, chamadas genericamente de riz?bios, em especial aos beta-riz?bios do g?nero Paraburkholderia. Dentro desse contexto, estudou-se a filogenia de estirpes de bact?rias que estariam envolvidas nessa simbiose nos biomas Caatinga e Mata Atl?ntica. O objetivo deste trabalho foi estudar as rela??es filogen?ticas entre as estirpes de riz?bios provenientes do estado do Rio de Janeiro e de regi?es de onde o sabi? ? nativo, de modo a desvendar a rela??o entre o simbionte e a introdu??o bem-sucedida da esp?cie em outros ambientes. Foram selecionados isolados que foram cultivados e caracterizados morfologicamente em meio 79 e autenticados em casa de vegeta??o em condi??es est?reis. Em seguida, foi realizada a caracteriza??o molecular por BOX-PCR, o sequenciamento dos genes 16S rRNA, recA e nifH e a constru??o das ?rvores filogen?ticas por meio de programas de bioinform?tica. Os resultados mostraram que o sabi? se associou preferencialmente a bact?ria Paraburkholderia sabiae em solos do bioma Mata atl?ntica, al?m de nodular com bact?rias do g?nero Rhizobium. J?, em seu bioma de origem, o simbionte encontrado foi predominantemente a Paraburkholderia diazotrophica; apenas um isolado proveniente do Rio Grande do Norte, foi classificado como Paraburkholderia symbiotica. A classifica??o taxon?mica e a divis?o baseada na localidade foram suportadas tanto pela filogenia do gene 16S rRNA quanto pela do gene recA. A filogenia do gene nifH tamb?m separou os isolados de acordo com seu local de origem. Contudo, ao contr?rio do que foi observado para os demais genes, a P. phymatum se apresentou evolutivamente mais pr?xima de P. diazotrophica do que de P. sabiae, indicando a ocorr?ncia de uma transfer?ncia horizontal desse gene entre as duas primeiras esp?cies. Os resultados aqui apresentados demonstram a capacidade de adapta??o da esp?cie a popula??es bacterianas nativas do Sudeste do Brasil.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2021-06-28T20:26:44Z No. of bitstreams: 1 2018 - Isabelly Santos Rosado de Oliveira.pdf: 1145495 bytes, checksum: 6fdcfa0847757ea9bcf7c460042133b2 (MD5)Made available in DSpace on 2021-06-28T20:26:44Z (GMT). No. of bitstreams: 1 2018 - Isabelly Santos Rosado de Oliveira.pdf: 1145495 bytes, checksum: 6fdcfa0847757ea9bcf7c460042133b2 (MD5) Previous issue date: 2018-02-26Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil.application/pdfhttps://tede.ufrrj.br/retrieve/65751/2018%20-%20Isabelly%20Santos%20Rosado%20de%20Oliveira.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Ambientais e FlorestaisUFRRJBrasilInstituto de FlorestasALLEN, E.K. & ALLEN, O.N. The Leguminosae: A Source Book of Characteristics Uses and Nodulation. 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dc.title.por.fl_str_mv Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
dc.title.alternative.eng.fl_str_mv Isabelly Santos Rosado. Biogeography of rhizobia of sabi? (Mimosa caesalpiniifolia Benth.) isolated from the biomes Caatinga and Atlantic Forest.
title Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
spellingShingle Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
Oliveira, Isabelly Santos Rosado de
fixa??o biol?gica do nitrog?nio
Beta-riz?bios
Filogenia
Biological Nitrogen Fixation
Beta-rhizobia
Phylogeny
Recursos Florestais e Engenharia Florestal
title_short Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
title_full Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
title_fullStr Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
title_full_unstemmed Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
title_sort Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica
author Oliveira, Isabelly Santos Rosado de
author_facet Oliveira, Isabelly Santos Rosado de
author_role author
dc.contributor.advisor1.fl_str_mv Faria, Sergio Miana de
dc.contributor.advisor1ID.fl_str_mv 612687127-87
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2191253156772498
dc.contributor.advisor-co1.fl_str_mv Jesus, Ederson da Concei??o
dc.contributor.referee1.fl_str_mv Jesus, Ederson da Concei??o
dc.contributor.referee2.fl_str_mv Coelho, Marcia Reed Rodrigues
dc.contributor.referee3.fl_str_mv Coelho, Irene da Silva
dc.contributor.authorID.fl_str_mv 157804717-03
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9864846608754227
dc.contributor.author.fl_str_mv Oliveira, Isabelly Santos Rosado de
contributor_str_mv Faria, Sergio Miana de
Jesus, Ederson da Concei??o
Jesus, Ederson da Concei??o
Coelho, Marcia Reed Rodrigues
Coelho, Irene da Silva
dc.subject.por.fl_str_mv fixa??o biol?gica do nitrog?nio
Beta-riz?bios
Filogenia
topic fixa??o biol?gica do nitrog?nio
Beta-riz?bios
Filogenia
Biological Nitrogen Fixation
Beta-rhizobia
Phylogeny
Recursos Florestais e Engenharia Florestal
dc.subject.eng.fl_str_mv Biological Nitrogen Fixation
Beta-rhizobia
Phylogeny
dc.subject.cnpq.fl_str_mv Recursos Florestais e Engenharia Florestal
description The sabi? (Mimosa caesalpiinifolia Benth.) is a woody legume species native to the Caatinga, and which has been successfully introduced in other Brazilian biomes, such as the Cerrado and the Atlantic Forest. This species has high potential for several uses such as hedge, wood, and coal, among others. Its successful adaptation to different biomes may be related to its ability to associate symbiotically with nitrogen-fixing bacteria called rhizobia, in particular to beta-rhizobia of the genus Paraburkholderia. In this context, we studied the phylogeny of rhizobia strains that are involved in this symbiosis in the Caatinga and in the Atlantic Forest. The objective of this work was to study the phylogenetic relationships between rhizobia strains from Rio de Janeiro, where sabi? was introduced, and from regions where this legume is native to discover the relationship between the symbiont and the successful introduction of this species into a new environment. The morphological characterization of the isolates was carried out in yeast-mannitol agar and they were authenticated under sterile conditions in a glasshouse. The genotypic characterization of those strains was carried out by BOX-PCR, and representative strains were chosen for sequencing of the 16S rRNA, recA, and nifH genes, followed by phylogenic analysis with bioinformatics programs. The results showed that sabi? was mainly associated with Paraburkholderia sabiae in the Atlantic Forest, besides nodulating with Rhizobium. Paraburkholderia diazotrophica was the predominant symbiont in the Caatinga, with only one isolate from Rio Grande do Norte belonging to P. symbiotica. Both the taxonomic classification and the separation of strains by location were supported by the 16S rRNA, and recA phylogenies. The strains were also separated by location in the nifH phylogeny, however, differently from what was observed with the other genes, P. phymatum was phylogenetically closer to P. diazotrophica than to P. sabiae, indicating a possible horizontal transfer event between the first two species. The results presented herein demonstrate the ability of sabi? to adapt to bacterial populations native to the Southeast.
publishDate 2018
dc.date.issued.fl_str_mv 2018-02-26
dc.date.accessioned.fl_str_mv 2021-06-28T20:26:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Isabelly Santos Rosado de. Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica. 2018. 47 f.. Disserta??o (Mestrado em Ci?ncias Ambientais e Florestais) - Instituto de Florestas, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2018.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/4803
identifier_str_mv OLIVEIRA, Isabelly Santos Rosado de. Biogeografia de riz?bios de sabi? (Mimosa caesalpiniifolia Benth.) isolados dos biomas Caatinga e Mata Atl?ntica. 2018. 47 f.. Disserta??o (Mestrado em Ci?ncias Ambientais e Florestais) - Instituto de Florestas, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2018.
url https://tede.ufrrj.br/jspui/handle/jspui/4803
dc.language.iso.fl_str_mv por
language por
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