Painel imuno-histoquímico de discos articulares degenerados de pacientes com osteoartrite da articulação temporomandibular

• Ano de defesa: 2019
• Autor(a) principal: Fernandes, Bruno Viezzer
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Articulação temporomandibular; Colágeno; Osteoartrite; Imuno-Histoquímica; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2113

Temporomandibular joint osteoarthritis (TMJOA) is a progressive degenerative disease caused by imbalance between anabolic and catabolic stimuli. The aim of this study was to evaluate histopathological changes, collagen degeneration, and the expression of eleven TMJOA biomarkers in articular discs. Specimens were obtained from patients submitted to discectomy. Discs were divided into anterior band (AB), intermediate zone (IZ), and posterior band (PB) for computerized histomorphometric analyses. Each was assigned a histopathological degeneration score (HDS). Collagen degeneration was assessed with Picrosirius-polarization method. Biomarkers were evaluated through immunohistochemistry, including IGF-1, OPG, VEGF, TNF-α, FGF-23, IHH, MMP-3, MMP-9, TGF-β1, BMP-2 and WNT-3. Image processing softwares were used to calculate average immature collagen ratios and immunostained areas. Spearman rank tests were applied to verify correlations, with significance level of 0.05. Eight degenerated discs from female patients were included. The HDS showed negative correlation with expression of VEGF in IZ and PB (P < 0.05), and positive with TNF-α in AB (P < 0.01). Collagen degeneration correlated with TGF-β1 (P < 0.05), BMP-2 (P < 0.01) and IHH (P < 0.05) immunostained areas in the IZ; TGF-β1, BMP-2 and IHH expression correlated among each other in AB and IZ (P < 0.05). Angiogenesis and tissue fragmentation may result from aberrant physiologic responses mediated by VEGF and TNF-α, compromising TMJ discs during OA progression. The expression of TGF-β1, BMP-2 and IHH could be related to collagen degeneration in displaced discs and may participate in TMJOA pathogenesis.