Avaliação da influência da suplementação de melatonina no controle glicêmico e da obesidade em ratos Wistar obesos e diabéticos

• Ano de defesa: 2019
• Autor(a) principal: Patta, Thiago
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Biotecnologia Industrial; UP
• Palavras-chave em Português: Melatonina; Obesidade; Diabetes mellitus; Síndrome metabólica; Estudo experimental; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2280

The increase in obesity and type 2 diabetes mellitus (T2DM) worldwide has been increasing. Impact directly on the quality and life expectancy of people, associated with the great economic and social impact caused by this epidemic. One of the research lines is the use of melatonin, known as sleep hormone because of its intimate relation to the circadian cycle, because of its positive neuroendocrine effects on the body energy balance of carbohydrates or lipids. The dissertation developed below was composed of two chapters. The first one refers to the literature review on the application and influence of melatonin on obesity and DMT2. The second refers to the experimental study with 16 obese and diabetic wistars rats, which evaluated the influence of melatonin supplementation on glycemic control and obesity. This experiment was composed of three stages: pre-induction phase (IPF) of obesity, which lasted for about 4 weeks, aimed at the weaning of the animals, followed by the induction phase (FI) of obesity with hypercaloric diet lasting 24 weeks and the experimental phase (FE) where the animals were randomly divided and formed the control group (GC) and melatonin group (GM), which had the supplementation of melatonin in nocturnal water at a dose of 20 mg / kg / day with a duration of 8 weeks . During the IF, all the animals evolved with obesity and hyperglycemia, diagnosed through the Lee index and glucose tolerance test (TTGo). At this time separated into equal groups and GM supplemented with melatonin. Results: Melatonin supplementation had a positive influence on weight control in which GM animals decreased, food intake, waist circumference, and consequently the Lee index. In the control of diabetes, the melatonin effect was more timid or even non-existent in some parameters, such as fasting blood glucose and insulin levels. Only when we analyzed the Area Under the Curve (AUC) of the glycemic curves can we observe a slight improvement in GM, not presenting statistical significance. The influence of melatonin supplementation was shown to be effective in combating obesity in GM when compared to CG. The effect on diabetes was unobtrusive, perhaps due to the limited number of animals, short time of the experiment or even dose of melatonin used. Hence the importance of new studies in this line of research.