Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1

Detalhes bibliográficos
Ano de defesa: 2019
Autor(a) principal: Suzuki, Suelen Luiz Gibellato
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Positivo
Brasil
Pós-Graduação
Programa de Pós-Graduação em Biotecnologia Industrial
UP
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Biotecnologia industrial
Solo
Paecilomyces
Zea mays
Aflatoxina B1
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2282
Resumo: Mycotoxins are secondary metabolites produced by filamentous fungi that have toxigenic effects on humans and animals. Considering the contamination of food - especially grains - by this toxin, the need to produce safe food, minimize economic losses related to food contamination, and the absence of effective removal strategies for these contaminants, it is necessary to research and develop processes that effectively act on this issue. In this work the objective was to recover and identify microorganisms with aflatoxin B1 (AFB1) degradation potential from soil and corn (Zea mays) samples submitted to enrichment in coumarin medium, since aflatoxins are derived from difuranocoumarin. Five samples of soil cultivated with soybeans with a recent aflatoxin contamination history, and four samples of corn grains out of quality standards were collected, which were cultivated in a liquid medium containing coumarin as sole carbon source. Enrichment was conducted for six weeks, and at the end of the period only one isolate (S1) with growth capacity in coumarin medium was identified. Isolate S1 was identified by morphological and molecular analysis, which indicated that it is an organism of the genus Paecilomyces sp. In addition, the growth potential of the fungus in coumarincontaining medium as sole carbon source and in nutrient broth added from AFB1 was verified. Further studies should be conducted to evaluate its degradation capacity of AFB1 and to investigate the biotechnological application potential of this isolate
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spelling Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1Biotecnologia industrialSoloPaecilomycesZea maysAflatoxina B1CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALMycotoxins are secondary metabolites produced by filamentous fungi that have toxigenic effects on humans and animals. Considering the contamination of food - especially grains - by this toxin, the need to produce safe food, minimize economic losses related to food contamination, and the absence of effective removal strategies for these contaminants, it is necessary to research and develop processes that effectively act on this issue. In this work the objective was to recover and identify microorganisms with aflatoxin B1 (AFB1) degradation potential from soil and corn (Zea mays) samples submitted to enrichment in coumarin medium, since aflatoxins are derived from difuranocoumarin. Five samples of soil cultivated with soybeans with a recent aflatoxin contamination history, and four samples of corn grains out of quality standards were collected, which were cultivated in a liquid medium containing coumarin as sole carbon source. Enrichment was conducted for six weeks, and at the end of the period only one isolate (S1) with growth capacity in coumarin medium was identified. Isolate S1 was identified by morphological and molecular analysis, which indicated that it is an organism of the genus Paecilomyces sp. In addition, the growth potential of the fungus in coumarincontaining medium as sole carbon source and in nutrient broth added from AFB1 was verified. Further studies should be conducted to evaluate its degradation capacity of AFB1 and to investigate the biotechnological application potential of this isolateAs micotoxinas são metabólitos secundários produzidos por fungos filamentosos que apresentam efeitos toxigênicos para os humanos e animais. Considerando a contaminação dos alimentos – especialmente grãos – por essa toxina, a necessidade de se produzir alimentos seguros, de minimizar as perdas econômicas relacionadas à contaminação dos alimentos, e a ausência de estratégias eficazes de remoção desses contaminantes, faz-se necessária a pesquisa e o desenvolvimento de processos que efetivamente atuem nessa questão. Neste trabalho o objetivo foi recuperar e identificar microrganismos com potencial de degradação de aflatoxina B1 (AFB1) a partir de amostras de solo e de grãos de milho (Zea mays) submetidas ao enriquecimento em meio com cumarina, visto que as aflatoxinas são derivadas da difuranocumarina. Foram coletadas cinco amostras de solo cultivado com soja com histórico recente de contaminação por aflatoxinas, e quatro amostras de grãos de milho fora do padrão de qualidade, as quais foram cultivadas em meio líquido contendo cumarina como única fonte de carbono. O enriquecimento foi conduzido durante seis semanas, e ao final do período foi identificado apenas um isolado (S1) com capacidade de crescimento no meio cumarina. O isolado S1 foi identificado por análises morfológica e molecular, que indicaram tratar-se de um organismo do gênero Paecilomyces sp. Além disso, foi constatado o potencial de crescimento do fungo em meio contendo cumarina como única fonte de carbono e em caldo nutriente adicionado de AFB1. Estudos posteriores deverão ser realizados com a finalidade de avaliar sua capacidade de degradação de AFB1 e investigar o potencial de aplicação de biotecnológica deste isoladoUniversidade PositivoBrasilPós-GraduaçãoPrograma de Pós-Graduação em Biotecnologia IndustrialUPAlvarez, Thabata Mariahttp://lattes.cnpq.br/5098256501937905Suzuki, Suelen Luiz Gibellato2021-05-27T20:00:30Z20192021-05-27T20:00:30Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/2282porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2021-05-28T15:21:05Zoai:repositorio.cruzeirodosul.edu.br:123456789/2282Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2021-05-28T15:21:05Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false
dc.title.none.fl_str_mv Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
title Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
spellingShingle Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
Suzuki, Suelen Luiz Gibellato
Biotecnologia industrial
Solo
Paecilomyces
Zea mays
Aflatoxina B1
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
title_full Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
title_fullStr Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
title_full_unstemmed Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
title_sort Estratégia de enriquecimento para isolamento de microrganismos com potencial para degradação de aflatoxina B1
author Suzuki, Suelen Luiz Gibellato
author_facet Suzuki, Suelen Luiz Gibellato
author_role author
dc.contributor.none.fl_str_mv Alvarez, Thabata Maria
http://lattes.cnpq.br/5098256501937905
dc.contributor.author.fl_str_mv Suzuki, Suelen Luiz Gibellato
dc.subject.por.fl_str_mv Biotecnologia industrial
Solo
Paecilomyces
Zea mays
Aflatoxina B1
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
topic Biotecnologia industrial
Solo
Paecilomyces
Zea mays
Aflatoxina B1
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description Mycotoxins are secondary metabolites produced by filamentous fungi that have toxigenic effects on humans and animals. Considering the contamination of food - especially grains - by this toxin, the need to produce safe food, minimize economic losses related to food contamination, and the absence of effective removal strategies for these contaminants, it is necessary to research and develop processes that effectively act on this issue. In this work the objective was to recover and identify microorganisms with aflatoxin B1 (AFB1) degradation potential from soil and corn (Zea mays) samples submitted to enrichment in coumarin medium, since aflatoxins are derived from difuranocoumarin. Five samples of soil cultivated with soybeans with a recent aflatoxin contamination history, and four samples of corn grains out of quality standards were collected, which were cultivated in a liquid medium containing coumarin as sole carbon source. Enrichment was conducted for six weeks, and at the end of the period only one isolate (S1) with growth capacity in coumarin medium was identified. Isolate S1 was identified by morphological and molecular analysis, which indicated that it is an organism of the genus Paecilomyces sp. In addition, the growth potential of the fungus in coumarincontaining medium as sole carbon source and in nutrient broth added from AFB1 was verified. Further studies should be conducted to evaluate its degradation capacity of AFB1 and to investigate the biotechnological application potential of this isolate
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2021-05-27T20:00:30Z
2021-05-27T20:00:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.cruzeirodosul.edu.br/handle/123456789/2282
url https://repositorio.cruzeirodosul.edu.br/handle/123456789/2282
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Positivo
Brasil
Pós-Graduação
Programa de Pós-Graduação em Biotecnologia Industrial
UP
publisher.none.fl_str_mv Universidade Positivo
Brasil
Pós-Graduação
Programa de Pós-Graduação em Biotecnologia Industrial
UP
dc.source.none.fl_str_mv reponame:Repositório do Centro Universitário Braz Cubas
instname:Centro Universitário Braz Cubas (CUB)
instacron:CUB
instname_str Centro Universitário Braz Cubas (CUB)
instacron_str CUB
institution CUB
reponame_str Repositório do Centro Universitário Braz Cubas
collection Repositório do Centro Universitário Braz Cubas
repository.name.fl_str_mv Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)
repository.mail.fl_str_mv bibli@brazcubas.edu.br
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