Caracterização genotípica e fenotípica de Candida sp de fluido vaginal de mulheres adultas

• Ano de defesa: 2010
• Autor(a) principal: Cunha, Keith Cássia da
• Orientador(a): Almeida, Margarete Teresa Gotardo de
• Banca de defesa: Bagarelli, Lucia Buchalla , Almeida, Ana Marisa Fusco
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Faculdade de Medicina de São José do Rio Preto
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências da Saúde
• Departamento: Medicina Interna; Medicina e Ciências Correlatas
• País: BR
• Palavras-chave em Português: Candida sp; RAPD-PCR; Candidíase Vulvovaginal
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::GINECOLOGIA E OBSTETRICIA
• Link de acesso: http://bdtd.famerp.br/handle/tede/75

Candida albicans is widely recognized as the pathogen often occurring in vulvovaginal candidiasis. Objective: To compare the genotypes, among infection or colonization and recurrent isolates, with virulence determinants (phospholipase, proteinase, amylase, gelatinase, haemolysin, thermotolerance and biofilm), and to in vitro antifungal susceptibilities. Methods: 317 samples of vaginal fluid of were collected from women with or without symptomatic indications to VVC, including vaginal itching, abnormal discharge, soreness and dyspareunia to mycological investigation. The antimicrobial susceptibility was evaluated for 4 drugs (fluconazole, Itraconazole, ketoconazole and amphotericin B, by dilution method (M27A2- CLSI). Yeasts were subjected PCR (polimerase chain reaction), RAPD (randomly amplified polymorphic DNA), and to RFLP (Restriction fragment lenght polymorphic), this last one, used for identification of Candida dubliniensis. Results: Considering the prevalent species, C. albicans showed high rates of significant sensitivity to azoles and the vast majority of produced virulence factors, regardless of their origin, infection or colonization. Statistical analysis enabled identification of 17 clusters with identical genetic identity, and other moderately related, or unrelated. Conclusions: There was no correlation between the genetic patterns with virulence and antimicrobial susceptibility for all isolates, suggesting that, the amplified genetic regions do not match with variables and might be related to other biological events. The inclusion of other primers could allow higher association among C. albicans strains.