Valida??o da prote?na MDP2 de Mycobacterium tuberculosis H37Rv como uma prote?na intrinsecamente desordenada e gera??o de uma cepa nocaute para o gene hns

• Ano de defesa: 2014
• Autor(a) principal: Abbadi, Bruno Lopes
• Orientador(a): Bizarro, Cristiano Valim
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontif?cia Universidade Cat?lica do Rio Grande do Sul
• Programa de Pós-Graduação: Programa de P?s-Gradua??o em Biologia Celular e Molecular
• Departamento: Faculdade de Bioci?ncias
• País: BR
• Palavras-chave em Português: BIOLOGIA CELULAR; TUBERCULOSE; INFEC??O; ESPECTROMETRIA; PROTE?NAS VIRAIS
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://tede2.pucrs.br/tede2/handle/tede/5491

The mycobacterial DNA-binding protein 2 (MDP2) of Mycobacterium tuberculosis is a small, basic protein known to bind to DNA in a non-specific manner and to anchor the nucleoid to the plasma membrane, promoting its unpacking. Motivated by a prior intrinsic disorder pre-diction in silico, we have used a complementary set of techniques to characterize this pro-tein, such as heat and chemical stability, gel filtration, limited proteolysis and electrophoret-ic mobility. Our results suggest that the MDP2 is structurally disordered, since it (1) was pre-dicted to be an IDP, having 86 % of its structure disordered; (2) resisted to denaturation in-duced by boiling temperature and to the chemical thrichloroacetic acid (TCA); (3) migrated anomalously on SDS-PAGE, appearing to be 1.4-fold higher its calculated molecular weight; and (4) was highly sensitive to proteolysis performed by proteinase K in comparison to glob-ular proteins. We also accomplished two experiments to determine the quaternary structure of the MDP2, such as gel-filtration and glutaraldehyde crosslinking. These two experiments showed that the protein has a tendency of self-aggregation, forming large clusters of pro-tein. We also performed the site-directed mutagenesis by allelic exchange in the hns gene, in order to develop a M. tuberculosis strain lacking the protein MDP2. The result of this knock-out showed that the hns gene is not essential for the survival of the mycobacteria, confirm-ing previous results performed by transposon mutagenesis. Combined with previous results related to MDP2 from other works, we speculate that this protein uses its highly disorder N-terminal region to bind to DNA through its KAAK and PAKK sequences in a non-specific man-ner. Thereby the MDP2 may act as a promiscuous protein inside the cell, binding to different regions of nucleoid by forming large clusters of proteins, in order to perform the DNA un-packing and to regulate several genes of the mycobacteria. We hope this work may contrib-ute to a better understanding of the mycobacterial metabolism, since the M. tuberculosis still stands a major global threat.