Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade
Ano de defesa: | 2018 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Gerontologia Biom?dica
|
Departamento: |
Escola de Medicina
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/8890 |
Resumo: | Fear memories can lead to defensive behavior in response to threats, which can be modulated by different drugs or exposure to a novelty, for example. The histaminergic, serotonergic and adrenergic systems have receptors located in several areas in the brain, which are involved in the process of extinction memory. It is known that different processes can modulate the extinction memory, among them is the exposure to a novelty, which facilitates the formation of extinction memory. Here we investigate the participation of the H2-histaminergic, 5-HT1A serotonergic and ?-adrenergic receptors of the hippocampus on the enhancement of extinction of fear memory by exposure to novelty. For this, male Wistar rats (300-330 g) were submitted to a stereotaxic surgery to implant guide cannula into the CA1 region of hippocampus. After 7 days of recovery, the animals were submitted to the experimental protocols (contextual fear conditioning with or without exposure to a novelty). The intra-CA1 infusion immediately after exposure to novelty or after extinction of the histaminergic H2 receptors agonist, Dimaprit (2,3 ?g per side) or the antagonist, Ranitidine (17,5 ?g per side) had no effect on the facilitation of extinction by exposure to novelty, suggesting that this receptor is not necessary in this process. It was also observed that the administration of 5-HT1A serotonergic receptor agonist, 8-OH-DPAT (6.25 ?g per side) and of the blocker of ?-adrenergic receptor, Timolol (1.0 ?g per side) impaired the enhancement of the extinction memory induced by exposure to a novelty, thus suggesting that these receptors are involved in the process of facilitation of extinction by exposure to novelty. Thus, the results suggest that the 5-HT1A serotonergic and ?-adrenergic receptors, but not H2-histaminergic receptors, play a role in the enhancement of extinction induced by novelty, indicating a complex regulation of the novelty effect by some, but not all, important neurotransmitter systems. |
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Myskiw, Jociane de Carvalhohttp://lattes.cnpq.br/3146559515066831http://lattes.cnpq.br/5501465951755277Nachtigall, Eduarda Godfried2019-09-19T19:36:24Z2018-02-28http://tede2.pucrs.br/tede2/handle/tede/8890Fear memories can lead to defensive behavior in response to threats, which can be modulated by different drugs or exposure to a novelty, for example. The histaminergic, serotonergic and adrenergic systems have receptors located in several areas in the brain, which are involved in the process of extinction memory. It is known that different processes can modulate the extinction memory, among them is the exposure to a novelty, which facilitates the formation of extinction memory. Here we investigate the participation of the H2-histaminergic, 5-HT1A serotonergic and ?-adrenergic receptors of the hippocampus on the enhancement of extinction of fear memory by exposure to novelty. For this, male Wistar rats (300-330 g) were submitted to a stereotaxic surgery to implant guide cannula into the CA1 region of hippocampus. After 7 days of recovery, the animals were submitted to the experimental protocols (contextual fear conditioning with or without exposure to a novelty). The intra-CA1 infusion immediately after exposure to novelty or after extinction of the histaminergic H2 receptors agonist, Dimaprit (2,3 ?g per side) or the antagonist, Ranitidine (17,5 ?g per side) had no effect on the facilitation of extinction by exposure to novelty, suggesting that this receptor is not necessary in this process. It was also observed that the administration of 5-HT1A serotonergic receptor agonist, 8-OH-DPAT (6.25 ?g per side) and of the blocker of ?-adrenergic receptor, Timolol (1.0 ?g per side) impaired the enhancement of the extinction memory induced by exposure to a novelty, thus suggesting that these receptors are involved in the process of facilitation of extinction by exposure to novelty. Thus, the results suggest that the 5-HT1A serotonergic and ?-adrenergic receptors, but not H2-histaminergic receptors, play a role in the enhancement of extinction induced by novelty, indicating a complex regulation of the novelty effect by some, but not all, important neurotransmitter systems.As mem?rias de medo podem levar a comportamentos defensivos em resposta a amea?as, as quais podem ser moduladas por diferentes drogas ou exposi??o a uma novidade, por exemplo. O sistema histamin?rgico, serotonin?rgico e noradren?rgico possurem receptores localizados em diversas ?reas do c?rebro, e estes participam no processo de extin??o da mem?ria. Recentemente, nosso grupo demonstrou que animais expostos a uma novidade 1 e 2 h antes ou 1 h ap?s uma sess?o fraca de extin??o que n?o expressavam a mem?ria de extin??o passavam a expressar. Portanto, este estudo teve como objetivo investigar a participa??o dos receptores H2 histamin?rgicos, 5-HT1A serotonin?rgicos e ?-adren?rgicos, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade. Para isso, ratos Wistar machos (300-330 gramas) foram submetidos a uma cirurgia estereot?xica para implante bilateral de c?nulas guia na regi?o CA1 do hipocampo. Ap?s 7 dias de recupera??o, os animais foram submetidos aos protocolos experimentais (medo condicionado ao contexto com ou sem exposi??o a uma novidade). Verificou-se que as infus?es intra-CA1 imediatamente ap?s a exposi??o a novidade ou ap?s sess?o de extin??o, do agonista Dimaprit (2,3 ?g/lado) ou antagonista Ranitidina (17,5 ?g/lado) dos receptores H2 histamin?rgicos n?o afetaram a facilita??o da extin??o pela exposi??o a novidade, sugerindo que este receptor n?o ? necess?rio neste processo. Observou-se tamb?m que quando administrado o agonista do receptor 5-HT1A serotonin?rgico, 8-OH-DPAT (6,25?g/lado) e quando bloqueado o receptor ?-adren?rgico pelo antagonista Timolol (1,0 ?g/lado) houve um preju?zo da facilita??o da forma??o da mem?ria de extin??o pela exposi??o a uma novidade, sugerindo assim que esses receptores est?o envolvidos no processo de forma??o da extin??o da mem?ria de medo condicionada ao contexto. Assim, os resultados obtidos sugerem que os receptores 5-HT1A serotonin?rgicos e ?-adren?rgicos, mas n?o os H2 histamin?rgicos, participam na facilita??o da forma??o da extin??o da mem?ria de medo condicionada ao contexto pela exposi??o a uma novidade, indicando uma regula??o complexa do efeito novidade por alguns, mas n?o todos, importantes sistemas de neurotransmissores.Submitted by PPG Gerontologia Biom?dica (geronbio@pucrs.br) on 2019-09-10T18:14:08Z No. of bitstreams: 1 NACHTIGALL_ EDUARDA_GODFRIED_DIS.pdf: 1152546 bytes, checksum: 13b2194ed72f6759b2804f7bd80173b0 (MD5)Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2019-09-19T19:20:23Z (GMT) No. of bitstreams: 1 NACHTIGALL_ EDUARDA_GODFRIED_DIS.pdf: 1152546 bytes, checksum: 13b2194ed72f6759b2804f7bd80173b0 (MD5)Made available in DSpace on 2019-09-19T19:36:24Z (GMT). No. of bitstreams: 1 NACHTIGALL_ EDUARDA_GODFRIED_DIS.pdf: 1152546 bytes, checksum: 13b2194ed72f6759b2804f7bd80173b0 (MD5) Previous issue date: 2018-02-28Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/176424/NACHTIGALL_%20EDUARDA_GODFRIED_DIS.pdf.jpghttp://tede2.pucrs.br:80/tede2/retrieve/176783/DIS_EDUARDA_GODFRIED_NACHTIGALL_CONFIDENCIAL.pdf.jpghttp://tede2.pucrs.br:80/tede2/retrieve/179087/DIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Gerontologia Biom?dicaPUCRSBrasilEscola de MedicinaExtin??oMedo Condicionado ao ContextoHipocampoSistema Modulat?rioNovidadeBehavioral TaggingCIENCIAS DA SAUDE::MEDICINAParticipa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidadeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTrabalho ser? publicado como artigo ou livro12 meses19/09/20208969645070886364160500600500600-224747486637135387-9693694523087866273590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSORIGINALDIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdfDIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdfapplication/pdf1152546http://tede2.pucrs.br/tede2/bitstream/tede/8890/8/DIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf13b2194ed72f6759b2804f7bd80173b0MD58THUMBNAILNACHTIGALL_ EDUARDA_GODFRIED_DIS.pdf.jpgNACHTIGALL_ EDUARDA_GODFRIED_DIS.pdf.jpgimage/jpeg5961http://tede2.pucrs.br/tede2/bitstream/tede/8890/4/NACHTIGALL_+EDUARDA_GODFRIED_DIS.pdf.jpg465d38a8db0ec161b0a34030a905e5dbMD54DIS_EDUARDA_GODFRIED_NACHTIGALL_CONFIDENCIAL.pdf.jpgDIS_EDUARDA_GODFRIED_NACHTIGALL_CONFIDENCIAL.pdf.jpgimage/jpeg4085http://tede2.pucrs.br/tede2/bitstream/tede/8890/7/DIS_EDUARDA_GODFRIED_NACHTIGALL_CONFIDENCIAL.pdf.jpg585ded4cca01c91c7932d2022eddd897MD57DIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf.jpgDIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf.jpgimage/jpeg5961http://tede2.pucrs.br/tede2/bitstream/tede/8890/10/DIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf.jpg465d38a8db0ec161b0a34030a905e5dbMD510TEXTNACHTIGALL_ EDUARDA_GODFRIED_DIS.pdf.txtNACHTIGALL_ EDUARDA_GODFRIED_DIS.pdf.txttext/plain127219http://tede2.pucrs.br/tede2/bitstream/tede/8890/3/NACHTIGALL_+EDUARDA_GODFRIED_DIS.pdf.txt617ee46764396b1b294f6e587a6a6693MD53DIS_EDUARDA_GODFRIED_NACHTIGALL_CONFIDENCIAL.pdf.txtDIS_EDUARDA_GODFRIED_NACHTIGALL_CONFIDENCIAL.pdf.txttext/plain2199http://tede2.pucrs.br/tede2/bitstream/tede/8890/6/DIS_EDUARDA_GODFRIED_NACHTIGALL_CONFIDENCIAL.pdf.txtacd21efa45c7347a7141b09699ab8dfaMD56DIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf.txtDIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf.txttext/plain127219http://tede2.pucrs.br/tede2/bitstream/tede/8890/9/DIS_EDUARDA_GODFRIED_NACHTIGALL_COMPLETO.pdf.txt617ee46764396b1b294f6e587a6a6693MD59LICENSElicense.txtlicense.txttext/plain; charset=utf-8590http://tede2.pucrs.br/tede2/bitstream/tede/8890/1/license.txt220e11f2d3ba5354f917c7035aadef24MD51tede/88902020-10-22 13:00:17.574oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2020-10-22T15:00:17Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade |
title |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade |
spellingShingle |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade Nachtigall, Eduarda Godfried Extin??o Medo Condicionado ao Contexto Hipocampo Sistema Modulat?rio Novidade Behavioral Tagging CIENCIAS DA SAUDE::MEDICINA |
title_short |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade |
title_full |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade |
title_fullStr |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade |
title_full_unstemmed |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade |
title_sort |
Participa??o dos receptores H2 histamin?rgico, 5-Ht1a serotonin?rgicos e ?-adren?rgico, do hipocampo, na facilita??o da extin??o da mem?ria de medo condicionado ao contexto pela exposi??o a uma novidade |
author |
Nachtigall, Eduarda Godfried |
author_facet |
Nachtigall, Eduarda Godfried |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Myskiw, Jociane de Carvalho |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3146559515066831 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5501465951755277 |
dc.contributor.author.fl_str_mv |
Nachtigall, Eduarda Godfried |
contributor_str_mv |
Myskiw, Jociane de Carvalho |
dc.subject.por.fl_str_mv |
Extin??o Medo Condicionado ao Contexto Hipocampo Sistema Modulat?rio Novidade Behavioral Tagging |
topic |
Extin??o Medo Condicionado ao Contexto Hipocampo Sistema Modulat?rio Novidade Behavioral Tagging CIENCIAS DA SAUDE::MEDICINA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Fear memories can lead to defensive behavior in response to threats, which can be modulated by different drugs or exposure to a novelty, for example. The histaminergic, serotonergic and adrenergic systems have receptors located in several areas in the brain, which are involved in the process of extinction memory. It is known that different processes can modulate the extinction memory, among them is the exposure to a novelty, which facilitates the formation of extinction memory. Here we investigate the participation of the H2-histaminergic, 5-HT1A serotonergic and ?-adrenergic receptors of the hippocampus on the enhancement of extinction of fear memory by exposure to novelty. For this, male Wistar rats (300-330 g) were submitted to a stereotaxic surgery to implant guide cannula into the CA1 region of hippocampus. After 7 days of recovery, the animals were submitted to the experimental protocols (contextual fear conditioning with or without exposure to a novelty). The intra-CA1 infusion immediately after exposure to novelty or after extinction of the histaminergic H2 receptors agonist, Dimaprit (2,3 ?g per side) or the antagonist, Ranitidine (17,5 ?g per side) had no effect on the facilitation of extinction by exposure to novelty, suggesting that this receptor is not necessary in this process. It was also observed that the administration of 5-HT1A serotonergic receptor agonist, 8-OH-DPAT (6.25 ?g per side) and of the blocker of ?-adrenergic receptor, Timolol (1.0 ?g per side) impaired the enhancement of the extinction memory induced by exposure to a novelty, thus suggesting that these receptors are involved in the process of facilitation of extinction by exposure to novelty. Thus, the results suggest that the 5-HT1A serotonergic and ?-adrenergic receptors, but not H2-histaminergic receptors, play a role in the enhancement of extinction induced by novelty, indicating a complex regulation of the novelty effect by some, but not all, important neurotransmitter systems. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-02-28 |
dc.date.accessioned.fl_str_mv |
2019-09-19T19:36:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://tede2.pucrs.br/tede2/handle/tede/8890 |
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http://tede2.pucrs.br/tede2/handle/tede/8890 |
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por |
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por |
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8969645070886364160 |
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500 600 500 600 |
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-969369452308786627 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Gerontologia Biom?dica |
dc.publisher.initials.fl_str_mv |
PUCRS |
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Brasil |
dc.publisher.department.fl_str_mv |
Escola de Medicina |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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