Complexos de metais de transição para o desenvolvimento de novas drogas neuroativas

• Ano de defesa: 2010
• Autor(a) principal: Cardoso, Carolina Riverin
• Orientador(a): Carlos, Rose Maria
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Carlos
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química - PPGQ
• País: BR
• Palavras-chave em Português: Química inorgânica; Complexos de rutênio; Fotoquímica; Luminescência; Fármaco
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: https://repositorio.ufscar.br/handle/20.500.14289/6464

The biological role and pharmacological utility of histamine and 4-aminopyridine in the physiological medium has led us to the development of luminescent and photolabile metal-histamine and metal-4-aminopyridine donor that release histamine when exposed to visible light aim both research tools and therapeutic agents. In this work, we report on the synthesis, photochemical and photophysical studies and bovine serum albumin (BSA) binding properties of cis-[Ru(phen)2(L)2]2+, L = histamine (Ru-him) and 4-apy (Ru-4apy). The 1H-NMR experiments and DFT calculations confirm the presence of two linkage isomers (binding through N1 or N3 of histamine) of Ru-him. In CD3CN the Ru- N1(histamine), R isomer, being favored whereas in protic solvents the Ru-N3(histamine), A isomer, is the major isomer. The presence of two isomeric populations is a possible explanation for the biexponential fluorescence decay of Ru-Him (1 = 184.7 ns and 2 = 43.76 ns). The cis-[Ru(phen)2(L)2]2+ are water soluble and highly colored with strong absorptions (~ 490 nm, 13000 mol-1 L cm-1) and a broad emission at 640 nm. They are thermally stable over the course of many hours in non-aqueous and aqueous solution but they are photochemically reactive. Photolysis in the visible absorption band of cis- [Ru(phen)2(L)2]2+ in the CH3CN and CH3OH solution causes an efficient photoreaction, converting them into cis-[Ru(phen)2(CH3CN)2]2+. L photorelease is also observed in pure water and in an aqueous buffer solution (Tris/HCl, pH 7.4) in the presence of BSA (1.0 x 10-6 mol L-1). The cis-[Ru(phen)2(L)2]2+ complex quenches the fluorescence of BSA by a combination of static and dynamic quenching, but no changes in the emission spectrum of BSA were observed when free histamine (2.5 x 10-6 to 5 x 10-5 mol L-1) or free 4- aminopyridine (2.5 x 10-6 to 9 x 10-5 mol L-1) were used as a quencher. This suggests that the [Ru(phen)2]2+ moiety presents structural features which are essential to L - BSA xii interaction. It is of relevance to note that the presence of BSA does not preclude the photorelease of L in complex cis-[Ru(phen)2(L)2]2+. Overall, these results are of particular interest to the design of intelligent metallodrugs based on photochemical and photophysical processes.