DNA livre circulante de células do HPV nas neoplasias HPV relacionadas

• Ano de defesa: 2022
• Autor(a) principal: Oliveira, Pedro Henrique de
• Orientador(a): Vieira, Rosangela Andrade
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Católica de Brasília
• Programa de Pós-Graduação: Programa Stricto Sensu em Ciências Genômicas e Biotecnologia
• Departamento: Escola de Saúde e Medicina
• País: Brasil
• Palavras-chave em Português: Biópsia líquida; DNA livre circulante (cfDNA); Neoplasia relacionada ao papilomavirus humano (HPV)
• Palavras-chave em Inglês: Human papillomavirus (HPV) related neoplasm; Free circulating DNA (cfDNA); Liquid biopsy; PCR
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS
• Link de acesso: https://bdtd.ucb.br:8443/jspui/handle/tede/3327

The patients diagnosed with neoplasia related to human papillomavirus represent an important portion of the total number of cases of diagnosed cancer and need biomarcators for treatment monitoring. The main diagnosis strategies for this neoplasia are invasive and depend on the ability to collect tumoral tissue sam ples from biopsies. Liquid biopsies, however, offer a noninvasive approach and can detect tumoral mo lecular elements with greater facility. Free DNA circulating cell (cfDna) are fragments of degraded DNA released into the blood plasma and can be used to describe several forms of genetic material that circulate freely in the bloodstream and has been used in this type of biopsy. In this way, the great challenge is to patronize a method to quantify the free HPV cell circulating DNA in patients of these neoplasies, the main goal of this job. For this, blood samples of patients with this locally advanced or metastatic disease were collected for HPV's cfDna extraction and quantification through QPCR. The results result in the need for more accurate methodologies and more pure samples for this patronization. Once standardized, this meth odology will favor the monitoring of the clinical progression of patients diagnosed with neoplasia related to human papillomavirus, using less invasive techniques, which will offer a better follow-up of treatment, and progression of the disease and provide a greater quality of life.