Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae)
Ano de defesa: | 2022 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual de Feira de Santana
|
Programa de Pós-Graduação: |
Doutorado Acad?mico em Biotecnologia
|
Departamento: |
DEPARTAMENTO DE CI?NCIAS BIOL?GICAS
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.uefs.br:8080/handle/tede/1502 |
Resumo: | The present study was divided into two chapters. The first chapter proposed to investigate patents related to biological activities of the genus Passiflora, where was observed the great potential of several parts of different species, to obtain phytoproducts. The second chapter proposed to obtain fruit peel extracts of P. cincinnata and to evaluate its hypolipidemic activity in vivo, as important steps for the development of herbal medicines. Thus, were performed physicochemical tests with the vegetable drug, according to Brazilian Pharmacopoeia, analysis of chemical constituents present in extracts, through high-performance liquid chromatography (HPLC-DAD), as well as, evaluation of acute toxicity, hypolipidemic activity of the extract (administered orally: 100 and 200 mg/kg), in induced hyperlipidemic mice, and investigation of the possible mechanism of action, through molecular dynamics simulations. The physicochemical assays were within the pharmacopoeial standards. The presence of the vitexin, orientin and isoorientin was confirmed by HPLC-DAD. No clinical signs of toxicity were observed in the evaluated animals. The hypolipidemic analysis presented a reduction in total cholesterol levels (TC) and an increase in high-density lipoprotein (HDL-c), statistically significant (p < 0.05), when compared to control only induced, while triglycerides indices (TG) did not show a significant response. The analysis of molecular docking showed that the compounds in general showed stability in practically the entire simulation, mainly the vitexin, when associated with the LCAT enzyme, demonstrating that the evaluated extract has lipid-lowering activity, possibly due to the presence of the identified bioactive compounds. |
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Almeida, Jackson Roberto Guedes da Silva03016512483http://lattes.cnpq.br/676274200266025190576985449http://lattes.cnpq.br/3417559216830484Leal, Ana Edil?ia Barbosa Pereira2023-08-08T15:31:22Z2022-03-31LEAL, Ana Edil?ia Barbosa Pereira. Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae). 2022. 104f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana,Feira de Santana, 2022.http://tede2.uefs.br:8080/handle/tede/1502The present study was divided into two chapters. The first chapter proposed to investigate patents related to biological activities of the genus Passiflora, where was observed the great potential of several parts of different species, to obtain phytoproducts. The second chapter proposed to obtain fruit peel extracts of P. cincinnata and to evaluate its hypolipidemic activity in vivo, as important steps for the development of herbal medicines. Thus, were performed physicochemical tests with the vegetable drug, according to Brazilian Pharmacopoeia, analysis of chemical constituents present in extracts, through high-performance liquid chromatography (HPLC-DAD), as well as, evaluation of acute toxicity, hypolipidemic activity of the extract (administered orally: 100 and 200 mg/kg), in induced hyperlipidemic mice, and investigation of the possible mechanism of action, through molecular dynamics simulations. The physicochemical assays were within the pharmacopoeial standards. The presence of the vitexin, orientin and isoorientin was confirmed by HPLC-DAD. No clinical signs of toxicity were observed in the evaluated animals. The hypolipidemic analysis presented a reduction in total cholesterol levels (TC) and an increase in high-density lipoprotein (HDL-c), statistically significant (p < 0.05), when compared to control only induced, while triglycerides indices (TG) did not show a significant response. The analysis of molecular docking showed that the compounds in general showed stability in practically the entire simulation, mainly the vitexin, when associated with the LCAT enzyme, demonstrating that the evaluated extract has lipid-lowering activity, possibly due to the presence of the identified bioactive compounds.O presente estudo foi dividido em dois cap?tulos. O primeiro cap?tulo prop?s investigar patentes relacionadas a atividades biol?gicas do g?nero Passiflora, onde foi observado o grande potencial de v?rias partes de diferentes esp?cies, para a obten??o de fitoprodutos. O segundo cap?tulo prop?s obter extratos de cascas dos frutos de P. cincinnata e avaliar sua atividade hipolipid?mica in vivo, como etapa importante para o desenvolvimento de fitoter?picos. Assim, foram realizados testes f?sico-qu?micos com a droga vegetal, conforme Farmacopeia Brasileira, an?lise de constituintes qu?micos presentes nos extratos, atrav?s de cromatografia l?quida de alta efici?ncia acoplada a detector de arranjo de diodos (CLAE-DAD), assim como avalia??o da toxicidade aguda, atividade hipolipemiante do extrato (administrado por via oral: 100 e 200 mg/kg), em camundongos hiperlipid?micos induzidos, e investiga??o do poss?vel mecanismo de a??o, atrav?s de simula??es de din?mica molecular. Os ensaios f?sico-qu?micos apresentaram-se dentro dos padr?es farmacopeicos. A presen?a de vitexina, orientina e isoorientina foi confirmada por CLAE-DAD. Nenhum sinal cl?nico de toxicidade foi observado nos animais avaliados. A an?lise hipolipid?mica apresentou uma redu??o nos n?veis de colesterol total (CT) e aumento da lipoprote?na de alta densidade (HDL-c), estatisticamente significativos (p < 0,05), quando comparados ao controle apenas induzido, enquanto os ?ndices de triglicer?deos (TG) n?o mostraram uma resposta significativa. A an?lise de docking molecular mostrou que os compostos de um modo geral apresentaram estabilidade em praticamente toda a simula??o, principalmente a vitexina, quando associado ? enzima LCAT, demostrando que o extrato avaliado possui atividade hipolipemiante, possivelmente devido ? presen?a dos compostos bioativos identificados.Submitted by Amanda Ponce (aponce@uefs.br) on 2023-08-08T15:31:22Z No. of bitstreams: 1 TESE_Ana_Edileia_Leal.pdf: 2108553 bytes, checksum: ed3bdc12aa00f9ba9aa0a54b1462319a (MD5)Made available in DSpace on 2023-08-08T15:31:22Z (GMT). No. of bitstreams: 1 TESE_Ana_Edileia_Leal.pdf: 2108553 bytes, checksum: ed3bdc12aa00f9ba9aa0a54b1462319a (MD5) Previous issue date: 2022-03-31application/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acad?mico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CI?NCIAS BIOL?GICASPassifloraPassiflora cincinnataFlavonoides C-glicos?deosHiperlipidemiaFitoter?picosFlavonoids C-glycosidesHyperlipidemiaPhytotherapicCIENCIAS BIOLOGICAS::BIOLOGIA GERALCIENCIAS BIOLOGICAS::FARMACOLOGIAFARMACIA::FARMACOGNOSIAControle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. 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dc.title.por.fl_str_mv |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) |
title |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) |
spellingShingle |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) Leal, Ana Edil?ia Barbosa Pereira Passiflora Passiflora cincinnata Flavonoides C-glicos?deos Hiperlipidemia Fitoter?picos Flavonoids C-glycosides Hyperlipidemia Phytotherapic CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS::FARMACOLOGIA FARMACIA::FARMACOGNOSIA |
title_short |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) |
title_full |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) |
title_fullStr |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) |
title_full_unstemmed |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) |
title_sort |
Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae) |
author |
Leal, Ana Edil?ia Barbosa Pereira |
author_facet |
Leal, Ana Edil?ia Barbosa Pereira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Almeida, Jackson Roberto Guedes da Silva |
dc.contributor.advisor1ID.fl_str_mv |
03016512483 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6762742002660251 |
dc.contributor.authorID.fl_str_mv |
90576985449 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3417559216830484 |
dc.contributor.author.fl_str_mv |
Leal, Ana Edil?ia Barbosa Pereira |
contributor_str_mv |
Almeida, Jackson Roberto Guedes da Silva |
dc.subject.por.fl_str_mv |
Passiflora Passiflora cincinnata Flavonoides C-glicos?deos Hiperlipidemia Fitoter?picos |
topic |
Passiflora Passiflora cincinnata Flavonoides C-glicos?deos Hiperlipidemia Fitoter?picos Flavonoids C-glycosides Hyperlipidemia Phytotherapic CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS::FARMACOLOGIA FARMACIA::FARMACOGNOSIA |
dc.subject.eng.fl_str_mv |
Flavonoids C-glycosides Hyperlipidemia Phytotherapic |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS::FARMACOLOGIA FARMACIA::FARMACOGNOSIA |
description |
The present study was divided into two chapters. The first chapter proposed to investigate patents related to biological activities of the genus Passiflora, where was observed the great potential of several parts of different species, to obtain phytoproducts. The second chapter proposed to obtain fruit peel extracts of P. cincinnata and to evaluate its hypolipidemic activity in vivo, as important steps for the development of herbal medicines. Thus, were performed physicochemical tests with the vegetable drug, according to Brazilian Pharmacopoeia, analysis of chemical constituents present in extracts, through high-performance liquid chromatography (HPLC-DAD), as well as, evaluation of acute toxicity, hypolipidemic activity of the extract (administered orally: 100 and 200 mg/kg), in induced hyperlipidemic mice, and investigation of the possible mechanism of action, through molecular dynamics simulations. The physicochemical assays were within the pharmacopoeial standards. The presence of the vitexin, orientin and isoorientin was confirmed by HPLC-DAD. No clinical signs of toxicity were observed in the evaluated animals. The hypolipidemic analysis presented a reduction in total cholesterol levels (TC) and an increase in high-density lipoprotein (HDL-c), statistically significant (p < 0.05), when compared to control only induced, while triglycerides indices (TG) did not show a significant response. The analysis of molecular docking showed that the compounds in general showed stability in practically the entire simulation, mainly the vitexin, when associated with the LCAT enzyme, demonstrating that the evaluated extract has lipid-lowering activity, possibly due to the presence of the identified bioactive compounds. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-03-31 |
dc.date.accessioned.fl_str_mv |
2023-08-08T15:31:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
LEAL, Ana Edil?ia Barbosa Pereira. Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae). 2022. 104f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana,Feira de Santana, 2022. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/1502 |
identifier_str_mv |
LEAL, Ana Edil?ia Barbosa Pereira. Controle de qualidade da droga vegetal e avalia??o da atividade hipolipid?mica in vivo do extrato aquoso dos frutos de passiflora Cincinnata mast. (Passifloraceae). 2022. 104f. Tese (Doutorado Acad?mico em Biotecnologia) - Universidade Estadual de Feira de Santana,Feira de Santana, 2022. |
url |
http://tede2.uefs.br:8080/handle/tede/1502 |
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por |
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por |
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openAccess |
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Universidade Estadual de Feira de Santana |
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