Diversidade microbiana em infecções endodônticas primárias associadas ao traumatismo dentário

• Ano de defesa: 2014
• Autor(a) principal: Cunha, Fernando Marques da
• Orientador(a): Sassone, Luciana Moura
• Banca de defesa: Fidel, Rivail Antonio Sergio , Carvalho, Marco Antonio Alencar de , Bruno, Adilia Maria Vieira , Amaral, Georgiana , Vilhena, Fernando Sili
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Rio de Janeiro
• Programa de Pós-Graduação: Programa de Pós-Graduação em Odontologia
• Departamento: Centro Biomédico::Faculdade de Odontologia
• País: BR
• Palavras-chave em Português: Bactérias; Biologia Molecular; Periodontite apical; Sondas de DNA; Traumatologia
• Palavras-chave em Inglês: Bacteria; Molecular biology; Apical periodontitis; DNA probes; Traumatology
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::ENDODONTIA
• Link de acesso: http://www.bdtd.uerj.br/handle/1/14057

This study aims to investigate the microbiota of root canals, seeking to identify and quantify these microorganisms. Thirty one teeth with related primary infection due to dental trauma were selected. Microbiological samples were collected from the root canal using Hedströen files and sterile absorbent paper points. The checkerboard DNA-DNA hybridization molecular technique was used to detect up to 38 bacterial species in each sample using specific DNA probes. Microbiological data were expressed as mean percentage (prevalence), mean level (score) and the proportion of each species in each sample. The independent and Pearson correlation tests were used to correlate the bacterias tested with the types of trauma (p &#8804; 0.05). An average of 13.74 species per sample was found. The most prevalent species were P. melaninogenica (84%), E. faecalis (77%), C. gracilis (71%) and F. nucleatum sp. vicentii (71%). The species that showed low prevalence were A. odontolyticus (26%), P. acnes (26%), E. corrodens (23%), A. israelii (16%), A. gerencseriae (16%), P. endodontalis (16%) and A. naeslundii (13%). The species F. nucleatum sp. vicentii, P. nigrescens, T. denticola, C. gingivalis, C. rectus and P. gingivalis showed significantly higher mean levels between cases of trauma to the supporting tissues and cases of dental trauma (P <0.05). T. denticola, P. gingivalis and C. gingivalis also showed significant higher ratios in the trauma to the supporting tissues cases (p> 0.05). Based on the obtained results, it can be concluded that the bacterial profile of pulpal necrosis caused by traumactic injuries may vary depending on the occurrence of damage to the supporting tissues.