Comparação do perfil proteico de espermatozoides em diferentes espécies

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Silvino, Mariana Baraldi Silva
Orientador(a): Moura, Arlindo de Alencar Araripe Noronha
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Capacitação espermática
Fertilização
Proteínas espermáticas
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/44200
Resumo: Proteins present in seminal plasma of domestic animals present several functions in sperm physiology, acting in capacitation processes, acrosome reaction, interaction between gametes and sperm protection. Thus, the present study characterized proteins present in sperm cells from sheep, goats, horses, pigs and dogs. By means of mass spectrometry, a total of 128 proteins were identified in the enriched fraction of spermatozoa of the species used. From this total, identified proteins were found in all species, however six proteins were present in four of the five species studied: angiotensin-converting enzyme, ATP synthase subunit, hexokinase 1, malate dehydrogenase, pyruvate dehydrogenase, succinyl- CoA and a total of 14 proteins were identified in at least 3 species. This study was the first to characterize the proteins present in the enriched fraction of spermatozoon of dogs, in addition to comparing five species. Despite the high similarity between some species, the spermatozoid presents protein differences in its composition, which could explain the different variations in the fertilization process.
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spelling Silvino, Mariana Baraldi SilvaVasconcelos, Fábio RogerMoura, Arlindo de Alencar Araripe Noronha2019-07-29T16:14:25Z2019-07-29T16:14:25Z2018SILVINO, Mariana Baraldi Silva. Comparação do perfil proteico de espermatozoides em diferentes espécies. 2019. 108 f. Tese (Doutorado em Zootecnia) – Universidade Federal do Ceará, Fortaleza, 2018.http://www.repositorio.ufc.br/handle/riufc/44200Proteins present in seminal plasma of domestic animals present several functions in sperm physiology, acting in capacitation processes, acrosome reaction, interaction between gametes and sperm protection. Thus, the present study characterized proteins present in sperm cells from sheep, goats, horses, pigs and dogs. By means of mass spectrometry, a total of 128 proteins were identified in the enriched fraction of spermatozoa of the species used. From this total, identified proteins were found in all species, however six proteins were present in four of the five species studied: angiotensin-converting enzyme, ATP synthase subunit, hexokinase 1, malate dehydrogenase, pyruvate dehydrogenase, succinyl- CoA and a total of 14 proteins were identified in at least 3 species. This study was the first to characterize the proteins present in the enriched fraction of spermatozoon of dogs, in addition to comparing five species. Despite the high similarity between some species, the spermatozoid presents protein differences in its composition, which could explain the different variations in the fertilization process.Proteínas presentes no plasma seminal de animais domésticos apresentam diversas funções na fisiologia espermática, atuando em processos de capacitação, reação acrossômica, interação entre gametas e proteção ao espermatozoide. Desse modo, o presente estudo caracterizou proteínas presentes em células espermáticas de ovinos, caprinos, equinos, suínos e cães. Por meio de espectrometria de massas, foram identificadas um total de 128 proteínas no espermatozoide das espécies utilizadas. Seis proteínas estavam presentes em quatro das cinco espécies estudadas, sendo elas a angiotensin-converting enzyme, ATP-synthase subunit α, hexoquinase 1, malate dehydrogenase, pyruvate dehydrogenase e succinyl-CoA. Foi feita ontologia gênica, interação interproteica e filogenia dessas seis proteínas. Foi possível identificar 10 proteínas comuns às espécies ruminantes. Esse estudo foi o primeiro a caracterizar as proteínas presentes em de espermatozoides totais de cão, além de comparar o perfil proteico das cinco espécies. Apesar da alta similaridade entre algumas espécies, o espermatozoide apresenta diferenças proteicas na sua composição, o que poderia explicar as diferentes variações no processo de fertilização.Capacitação espermáticaFertilizaçãoProteínas espermáticasComparação do perfil proteico de espermatozoides em diferentes espéciesComparison of protein profile of sperm in different speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/44200/6/license.txt8a4605be74aa9ea9d79846c1fba20a33MD56ORIGINAL2018_tese_mbsilva.pdf2018_tese_mbsilva.pdfapplication/pdf2306177http://repositorio.ufc.br/bitstream/riufc/44200/5/2018_tese_mbsilva.pdfda38045076105ac80ab6824d4809ce3bMD55riufc/442002020-05-20 09:09:17.29oai:repositorio.ufc.br:riufc/44200Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2020-05-20T12:09:17Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.pt_BR.fl_str_mv Comparação do perfil proteico de espermatozoides em diferentes espécies
dc.title.en.pt_BR.fl_str_mv Comparison of protein profile of sperm in different species
title Comparação do perfil proteico de espermatozoides em diferentes espécies
spellingShingle Comparação do perfil proteico de espermatozoides em diferentes espécies
Silvino, Mariana Baraldi Silva
Capacitação espermática
Fertilização
Proteínas espermáticas
title_short Comparação do perfil proteico de espermatozoides em diferentes espécies
title_full Comparação do perfil proteico de espermatozoides em diferentes espécies
title_fullStr Comparação do perfil proteico de espermatozoides em diferentes espécies
title_full_unstemmed Comparação do perfil proteico de espermatozoides em diferentes espécies
title_sort Comparação do perfil proteico de espermatozoides em diferentes espécies
author Silvino, Mariana Baraldi Silva
author_facet Silvino, Mariana Baraldi Silva
author_role author
dc.contributor.co-advisor.none.fl_str_mv Vasconcelos, Fábio Roger
dc.contributor.author.fl_str_mv Silvino, Mariana Baraldi Silva
dc.contributor.advisor1.fl_str_mv Moura, Arlindo de Alencar Araripe Noronha
contributor_str_mv Moura, Arlindo de Alencar Araripe Noronha
dc.subject.por.fl_str_mv Capacitação espermática
Fertilização
Proteínas espermáticas
topic Capacitação espermática
Fertilização
Proteínas espermáticas
description Proteins present in seminal plasma of domestic animals present several functions in sperm physiology, acting in capacitation processes, acrosome reaction, interaction between gametes and sperm protection. Thus, the present study characterized proteins present in sperm cells from sheep, goats, horses, pigs and dogs. By means of mass spectrometry, a total of 128 proteins were identified in the enriched fraction of spermatozoa of the species used. From this total, identified proteins were found in all species, however six proteins were present in four of the five species studied: angiotensin-converting enzyme, ATP synthase subunit, hexokinase 1, malate dehydrogenase, pyruvate dehydrogenase, succinyl- CoA and a total of 14 proteins were identified in at least 3 species. This study was the first to characterize the proteins present in the enriched fraction of spermatozoon of dogs, in addition to comparing five species. Despite the high similarity between some species, the spermatozoid presents protein differences in its composition, which could explain the different variations in the fertilization process.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-07-29T16:14:25Z
dc.date.available.fl_str_mv 2019-07-29T16:14:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVINO, Mariana Baraldi Silva. Comparação do perfil proteico de espermatozoides em diferentes espécies. 2019. 108 f. Tese (Doutorado em Zootecnia) – Universidade Federal do Ceará, Fortaleza, 2018.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/44200
identifier_str_mv SILVINO, Mariana Baraldi Silva. Comparação do perfil proteico de espermatozoides em diferentes espécies. 2019. 108 f. Tese (Doutorado em Zootecnia) – Universidade Federal do Ceará, Fortaleza, 2018.
url http://www.repositorio.ufc.br/handle/riufc/44200
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instname:Universidade Federal do Ceará (UFC)
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instname_str Universidade Federal do Ceará (UFC)
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bitstream.url.fl_str_mv http://repositorio.ufc.br/bitstream/riufc/44200/6/license.txt
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