Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo

Detalhes bibliográficos
Ano de defesa: 2016
Autor(a) principal: Silva, Alison Batista da
Orientador(a): Pessoa, Otília Deusdênia Loiola
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Bactéria marinha
Salinispora arenicola
Streptomyces sp
Rifamicina
Link de acesso: http://www.repositorio.ufc.br/handle/riufc/53733
Resumo: The present work describes the chemical investigation of two active bacteria strains identified as Salinispora arenicola (BRA-213) and Streptomyces sp. (BRA-199), both isolated from sediments of Saint Peter and Saint Paul’s Archipelago – State of Pernambuco. From S. arenicola were isolated six compounds: 3-hydroxy-6-methoxy-N-methylindolin-2-one, 6-methoxy-N-methyl-isatin, N-acetyl-β-oxo-tryptamine, saliniketal A, 4-(2’-oxopropyl)-3-oxorifamycin S and 27-demethoxy-27-hydroxy-4-(2’-oxopropyl)-3-oxorifamycin S. In addition, three staurosporines (staurosporine, N-methyl-staurosporine, and hydroxy-N-methyl-staurosporine) were identified by LC-MS and research in the AntiMarin database which are responsible for the cytotoxic activity of S. arenicola extract (IC50 0.55 µg/mL). Among the isolated compounds the rifamycins are been reported for the first time and the compounds 3-hydroxy-6-methoxy-N-methyl indolin-2-one and 6-methoxy-N-methyl-isatin are recorded first from microorganisms. From Streptomyces sp. were isolated the compounds piericidin A, cyclo-hydroxy-Pro-Leu, cyclo-Trp-Pro, cyclo-Phe-Pro while compound glicopiericidin, also was identified by LC-MS and AntiMarin database. The structures of the isolated compounds were determined by the interpretation of its 1H and 13C NMR (1D and 2D) and mass spectrometry. Piericidin A showed cytotoxic activity justifying the activity exhibited by the extract of Streptomyces sp. (IC50 0.07 µg/mL).
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spelling Silva, Alison Batista daTorres, Maria da Conceição MenezesPessoa, Otília Deusdênia Loiola2020-08-31T11:26:45Z2020-08-31T11:26:45Z2016SILVA, Alison Batista da. Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo. 2016. 115 f. Dissertação (Mestrado em Química) - Universidade Federal do Ceará, Fortaleza, 2016.http://www.repositorio.ufc.br/handle/riufc/53733The present work describes the chemical investigation of two active bacteria strains identified as Salinispora arenicola (BRA-213) and Streptomyces sp. (BRA-199), both isolated from sediments of Saint Peter and Saint Paul’s Archipelago – State of Pernambuco. From S. arenicola were isolated six compounds: 3-hydroxy-6-methoxy-N-methylindolin-2-one, 6-methoxy-N-methyl-isatin, N-acetyl-β-oxo-tryptamine, saliniketal A, 4-(2’-oxopropyl)-3-oxorifamycin S and 27-demethoxy-27-hydroxy-4-(2’-oxopropyl)-3-oxorifamycin S. In addition, three staurosporines (staurosporine, N-methyl-staurosporine, and hydroxy-N-methyl-staurosporine) were identified by LC-MS and research in the AntiMarin database which are responsible for the cytotoxic activity of S. arenicola extract (IC50 0.55 µg/mL). Among the isolated compounds the rifamycins are been reported for the first time and the compounds 3-hydroxy-6-methoxy-N-methyl indolin-2-one and 6-methoxy-N-methyl-isatin are recorded first from microorganisms. From Streptomyces sp. were isolated the compounds piericidin A, cyclo-hydroxy-Pro-Leu, cyclo-Trp-Pro, cyclo-Phe-Pro while compound glicopiericidin, also was identified by LC-MS and AntiMarin database. The structures of the isolated compounds were determined by the interpretation of its 1H and 13C NMR (1D and 2D) and mass spectrometry. Piericidin A showed cytotoxic activity justifying the activity exhibited by the extract of Streptomyces sp. (IC50 0.07 µg/mL).CAPESNo presente trabalho descreve-se a investigação química de duas cepas bacterianas ativas identificadas como Salinispora arenicola (BRA-213) e Streptomyces sp. (BRA-199), ambas isoladas de sedimentos coletados no arquipélago de São Pedro e São Paulo – Estado de Pernambuco. De S. arenicola foram isolados seis compostos: 3-hidroxi-6-metoxi-N-metilindolin-2-ona, 6-metoxi-N-metil-isatina, N-acetil-β-oxo-triptamina, salinicetal A, 4-hidroxi-4-(2’-oxopropil)-3-oxo rifamicina S e 27-desmetil-4-hidroxi-4-(2’-oxopropil)-3-oxo rifamicina S. Adicionalmente, foram identificados por LC-MS e pesquisa no banco de dados AntiMarin três estaurosporinas (estaurosporina, N-metil-estaurosporina e hidroxi-N-metil-estaurosporina), as quais são responsáveis pela atividade citotóxica do extrato de S. arenicola (CI50 0,55 µg/mL). Entre os compostos isolados, as rifamicinas estão sendo relatadas pela primeira vez e os compostos 3-hidroxi-6-metoxi-N-metilindolin-2-ona, 6-metoxi-N-metil-isatina estão sendo registrados pela primeira vez a partir de microrganismos. De Streptomyces sp. foram isolados os compostos piericidina A, ciclo-hidroxi-Pro-Leu, ciclo-Trp-Pro, ciclo-Phe-Pro, enquanto o composto glicopiericidina A, foi identificado por LC-MS e pesquisa no AntiMarin. As estruturas dos compostos isolados foram determinadas através da interpretação de seus espectros de RMN 1H e 13C (1D e 2D) e espectrometria de massas. A piericidina A apresentou atividade citotóxica justificando, em parte, a atividade exibida pelo extrato (CI50 0,07 µg/mL) de Streptomyces sp.Bactéria marinhaSalinispora arenicolaStreptomyces spRifamicinaInvestigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São PauloChemical investigation of extract from marine bacteria associated with the sediment of St. Peter and St. Paul archipelagoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccessORIGINAL2016_dis_absilva.pdf2016_dis_absilva.pdfapplication/pdf5743414http://repositorio.ufc.br/bitstream/riufc/53733/5/2016_dis_absilva.pdf8eb4b966d19cd934d7a2dda1cd8bafe3MD55LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufc.br/bitstream/riufc/53733/6/license.txt8a4605be74aa9ea9d79846c1fba20a33MD56riufc/537332020-08-31 08:37:50.379oai:repositorio.ufc.br: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Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2020-08-31T11:37:50Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.pt_BR.fl_str_mv Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
dc.title.en.pt_BR.fl_str_mv Chemical investigation of extract from marine bacteria associated with the sediment of St. Peter and St. Paul archipelago
title Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
spellingShingle Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
Silva, Alison Batista da
Bactéria marinha
Salinispora arenicola
Streptomyces sp
Rifamicina
title_short Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
title_full Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
title_fullStr Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
title_full_unstemmed Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
title_sort Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo
author Silva, Alison Batista da
author_facet Silva, Alison Batista da
author_role author
dc.contributor.co-advisor.none.fl_str_mv Torres, Maria da Conceição Menezes
dc.contributor.author.fl_str_mv Silva, Alison Batista da
dc.contributor.advisor1.fl_str_mv Pessoa, Otília Deusdênia Loiola
contributor_str_mv Pessoa, Otília Deusdênia Loiola
dc.subject.por.fl_str_mv Bactéria marinha
Salinispora arenicola
Streptomyces sp
Rifamicina
topic Bactéria marinha
Salinispora arenicola
Streptomyces sp
Rifamicina
description The present work describes the chemical investigation of two active bacteria strains identified as Salinispora arenicola (BRA-213) and Streptomyces sp. (BRA-199), both isolated from sediments of Saint Peter and Saint Paul’s Archipelago – State of Pernambuco. From S. arenicola were isolated six compounds: 3-hydroxy-6-methoxy-N-methylindolin-2-one, 6-methoxy-N-methyl-isatin, N-acetyl-β-oxo-tryptamine, saliniketal A, 4-(2’-oxopropyl)-3-oxorifamycin S and 27-demethoxy-27-hydroxy-4-(2’-oxopropyl)-3-oxorifamycin S. In addition, three staurosporines (staurosporine, N-methyl-staurosporine, and hydroxy-N-methyl-staurosporine) were identified by LC-MS and research in the AntiMarin database which are responsible for the cytotoxic activity of S. arenicola extract (IC50 0.55 µg/mL). Among the isolated compounds the rifamycins are been reported for the first time and the compounds 3-hydroxy-6-methoxy-N-methyl indolin-2-one and 6-methoxy-N-methyl-isatin are recorded first from microorganisms. From Streptomyces sp. were isolated the compounds piericidin A, cyclo-hydroxy-Pro-Leu, cyclo-Trp-Pro, cyclo-Phe-Pro while compound glicopiericidin, also was identified by LC-MS and AntiMarin database. The structures of the isolated compounds were determined by the interpretation of its 1H and 13C NMR (1D and 2D) and mass spectrometry. Piericidin A showed cytotoxic activity justifying the activity exhibited by the extract of Streptomyces sp. (IC50 0.07 µg/mL).
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2020-08-31T11:26:45Z
dc.date.available.fl_str_mv 2020-08-31T11:26:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv SILVA, Alison Batista da. Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo. 2016. 115 f. Dissertação (Mestrado em Química) - Universidade Federal do Ceará, Fortaleza, 2016.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufc.br/handle/riufc/53733
identifier_str_mv SILVA, Alison Batista da. Investigação química de extratos de bactérias marinhas associadas ao sedimento do arquipélago de São Pedro e São Paulo. 2016. 115 f. Dissertação (Mestrado em Química) - Universidade Federal do Ceará, Fortaleza, 2016.
url http://www.repositorio.ufc.br/handle/riufc/53733
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reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
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