Modelo murino de inflamação intestinal crônica: Avaliação quantitativa da dinâmica da microbiota intestinal

• Ano de defesa: 2007
• Autor(a) principal: Bisso, Mônica Lourenço
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Programa de Pós-graduação em Patologia; Patologia
• Palavras-chave em Português: Microbiota; Inflamação intestinal; Quimioterápico; Amendoim; Trato gastrointestinal; Intestino delgado; Microbiologia; Resistência microbiana a drogas; Inflammatory diseases; Peanuts; Quimioterápic; CNPQ::CIENCIAS DA SAUDE::MEDICINA
• Link de acesso: https://app.uff.br/riuff/handle/1/17877

Aim: Evaluate and characterize the possible differences in the gut microbiota of normal and immune mice submitted to an antigen specific gut inflammation, and its response in the GALT, as well as the maintenance or disruption of homeostasis. Methods: Eight-week-old male C57BL/6 mice were divided in groups 1 and 2, where group 2 received chemotherapy in the drinking water during the challenge diet period (4 weeks). Groups 1 and 2 were subdivided in four subgroups (CR, IR, CA e IA): IA was inoculated with peanut protein extract (immunize group), and received peanut during the challenge diet; group IR was inoculated with peanut protein extract, but continuous feeding on commercial chow; Group CR was sham immunized with saline (control group) and was only fed on commercial chow; and finally group CA was sham immunized with saline, and received peanut during the challenge diet period. One pellet of stool sample was collected from each mouse, weekly during of challenge diet for quantitative identification of colony-forming units (CFUs) of aerobic microorganisms (enterococos, coliformes and mesophiles) of the microbiota. After 28 days, all animals were sacrificed, to determine the extent of macro and microscopic inflammatory process of the gut. Results: We observed that as of the third week of chemotherapy the CFUs present significant reduction compared to control groups and a significant modification of the gut microbiota kinetics was also observed. Animals of both IA groups presented significant alterations of the histological architecture with more severe alterations in the group that drank chemotherapy. Conclusion: in our model the use of chemotherapy did not ameliorate gut alterations or clinical signs in animals submitted to an antigen specific chronic inflammation of the gut.