Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro

Detalhes bibliográficos
Ano de defesa: 2021
Autor(a) principal: Rosa, Gabriela Danelli lattes
Orientador(a): Bailão, Alexandre Melo lattes
Banca de defesa: Bailão, Alexandre Melo, Lima, Patricia de Sousa, Rocha, Thiago Lopes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Genética e Biologia Molecular (ICB)
Departamento: Instituto de Ciências Biológicas - ICB (RG)
País: Brasil
Palavras-chave em Português:
Proteômica
Cromoblastomicose
CBM
Fonsecaea pedrosoi
Temperatura do hospedeiro
Palavras-chave em Inglês:
Proteomics
Chromoblastomycosis
Host temperature
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/12133
Resumo: Chromoblastomycosis (CBM) is a chronic subcutaneous mycosis, very common in tropical and subtropical regions and affects many men related to rural activities. Lesions of this disease can appear in five clinical forms and treatments are difficult due to the recalcitrant nature of the disease. The main causative agent of CBM is the fungus Fonsecaea pedrosoi, which is a polymorphic, melanized fungus that presents a certain phenotypic plasticity in face of temperature variations. Thermotolerance is one of the virulence factors of virulence for this pathogen, since it survives the temperature increase, like that of the host. However, no work so far has searched to characterize the adapted cellular processes. Proteins are part of the strategies used in response to a variety of stressors, such as host temperature. In this work we mapped the intracellular proteomic profile of F.pedrosoi cultivated under different temperature conditions (28 and 37 °C) for 24 hours. It was possible to identify by means of Liquid Chromatography coupled to Mass Spectrometry (Nano UPLC-MSᴱ) a total of 486 differentially expressed proteins, 101 of which were up-regulated and 385 down-regulated. It was possible to observe that F. pedrosoi seems to respond to temperature stress by repressing central carbon metabolism pathways, such as glycolysis, tricarboxylic acid cycle (TCA) and glyoxylate cycle, cell wall biosynthesis and melanin and induced amino acid degradation enzymes and antioxidant proteins.
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spelling Bailão, Alexandre Melohttp://lattes.cnpq.br/5415221996976886Bailão, Alexandre MeloLima, Patricia de SousaRocha, Thiago Lopeshttp://lattes.cnpq.br/3632033730008798Rosa, Gabriela Danelli2022-06-27T15:28:31Z2022-06-27T15:28:31Z2021-09-29Rosa, G. D. Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro. 2022. 79 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2021.http://repositorio.bc.ufg.br/tede/handle/tede/12133Chromoblastomycosis (CBM) is a chronic subcutaneous mycosis, very common in tropical and subtropical regions and affects many men related to rural activities. Lesions of this disease can appear in five clinical forms and treatments are difficult due to the recalcitrant nature of the disease. The main causative agent of CBM is the fungus Fonsecaea pedrosoi, which is a polymorphic, melanized fungus that presents a certain phenotypic plasticity in face of temperature variations. Thermotolerance is one of the virulence factors of virulence for this pathogen, since it survives the temperature increase, like that of the host. However, no work so far has searched to characterize the adapted cellular processes. Proteins are part of the strategies used in response to a variety of stressors, such as host temperature. In this work we mapped the intracellular proteomic profile of F.pedrosoi cultivated under different temperature conditions (28 and 37 °C) for 24 hours. It was possible to identify by means of Liquid Chromatography coupled to Mass Spectrometry (Nano UPLC-MSᴱ) a total of 486 differentially expressed proteins, 101 of which were up-regulated and 385 down-regulated. It was possible to observe that F. pedrosoi seems to respond to temperature stress by repressing central carbon metabolism pathways, such as glycolysis, tricarboxylic acid cycle (TCA) and glyoxylate cycle, cell wall biosynthesis and melanin and induced amino acid degradation enzymes and antioxidant proteins.A Cromoblastomicose (CBM) é uma micose subcutânea crônica, muito comum em regiões tropicais e subtropicais e acomete em maioria homens relacionados a atividades rurais. As lesões dessa doença podem aparecer em cinco formas clínicas e os tratamentos são dificultados devido à natureza recalcitrante da doença. O principal agente causador da CBM é o fungo Fonsecaea pedrosoi, o qual é um fungo polimórfico, melanizado e que apresenta certa plasticidade fenotípica frente a variações de temperatura. A termotolerância é um dos fatores de virulência descritos para este patógeno, uma vez que este sobrevive ao aumento de temperatura, como a do hospedeiro. Porém, nenhum trabalho até o momento buscou caracterizar os processos celulares envolvidos nessa adaptação. As proteínas fazem parte das estratégias utilizadas em resposta a uma variedade de estressores, tais como a temperatura do hospedeiro. Neste trabalho nós mapeamos o perfil proteômico intracelular de F.pedrosoi cultivado em diferentes condições de temperatura (28 e 37°C) por 24 horas. Foi possível identificar por meio de Cromatografia Líquida acoplada a Espectrometria de Massa (Nano UPLC-MSᴱ) um total de 486 proteínas diferencialmente expressas, sendo que, 101 foram reguladas positivamente e 385 reguladas negativamente. Foi possível observar que F. pedrosoi parece responder ao estresse de temperatura reprimindo vias do metabolismo central de carbono, como a glicólise, ciclo do ácido tricarboxílico (TCA) e ciclo do glioxilato, biossíntese de parede celular e melanina e induziu enzimas de degradação de aminoácidos e proteínas antioxidantes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia Molecular (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalinfo:eu-repo/semantics/openAccessProteômicaCromoblastomicoseCBMFonsecaea pedrosoiTemperatura do hospedeiroProteomicsChromoblastomycosisHost temperatureCIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOSAnálise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiroProteomic analysis of the human pathogenic fungus Fonsecaea pedrosoi subjected to host temperatureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis58500500500500236491reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/2ada3ae6-c5c6-4e35-8809-fba70fdbe938/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/37fa2162-dbe8-44d1-83b6-6162810c16b4/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Gabriela Danelli Rosa - 2022.pdfDissertação - Gabriela Danelli Rosa - 2022.pdfapplication/pdf3689127http://repositorio.bc.ufg.br/tede/bitstreams/6bc392e6-b0ee-4756-b856-fe7057ee972d/downloaddc85c1c65811d3ddb5edecaf97e5ed7dMD53tede/121332022-06-27 12:28:31.678http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/12133http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttps://repositorio.bc.ufg.br/tedeserver/oai/requestgrt.bc@ufg.bropendoar:oai:repositorio.bc.ufg.br:tede/12342022-06-27T15:28:31Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)falseTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=
dc.title.pt_BR.fl_str_mv Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
dc.title.alternative.eng.fl_str_mv Proteomic analysis of the human pathogenic fungus Fonsecaea pedrosoi subjected to host temperature
title Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
spellingShingle Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
Rosa, Gabriela Danelli
Proteômica
Cromoblastomicose
CBM
Fonsecaea pedrosoi
Temperatura do hospedeiro
Proteomics
Chromoblastomycosis
Host temperature
CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
title_short Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
title_full Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
title_fullStr Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
title_full_unstemmed Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
title_sort Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro
author Rosa, Gabriela Danelli
author_facet Rosa, Gabriela Danelli
author_role author
dc.contributor.advisor1.fl_str_mv Bailão, Alexandre Melo
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5415221996976886
dc.contributor.referee1.fl_str_mv Bailão, Alexandre Melo
dc.contributor.referee2.fl_str_mv Lima, Patricia de Sousa
dc.contributor.referee3.fl_str_mv Rocha, Thiago Lopes
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3632033730008798
dc.contributor.author.fl_str_mv Rosa, Gabriela Danelli
contributor_str_mv Bailão, Alexandre Melo
Bailão, Alexandre Melo
Lima, Patricia de Sousa
Rocha, Thiago Lopes
dc.subject.por.fl_str_mv Proteômica
Cromoblastomicose
CBM
Fonsecaea pedrosoi
Temperatura do hospedeiro
topic Proteômica
Cromoblastomicose
CBM
Fonsecaea pedrosoi
Temperatura do hospedeiro
Proteomics
Chromoblastomycosis
Host temperature
CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
dc.subject.eng.fl_str_mv Proteomics
Chromoblastomycosis
Host temperature
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
description Chromoblastomycosis (CBM) is a chronic subcutaneous mycosis, very common in tropical and subtropical regions and affects many men related to rural activities. Lesions of this disease can appear in five clinical forms and treatments are difficult due to the recalcitrant nature of the disease. The main causative agent of CBM is the fungus Fonsecaea pedrosoi, which is a polymorphic, melanized fungus that presents a certain phenotypic plasticity in face of temperature variations. Thermotolerance is one of the virulence factors of virulence for this pathogen, since it survives the temperature increase, like that of the host. However, no work so far has searched to characterize the adapted cellular processes. Proteins are part of the strategies used in response to a variety of stressors, such as host temperature. In this work we mapped the intracellular proteomic profile of F.pedrosoi cultivated under different temperature conditions (28 and 37 °C) for 24 hours. It was possible to identify by means of Liquid Chromatography coupled to Mass Spectrometry (Nano UPLC-MSᴱ) a total of 486 differentially expressed proteins, 101 of which were up-regulated and 385 down-regulated. It was possible to observe that F. pedrosoi seems to respond to temperature stress by repressing central carbon metabolism pathways, such as glycolysis, tricarboxylic acid cycle (TCA) and glyoxylate cycle, cell wall biosynthesis and melanin and induced amino acid degradation enzymes and antioxidant proteins.
publishDate 2021
dc.date.issued.fl_str_mv 2021-09-29
dc.date.accessioned.fl_str_mv 2022-06-27T15:28:31Z
dc.date.available.fl_str_mv 2022-06-27T15:28:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv Rosa, G. D. Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro. 2022. 79 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2021.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/12133
identifier_str_mv Rosa, G. D. Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi submetido à temperatura do hospedeiro. 2022. 79 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2021.
url http://repositorio.bc.ufg.br/tede/handle/tede/12133
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language por
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dc.relation.confidence.fl_str_mv 500
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dc.relation.cnpq.fl_str_mv 649
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Genética e Biologia Molecular (ICB)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Ciências Biológicas - ICB (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
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