Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C
Ano de defesa: | 2017 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Goiás
|
Programa de Pós-Graduação: |
Programa de Pós-graduação em Genética e Biologia Molecular (ICB)
|
Departamento: |
Instituto de Ciências Biológicas - ICB (RG)
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.bc.ufg.br/tede/handle/tede/10579 |
Resumo: | The Polymorphic fungus Fonsecaea pedrosoi is the main etiological agent of Chromoblastomycosis (CBM), characterized as a chronic cutaneous mycotic infection, occurring mainly in tropical regions. The limited availability of effective therapeutic protocols coupled with CBM clinical polymorphism lead to often prolonged and high relapse rate therapies. Results regarding the molecular biology of F. pedrosoi are scarce however, some virulence factors have already been described, evidencing the need to explore the mechanisms used by this pathogen during infection. The present work carried out in silico analyzes under specific culture conditions, with temperatures of 22º C and 36º C, aiming to better understand the proteome of this fungus, both intracellular and secreted. In order to identify these proteins, we used bioinformatic tools that are available online and allowed the prediction of protein sets that can be secreted by classical and non-classical routes. After the in silico prediction method, proteins were identified in the fungal extracts by the Ultraperformance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MSE) methodology. Those identified in the extracts that brought a more reliable interpretation corroborated the results in silico. The proteins identified stand out for being related to various biological processes, such as metabolism of carbon compounds, energy, proteins related to thermal and oxidative stress, defense, proteins related to the cell cycle, among other functions. The analyzes showed that the vast majority of identified proteins are secreted by alternative pathways, and the results point to the great importance of the biological roles that these secreted proteins can play during the onset of infection. Thus, we observed that the fungus presents variations in protein expression levels according to the condition addressed and may reflect on its behavior and responses to the successful establishment of the infection. |
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Bailão, Alexandre Melohttp://lattes.cnpq.br/5415221996976886Bailão, lexandre MeloPaccez, Juliano DomiraciNovaes, Evandrohttp://lattes.cnpq.br/8201464584315371Lima, Davi Vinícius de2020-09-08T12:07:29Z2020-09-08T12:07:29Z2017-09-11LIMA, D. V. Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C. 2017. 51 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/10579The Polymorphic fungus Fonsecaea pedrosoi is the main etiological agent of Chromoblastomycosis (CBM), characterized as a chronic cutaneous mycotic infection, occurring mainly in tropical regions. The limited availability of effective therapeutic protocols coupled with CBM clinical polymorphism lead to often prolonged and high relapse rate therapies. Results regarding the molecular biology of F. pedrosoi are scarce however, some virulence factors have already been described, evidencing the need to explore the mechanisms used by this pathogen during infection. The present work carried out in silico analyzes under specific culture conditions, with temperatures of 22º C and 36º C, aiming to better understand the proteome of this fungus, both intracellular and secreted. In order to identify these proteins, we used bioinformatic tools that are available online and allowed the prediction of protein sets that can be secreted by classical and non-classical routes. After the in silico prediction method, proteins were identified in the fungal extracts by the Ultraperformance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MSE) methodology. Those identified in the extracts that brought a more reliable interpretation corroborated the results in silico. The proteins identified stand out for being related to various biological processes, such as metabolism of carbon compounds, energy, proteins related to thermal and oxidative stress, defense, proteins related to the cell cycle, among other functions. The analyzes showed that the vast majority of identified proteins are secreted by alternative pathways, and the results point to the great importance of the biological roles that these secreted proteins can play during the onset of infection. Thus, we observed that the fungus presents variations in protein expression levels according to the condition addressed and may reflect on its behavior and responses to the successful establishment of the infection.O fungo polimórfico Fonsecaea pedrosoi é o principal agente etiológico da Cromoblastomicose (CBM), caracterizada como uma infecção micótica cutânea crônica, ocorrendo principalmente em regiões tropicais. A limitada disponibilidade de protocolos terapêuticos eficazes somada ao polimorfismo clínico da CBM, conduzem a terapias que na maioria das vezes são prolongadas e de elevada taxa recidiva. Resultados a respeito da biologia molecular de F. pedrosoi são escassos, no entanto, alguns fatores de virulência já foram descritos, evidenciando a necessidade de se explorar os mecanismos utilizados por este patógeno durante a infecção. O presente trabalho realizou análises in silico e em condições de cultivo específicas, com temperaturas de 22º C e 36º C, visando entender melhor o proteoma deste fungo, tanto intracelular, quanto o secretado. Afim de identificar essas proteínas, foram utilizadas ferramentas bioinformáticas que se encontram disponíveis on-line e permitiram realizar a predição de conjuntos de proteínas que podem ser secretadas por rotas clássicas e não clássicas. Após o método de predição in silico, realizou-se a identificação de proteinas nos extratos fúngicos por meio da metodologia de Cromatografia Líquida de Ultraperformance acoplada à Espectrometria de Massas (UPLC-MSE). Os resultados in silico foram corroborados pelos identificados nos extratos o que trouxe uma interpretação mais fidedigna. Dentre as proteínas identificadas podemos destacar que estão relacionadas à diversos processos biológicos, tais como, metabolismo de compostos de carbono, energia, proteínas relacionadas ao estresse térmico e oxidativo, defesa, proteínas relacionadas ao ciclo celular, entre outras funções. As análises demostraram que a grande maioria das proteínas identificadas são secretadas por vias alternativas, e os resultados apontam para a grande importância dos papeis biológicos que essas proteínas secretadas podem desempenhar durante o estabelecimento da infecção. Dessa forma, observa-se que o fungo apresenta variações nos níveis de expressão proteica de acordo com a condição abordada podendo refletir em seu comportamento e nas respostas para o sucesso do estabelecimento da infecção.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2020-09-05T14:10:28Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Davi Vinícius de Lima - 2017.pdf: 1647183 bytes, checksum: 0edd6ec967010bf7bef4a485cc98fbe6 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-08T12:07:29Z (GMT) No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Davi Vinícius de Lima - 2017.pdf: 1647183 bytes, checksum: 0edd6ec967010bf7bef4a485cc98fbe6 (MD5)Made available in DSpace on 2020-09-08T12:07:29Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Davi Vinícius de Lima - 2017.pdf: 1647183 bytes, checksum: 0edd6ec967010bf7bef4a485cc98fbe6 (MD5) Previous issue date: 2017-09-11Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia Molecular (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCromoblastomicoseProteomaSecretomaVirulênciaChromoblastomycosisProteomeVirulenceCIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOSAnálise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º CProteomic analysis of the human pathogenic fungus Fonsecaea pedrosoi, cultivated at 22º C and 36º Cinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis55500500500500246491reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/060e7524-5687-415c-9978-151657c346b9/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811http://repositorio.bc.ufg.br/tede/bitstreams/827ce493-5e98-49cb-b336-1abdfc161e50/downloade39d27027a6cc9cb039ad269a5db8e34MD52ORIGINALDissertação - Davi Vinícius de Lima - 2017.pdfDissertação - Davi Vinícius de Lima - 2017.pdfapplication/pdf1647183http://repositorio.bc.ufg.br/tede/bitstreams/be6e1a0e-c2e6-46ad-bbf2-20a7db1b24a6/download0edd6ec967010bf7bef4a485cc98fbe6MD53tede/105792020-09-08 09:07:29.34http://creativecommons.org/licenses/by-nc-nd/3.0/br/Attribution-NonCommercial-NoDerivs 3.0 Brazilopen.accessoai:repositorio.bc.ufg.br:tede/10579http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2020-09-08T12:07:29Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C |
dc.title.alternative.eng.fl_str_mv |
Proteomic analysis of the human pathogenic fungus Fonsecaea pedrosoi, cultivated at 22º C and 36º C |
title |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C |
spellingShingle |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C Lima, Davi Vinícius de Cromoblastomicose Proteoma Secretoma Virulência Chromoblastomycosis Proteome Virulence CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
title_short |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C |
title_full |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C |
title_fullStr |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C |
title_full_unstemmed |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C |
title_sort |
Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C |
author |
Lima, Davi Vinícius de |
author_facet |
Lima, Davi Vinícius de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bailão, Alexandre Melo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5415221996976886 |
dc.contributor.referee1.fl_str_mv |
Bailão, lexandre Melo |
dc.contributor.referee2.fl_str_mv |
Paccez, Juliano Domiraci |
dc.contributor.referee3.fl_str_mv |
Novaes, Evandro |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8201464584315371 |
dc.contributor.author.fl_str_mv |
Lima, Davi Vinícius de |
contributor_str_mv |
Bailão, Alexandre Melo Bailão, lexandre Melo Paccez, Juliano Domiraci Novaes, Evandro |
dc.subject.por.fl_str_mv |
Cromoblastomicose Proteoma Secretoma Virulência |
topic |
Cromoblastomicose Proteoma Secretoma Virulência Chromoblastomycosis Proteome Virulence CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
dc.subject.eng.fl_str_mv |
Chromoblastomycosis Proteome Virulence |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS |
description |
The Polymorphic fungus Fonsecaea pedrosoi is the main etiological agent of Chromoblastomycosis (CBM), characterized as a chronic cutaneous mycotic infection, occurring mainly in tropical regions. The limited availability of effective therapeutic protocols coupled with CBM clinical polymorphism lead to often prolonged and high relapse rate therapies. Results regarding the molecular biology of F. pedrosoi are scarce however, some virulence factors have already been described, evidencing the need to explore the mechanisms used by this pathogen during infection. The present work carried out in silico analyzes under specific culture conditions, with temperatures of 22º C and 36º C, aiming to better understand the proteome of this fungus, both intracellular and secreted. In order to identify these proteins, we used bioinformatic tools that are available online and allowed the prediction of protein sets that can be secreted by classical and non-classical routes. After the in silico prediction method, proteins were identified in the fungal extracts by the Ultraperformance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MSE) methodology. Those identified in the extracts that brought a more reliable interpretation corroborated the results in silico. The proteins identified stand out for being related to various biological processes, such as metabolism of carbon compounds, energy, proteins related to thermal and oxidative stress, defense, proteins related to the cell cycle, among other functions. The analyzes showed that the vast majority of identified proteins are secreted by alternative pathways, and the results point to the great importance of the biological roles that these secreted proteins can play during the onset of infection. Thus, we observed that the fungus presents variations in protein expression levels according to the condition addressed and may reflect on its behavior and responses to the successful establishment of the infection. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-09-11 |
dc.date.accessioned.fl_str_mv |
2020-09-08T12:07:29Z |
dc.date.available.fl_str_mv |
2020-09-08T12:07:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, D. V. Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C. 2017. 51 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/10579 |
identifier_str_mv |
LIMA, D. V. Análise proteômica do fungo patogênico humano Fonsecaea pedrosoi, cultivado nas condições de temperatura de 22º C e 36º C. 2017. 51 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/10579 |
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por |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Universidade Federal de Goiás |
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Programa de Pós-graduação em Genética e Biologia Molecular (ICB) |
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UFG |
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Brasil |
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Instituto de Ciências Biológicas - ICB (RG) |
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Universidade Federal de Goiás |
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