Desenvolvimento de métodos baseados em espectrometria de massas e cromatografia líquida para análise de compostos químicos produzidos por bioconversão de glicerina

• Ano de defesa: 2018
• Autor(a) principal: Vieira, Flávia Soares
• Orientador(a): Abdelnur, Patrícia Verardi
• Banca de defesa: Abdelnur, Patrícia Verardi, Rodrigues, Clenilson Martins, Kato, Lucília, Correia, Mauro Vicentini
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Química (IQ)
• Departamento: Instituto de Química - IQ (RG)
• País: Brasil
• Palavras-chave em Português: Ácidos orgânicos; Polióis; UHPLC; Espectrometria de massas; Biotecnologia
• Palavras-chave em Inglês: Organic acids; Polyols; Mass spectrometry; Biotechnology
• Área do conhecimento CNPq: QUIMICA::QUIMICA ANALITICA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/8883

Biodiesel production has been increasing every year, which has generated surpluses of glycerin, the main co-product of the transesterification reaction. In the search for applications to this surplus, microbial fermentation has been highlighted as an alternative to add value to this co- product. In this process, microorganisms are used to convert this abundant carbon source into compounds of high economic value, including organic acids and polyols. However, there are still some technological challenges, such as low yield during the fermentation processes and high dependence of new analytical methods, more sensitive and selective, able to identify and quantify the compounds produced. The objective of this work was to establish new analytical methodologies to identify and quantify compounds from bioconversion processes involving the production of organic acids and polyols. To guarantee the quantification of the target compounds, methods based on ultra-high- performance liquid chromatography (UHPLC) were developed and the photodiode array (PDA) and mass spectrometry (MS) detectors were evaluated as alternatives for the monitoring of these two classes of chemical compounds. The analytes investigated in this study were short chain organic acids: citric acid, fumaric acid, glyceric acid, kojic acid, lactic acid, muconic acid, oxalic acid, propionic acid, succinic acid, xylonic acid; and polyols with spatial isomers, including polyols with 4 carbons (C4: erythritol and threitol), 5 carbons (C5: arabitol, ribitol, xylitol), and 6 carbons (C6: dulcitol, iditol, mannitol, sorbitol) and 7 carbons (C7: volemitol). The UHPLC-PDA method developed for the analysis of organic acids was validated by parameters of linearity, limit of detection and quantification, precision and accuracy. The analytical curves showed good linearity with determination coefficients (R²) higher than 0.999 for all organic acids. Precision and accuracy were adequate with intra-day and inter-day variation coefficients below 4.06% and recovery ranged from 94.90% to 109.63%. The developed UHPLC- MS methods presented greater sensitivity and selectivity in the detection of organic acids and polyols, being able to monitor these analytes even in low quantity. The analytical methods developed in this work are crucial tools in the selection or screening of promising microorganisms for low added value substrates conversion and important to optimize biotechnological processes for production of these compounds of interest.