Micropropagação de uvaieira (Eugenia pyriformis Cambess

• Ano de defesa: 2006
• Autor(a) principal: Nascimento, Aline da Costa
• Orientador(a): Paiva, Renato
• Banca de defesa: Costa Netto, Antônio Paulino da, Dutra, Leonardo Ferreira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: UNIVERSIDADE FEDERAL DE LAVRAS
• Programa de Pós-Graduação: DAG - Programa de Pós-graduação
• País: BRASIL
• Palavras-chave em Português: Agronomia; Mata Atlântica; Tecidos (Anatomia e fisiologia) -Cultura e meios de cultura; Frutífera; Micropropagação; Mirtácea; Tissue culture; Micropropagation; Agronomy
• Área do conhecimento CNPq: CNPQ_NÃO_INFORMADO
• Link de acesso: https://repositorio.ufla.br/handle/1/3213

The species Eugenia pyriformis Cambess presents potential to be explored as a fruity and ornamental plant as well as in environmental preservation. The objective of this research was to establish a protocol for its micropropagation. Higher effectiveness in the seed disinfestation process is achieved when seeds are treated with sodium hypochlorite after their removal from tegument. The concentration of salts on WPM and MS medium limits the germination, which is maximized when the seeds are inoculated in MS. The use of GA3 is not required and the addition of 15 g L-1 sucrose is recommended. Higher plant development and vigor are obtained when seeds germinate using sand and vermiculite as substrates. Even though the use of BAP had no effect on shoot induction, higher average number of leaves and buds, the length of the highest shoot and higher shoot number were obtained in the presence of 1.0 mg L-1 BAP. The use of NAA and IBA were not effective on root formation which was observed in the absence of growth regulators. Pre acclimatization in growth room increases the rate of plant survival and the gradual opening of the tube plastic wrapper turned to be a viable acclimatization process. For callus formation, the presence of picloram is essential and no callus was observed in the absence of growth regulators. The interaction between picloram and kinetin was important to increase callus formation from leaf explants and the most effective combination was 0.1 mg L-1 kinetin +1.0 mg L-1 picloran. Maximization of callus formation from shoot segments was obtained in the absence of kinetin and presence of 0.5 mg L-1 picloram. The use of 2,4-D had no effect on callus induction in leaf and shoot explants.