Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process

Detalhes bibliográficos
Ano de defesa: 2025
Autor(a) principal: Santos, Jean Carlos dos lattes
Outros Autores: https://orcid.org/0009-0008-7675-6804
Orientador(a): Ramos, Eduardo Mendes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Lavras
Escola de Ciências Agrárias de Lavras (ESAL)
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciência dos Alimentos
Departamento: Departamento de Ciência dos Alimentos
País: brasil
Palavras-chave em Português:
Área do conhecimento CNPq:
Link de acesso: https://repositorio.ufla.br/handle/1/60405
Resumo: This study aimed to develop an innovative approach for the production of dry-aged beef, named steak dry aging (StDA), by applying the stepwise dry/wet aging method to steaks from Nelore and F1 Angus×Nelore cattle, and evaluating its effects on technological, microbiological, and amino acid quality. Additionally, the impact of prior freezing on the performance of the StDA protocol was investigated to verify its potential to accelerate the process while maintaining the technological and microbiological viability of dry-aged beef. In the first article, beef longissimus thoracis samples were subjected to four treatments: non aged (NMat), dry-aged for 28 days (DA), and two StDA protocols in which steaks were first dry-aged (until an average 15% mass loss) and subsequently wet-aged for 14 days (StDA14) or 28 days (StDA28). In the second article, the effect of pre-freezing and thawing on the StDA protocol was evaluated for both breeds, comparing six treatments: NMat, DA, and four StDA protocols — two without freezing (NF) and two with freezing (FT), each aged for 14 (NF14d and FT14d) or 28 days (NF28d and FT28d). The StDA protocol eliminated the need for trimming, resulting (P < 0.05) in at least a 35% increase in final yield compared to DA. DA samples exhibited higher pH, myofibrillar fragmentation index (51.18 vs. 37.68), and cooking losses (21.58 vs. 17.66%), as well as lower shear force (37.45 vs. 35.54 N) than StDA. Despite a darker and less intense color, no significant differences were observed in total free amino acid content, proximate composition, lipid oxidation, water activity, or total bacterial count. The amino acid profile was similar among aged samples, with a higher proportion of glutamine in StDA28 and taurine in DA. Pre-freezing accelerated the mass loss during the initial dry-aging stage, reducing by one day the time required to reach the target dehydration level while maintaining an 83% yield in StDA samples. Lipid oxidation in FT28d samples (P > 0.05) was similar to DA (~0.38 mg MDA/kg), with higher oxidation in DA samples from F1 Angus than from Nelore. Freezing intensified proteolysis (higher MFI) in StDA samples, resulting in shear force values comparable to DA. Total bacterial counts were lower in NF14d and FT14d and similar among NF28d, FT28d, and DA. A brownish color, associated with a higher metmyoglobin proportion, was observed in NF14d and FT14d samples. NF28d and FT28d samples showed similar hue (h*) values but were darker (lower L*) and less intense (lower C*) than DA. It is concluded that pre-freezing can be incorporated into the StDA process as a strategy to shorten the drying phase and enhance proteolysis while maintaining technological and microbiological viability. The StDA protocol with a total aging period of 28 days, with or without pre-freezing, is a technically feasible and scalable alternative that preserves the quality of dry-aged beef while providing a substantial increase in industrial yield.
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spelling Lopes, Leandro SâmiaRamos, Alcinéia de Lemos SouzaLopes, Leandro SâmiaFontes, Paulo RogérioRamos, Eduardo Mendeshttps://lattes.cnpq.br/0093459005947139Santos, Jean Carlos doshttps://orcid.org/0009-0008-7675-68042025-10-15T19:41:39Z2025-09-05SANTOS, Jean Carlos dos. Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process. 2025. 97 f. Dissertação (Mestrado em Ciência dos Alimentos) — Universidade Federal de Lavras, Lavras, 2025.https://repositorio.ufla.br/handle/1/60405This study aimed to develop an innovative approach for the production of dry-aged beef, named steak dry aging (StDA), by applying the stepwise dry/wet aging method to steaks from Nelore and F1 Angus×Nelore cattle, and evaluating its effects on technological, microbiological, and amino acid quality. Additionally, the impact of prior freezing on the performance of the StDA protocol was investigated to verify its potential to accelerate the process while maintaining the technological and microbiological viability of dry-aged beef. In the first article, beef longissimus thoracis samples were subjected to four treatments: non aged (NMat), dry-aged for 28 days (DA), and two StDA protocols in which steaks were first dry-aged (until an average 15% mass loss) and subsequently wet-aged for 14 days (StDA14) or 28 days (StDA28). In the second article, the effect of pre-freezing and thawing on the StDA protocol was evaluated for both breeds, comparing six treatments: NMat, DA, and four StDA protocols — two without freezing (NF) and two with freezing (FT), each aged for 14 (NF14d and FT14d) or 28 days (NF28d and FT28d). The StDA protocol eliminated the need for trimming, resulting (P < 0.05) in at least a 35% increase in final yield compared to DA. DA samples exhibited higher pH, myofibrillar fragmentation index (51.18 vs. 37.68), and cooking losses (21.58 vs. 17.66%), as well as lower shear force (37.45 vs. 35.54 N) than StDA. Despite a darker and less intense color, no significant differences were observed in total free amino acid content, proximate composition, lipid oxidation, water activity, or total bacterial count. The amino acid profile was similar among aged samples, with a higher proportion of glutamine in StDA28 and taurine in DA. Pre-freezing accelerated the mass loss during the initial dry-aging stage, reducing by one day the time required to reach the target dehydration level while maintaining an 83% yield in StDA samples. Lipid oxidation in FT28d samples (P > 0.05) was similar to DA (~0.38 mg MDA/kg), with higher oxidation in DA samples from F1 Angus than from Nelore. Freezing intensified proteolysis (higher MFI) in StDA samples, resulting in shear force values comparable to DA. Total bacterial counts were lower in NF14d and FT14d and similar among NF28d, FT28d, and DA. A brownish color, associated with a higher metmyoglobin proportion, was observed in NF14d and FT14d samples. NF28d and FT28d samples showed similar hue (h*) values but were darker (lower L*) and less intense (lower C*) than DA. It is concluded that pre-freezing can be incorporated into the StDA process as a strategy to shorten the drying phase and enhance proteolysis while maintaining technological and microbiological viability. The StDA protocol with a total aging period of 28 days, with or without pre-freezing, is a technically feasible and scalable alternative that preserves the quality of dry-aged beef while providing a substantial increase in industrial yield.O presente trabalho teve como objetivo desenvolver uma abordagem inovadora para a produção de carne bovina maturada a seco, denominada steakdry aging (StDA), aplicando o método stepwisedry/wet aging em bifes de bovinos Nelore e cruzas Nelore×Angus (F1 Angus), avaliando seus efeitos sobre a qualidade tecnológica, microbiológica e o perfil de aminoácidos. Adicionalmente, investigou-se o impacto do congelamento prévio sobre o desempenho do protocolo StDA, visando verificar seu potencial para acelerar o processo e manter a viabilidade tecnológica e microbiológica da carne maturada. No primeiro artigo, contrafilés foram submetidos a quatro tratamentos: não maturada (NMat), maturada a seco por 28 dias (DA) e dois protocolos StDA, nos quais bifes foram maturados a seco (até perda média de 15% de massa) e depois a úmido por 14 dias (StDA14) ou 28 dias (StDA28). No segundo artigo, avaliou-se o efeito do congelamento/descongelamento prévio no StDA, comparando-se seis tratamentos: NMat, DA e quatro protocolos StDA, sendo dois com congelamento (FT) e dois sem (NF), maturados por 14 (NF14d e FT14d) ou 28 dias (NF28d e FT28d). O protocolo StDA eliminou a necessidade de toalete, resultando (P < 0,05) em aumento mínimo de 35% no rendimento final em relação ao DA. As amostras DA apresentaram maior pH, índice de fragmentação miofibrilar (51,18 vs 37,68) e perdas por cozimento (21,58 vs 17,66%), além de menor força de cisalhamento (37,45 vs 35,54) que as StDA. Apesar da cor mais escura, não houve diferenças significativas no conteúdo total de aminoácidos livres, composição centesimal, oxidação lipídica, atividade de água ou contagem total de bactérias. O perfil de aminoácidos foi semelhante, com maior proporção de glutamina em StDA28 e de taurina em DA. O congelamento prévio acelerou a perda de massa na etapa inicial de maturação a seco, reduzindo em um dia o tempo necessário para atingir a meta de desidratação e mantendo rendimento de 83% nas amostras StDA. A oxidação lipídica das amostras FT28d foi (P > 0,05) semelhante à de DA (~0,38 mg MAD/kg), sendo maior nas DA de F1 Angus que nas de Nelore. O congelamento intensificou a proteólise (IFM elevado) nas amostras StDA, resultando em força de cisalhamento similar à do DA. A contagem microbiana total foi menor em NF14d e FT14d e semelhante entre NF28d, FT28d e DA. Observou-se coloração amarronzada (maior metamioglobina) em NF14d e FT14d. As amostras NF28d e FT28d apresentaram tonalidade (valor h) similar, porém mais escuras (menor L*) e menos intensas (menor C*) que DA. Conclui-se que o congelamento pode ser incorporado ao processo StDA como estratégia para reduzir o tempo de secagem e potencializar a proteólise, mantendo a viabilidade tecnológica e microbiológica. O protocolo StDA com 28 dias totais, com ou sem congelamento prévio, é uma alternativa tecnicamente viável e escalável, capaz de manter a qualidade da carne maturada semelhante ao processo DA convencional, com expressivo ganho de rendimento industrial.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Arquivo retido a pedido da autoria, até outubro de 2026.SociaisEconômicosTecnológicoCulturaisTecnologia e produçãoODS 8: Trabalho decente e crescimento econômicoODS 9: Indústria, inovação e infraestruturaODS 12: Consumo e produção responsáveisUniversidade Federal de LavrasEscola de Ciências Agrárias de Lavras (ESAL)Programa de Pós-Graduação em Ciência dos AlimentosUFLAbrasilDepartamento de Ciência dos AlimentosAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessCiências AgráriasCarne bovinaMaturação da carneQualidade da carneCongelamento de alimentosCarne congeladaAminoácidosBeefMeat agingMeat qualityAmino acidsFood freezingSteak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging processSteak dry aging: uma estratégia para reduzir perdas por aparas e melhorar o rendimento da carne bovina maturada a secoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAORIGINALTexto completoTexto completoapplication/pdf2055927https://repositorio.ufla.br/bitstreams/0745d74f-6c30-460a-8e29-07480f669d2e/download061c431c35b8c1dde3ba55e7f1171a9eMD51trueAnonymousREAD2026-10-14Impacto da pesquisaImpacto da pesquisaapplication/pdf177283https://repositorio.ufla.br/bitstreams/e0e48ba2-76b4-417e-b2b7-5a58d7879055/download1bb5b9ec51406d301a423d36ef83fe47MD52falseAnonymousREAD2026-10-14CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81025https://repositorio.ufla.br/bitstreams/b460f2fd-5df6-4dd8-8dec-810a193b2643/download5a033ee506f3a0a175bee8fc81f0bd66MD53falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-8955https://repositorio.ufla.br/bitstreams/0bfe5006-d25b-4be4-818e-da5807a6d35e/downloaddc1a173fe9489e283d3a1f54f6ab2ab9MD54falseAnonymousREAD1/604052025-10-15 16:41:41.646http://creativecommons.org/licenses/by/3.0/br/Attribution 3.0 Brazilembargo2026-10-14oai:repositorio.ufla.br:1/60405https://repositorio.ufla.brRepositório InstitucionalPUBhttps://repositorio.ufla.br/server/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2025-10-15T19:41:41Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)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
dc.title.none.fl_str_mv Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
dc.title.alternative.none.fl_str_mv Steak dry aging: uma estratégia para reduzir perdas por aparas e melhorar o rendimento da carne bovina maturada a seco
title Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
spellingShingle Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
Santos, Jean Carlos dos
Ciências Agrárias
Carne bovina
Maturação da carne
Qualidade da carne
Congelamento de alimentos
Carne congelada
Aminoácidos
Beef
Meat aging
Meat quality
Amino acids
Food freezing
title_short Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
title_full Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
title_fullStr Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
title_full_unstemmed Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
title_sort Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process
author Santos, Jean Carlos dos
author_facet Santos, Jean Carlos dos
https://orcid.org/0009-0008-7675-6804
author_role author
author2 https://orcid.org/0009-0008-7675-6804
author2_role author
dc.contributor.co-advisor.none.fl_str_mv Lopes, Leandro Sâmia
dc.contributor.referee.none.fl_str_mv Ramos, Alcinéia de Lemos Souza
Lopes, Leandro Sâmia
Fontes, Paulo Rogério
dc.contributor.advisor1.fl_str_mv Ramos, Eduardo Mendes
dc.contributor.authorLattes.fl_str_mv https://lattes.cnpq.br/0093459005947139
dc.contributor.author.fl_str_mv Santos, Jean Carlos dos
https://orcid.org/0009-0008-7675-6804
contributor_str_mv Ramos, Eduardo Mendes
dc.subject.cnpq.fl_str_mv Ciências Agrárias
topic Ciências Agrárias
Carne bovina
Maturação da carne
Qualidade da carne
Congelamento de alimentos
Carne congelada
Aminoácidos
Beef
Meat aging
Meat quality
Amino acids
Food freezing
dc.subject.por.fl_str_mv Carne bovina
Maturação da carne
Qualidade da carne
Congelamento de alimentos
Carne congelada
Aminoácidos
Beef
Meat aging
Meat quality
Amino acids
Food freezing
description This study aimed to develop an innovative approach for the production of dry-aged beef, named steak dry aging (StDA), by applying the stepwise dry/wet aging method to steaks from Nelore and F1 Angus×Nelore cattle, and evaluating its effects on technological, microbiological, and amino acid quality. Additionally, the impact of prior freezing on the performance of the StDA protocol was investigated to verify its potential to accelerate the process while maintaining the technological and microbiological viability of dry-aged beef. In the first article, beef longissimus thoracis samples were subjected to four treatments: non aged (NMat), dry-aged for 28 days (DA), and two StDA protocols in which steaks were first dry-aged (until an average 15% mass loss) and subsequently wet-aged for 14 days (StDA14) or 28 days (StDA28). In the second article, the effect of pre-freezing and thawing on the StDA protocol was evaluated for both breeds, comparing six treatments: NMat, DA, and four StDA protocols — two without freezing (NF) and two with freezing (FT), each aged for 14 (NF14d and FT14d) or 28 days (NF28d and FT28d). The StDA protocol eliminated the need for trimming, resulting (P < 0.05) in at least a 35% increase in final yield compared to DA. DA samples exhibited higher pH, myofibrillar fragmentation index (51.18 vs. 37.68), and cooking losses (21.58 vs. 17.66%), as well as lower shear force (37.45 vs. 35.54 N) than StDA. Despite a darker and less intense color, no significant differences were observed in total free amino acid content, proximate composition, lipid oxidation, water activity, or total bacterial count. The amino acid profile was similar among aged samples, with a higher proportion of glutamine in StDA28 and taurine in DA. Pre-freezing accelerated the mass loss during the initial dry-aging stage, reducing by one day the time required to reach the target dehydration level while maintaining an 83% yield in StDA samples. Lipid oxidation in FT28d samples (P > 0.05) was similar to DA (~0.38 mg MDA/kg), with higher oxidation in DA samples from F1 Angus than from Nelore. Freezing intensified proteolysis (higher MFI) in StDA samples, resulting in shear force values comparable to DA. Total bacterial counts were lower in NF14d and FT14d and similar among NF28d, FT28d, and DA. A brownish color, associated with a higher metmyoglobin proportion, was observed in NF14d and FT14d samples. NF28d and FT28d samples showed similar hue (h*) values but were darker (lower L*) and less intense (lower C*) than DA. It is concluded that pre-freezing can be incorporated into the StDA process as a strategy to shorten the drying phase and enhance proteolysis while maintaining technological and microbiological viability. The StDA protocol with a total aging period of 28 days, with or without pre-freezing, is a technically feasible and scalable alternative that preserves the quality of dry-aged beef while providing a substantial increase in industrial yield.
publishDate 2025
dc.date.accessioned.fl_str_mv 2025-10-15T19:41:39Z
dc.date.issued.fl_str_mv 2025-09-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv SANTOS, Jean Carlos dos. Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process. 2025. 97 f. Dissertação (Mestrado em Ciência dos Alimentos) — Universidade Federal de Lavras, Lavras, 2025.
dc.identifier.uri.fl_str_mv https://repositorio.ufla.br/handle/1/60405
identifier_str_mv SANTOS, Jean Carlos dos. Steak dry aging: a strategy to reduce trimming losses and improve yield in beef dry aging process. 2025. 97 f. Dissertação (Mestrado em Ciência dos Alimentos) — Universidade Federal de Lavras, Lavras, 2025.
url https://repositorio.ufla.br/handle/1/60405
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Escola de Ciências Agrárias de Lavras (ESAL)
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciência dos Alimentos
dc.publisher.initials.fl_str_mv UFLA
dc.publisher.country.fl_str_mv brasil
dc.publisher.department.fl_str_mv Departamento de Ciência dos Alimentos
publisher.none.fl_str_mv Universidade Federal de Lavras
Escola de Ciências Agrárias de Lavras (ESAL)
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