Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina

Detalhes bibliográficos
Ano de defesa: 2001
Autor(a) principal: Josely Ferreira Figueiredo
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Bovino Doenças
Infecções por campylobacter
Imunofluorescência
Bovino
Lavado prepucial
Diagnóstico
Campylobacter fetus
Campilobacteriose genital bovina
Imunoflurescência direta
Link de acesso: https://hdl.handle.net/1843/BUOS-8BZG64
Resumo: The direct immunofluorescence test for the diagnosis of Bovine Genital Campylobacteriosis was evaluated for detection limit, effect of observers, sensitivity and specificity. Furthermore, the specificity of the fluorescent conjugate was tested against Campylobacter SP. Arcobacter SP. Helicohacter SP. E. coli bacteria and normal flora of precúcio. Ten dilutions were used at the base of 10 C. fetus subsp. Venerealis NCTC 10354 or washed in PBS and centrifuged preputial not centrifuged. Every experiment was done blindly, in duplicate, with positive and negative controls and three observers. The detection limits of direct immunofluorescence were 104, 104 and 102 CFU / ml. Respectively, in PBS, centrifuged and washed preputial not centrifuged. There were no significant differences between the readings of the observers. The technique had a specificity of 88.89% and sensitivity of 92.59%. The fluorescent conjugate recognized only C. fetus. Direct immunofluorescence for C. fetus subsp. Veneralia proved to be sensitive and specific was not influenced by the observers
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spelling Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovinaBovino DoençasInfecções por campylobacterImunofluorescênciaBovinoLavado prepucialDiagnósticoCampylobacter fetusCampilobacteriose genital bovinaImunoflurescência diretaThe direct immunofluorescence test for the diagnosis of Bovine Genital Campylobacteriosis was evaluated for detection limit, effect of observers, sensitivity and specificity. Furthermore, the specificity of the fluorescent conjugate was tested against Campylobacter SP. Arcobacter SP. Helicohacter SP. E. coli bacteria and normal flora of precúcio. Ten dilutions were used at the base of 10 C. fetus subsp. Venerealis NCTC 10354 or washed in PBS and centrifuged preputial not centrifuged. Every experiment was done blindly, in duplicate, with positive and negative controls and three observers. The detection limits of direct immunofluorescence were 104, 104 and 102 CFU / ml. Respectively, in PBS, centrifuged and washed preputial not centrifuged. There were no significant differences between the readings of the observers. The technique had a specificity of 88.89% and sensitivity of 92.59%. The fluorescent conjugate recognized only C. fetus. Direct immunofluorescence for C. fetus subsp. Veneralia proved to be sensitive and specific was not influenced by the observersUniversidade Federal de Minas Gerais2019-08-12T03:46:53Z2025-09-08T23:03:02Z2019-08-12T03:46:53Z2001-03-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://hdl.handle.net/1843/BUOS-8BZG64Josely Ferreira Figueiredoinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG2025-09-08T23:03:02Zoai:repositorio.ufmg.br:1843/BUOS-8BZG64Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-09-08T23:03:02Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
title Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
spellingShingle Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
Josely Ferreira Figueiredo
Bovino Doenças
Infecções por campylobacter
Imunofluorescência
Bovino
Lavado prepucial
Diagnóstico
Campylobacter fetus
Campilobacteriose genital bovina
Imunoflurescência direta
title_short Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
title_full Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
title_fullStr Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
title_full_unstemmed Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
title_sort Avaliação da imunofluorescência direta no diagnóstico da campilobacteriose genital bovina
author Josely Ferreira Figueiredo
author_facet Josely Ferreira Figueiredo
author_role author
dc.contributor.author.fl_str_mv Josely Ferreira Figueiredo
dc.subject.por.fl_str_mv Bovino Doenças
Infecções por campylobacter
Imunofluorescência
Bovino
Lavado prepucial
Diagnóstico
Campylobacter fetus
Campilobacteriose genital bovina
Imunoflurescência direta
topic Bovino Doenças
Infecções por campylobacter
Imunofluorescência
Bovino
Lavado prepucial
Diagnóstico
Campylobacter fetus
Campilobacteriose genital bovina
Imunoflurescência direta
description The direct immunofluorescence test for the diagnosis of Bovine Genital Campylobacteriosis was evaluated for detection limit, effect of observers, sensitivity and specificity. Furthermore, the specificity of the fluorescent conjugate was tested against Campylobacter SP. Arcobacter SP. Helicohacter SP. E. coli bacteria and normal flora of precúcio. Ten dilutions were used at the base of 10 C. fetus subsp. Venerealis NCTC 10354 or washed in PBS and centrifuged preputial not centrifuged. Every experiment was done blindly, in duplicate, with positive and negative controls and three observers. The detection limits of direct immunofluorescence were 104, 104 and 102 CFU / ml. Respectively, in PBS, centrifuged and washed preputial not centrifuged. There were no significant differences between the readings of the observers. The technique had a specificity of 88.89% and sensitivity of 92.59%. The fluorescent conjugate recognized only C. fetus. Direct immunofluorescence for C. fetus subsp. Veneralia proved to be sensitive and specific was not influenced by the observers
publishDate 2001
dc.date.none.fl_str_mv 2001-03-22
2019-08-12T03:46:53Z
2019-08-12T03:46:53Z
2025-09-08T23:03:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1843/BUOS-8BZG64
url https://hdl.handle.net/1843/BUOS-8BZG64
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv repositorio@ufmg.br
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