TRYPANOSOMA SPP. EM BOVINOS NO PANTANAL DE MATO GROSSO DO SUL, BRASIL

• Ano de defesa: 2021
• Autor(a) principal: Jessica Teles Echeverria
• Orientador(a): Fernando Paiva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Fundação Universidade Federal de Mato Grosso do Sul
• País: Brasil
• Palavras-chave em Português: Ruminantes, Diagnóstico, Epidemiologia, Tripanosomíases
• Link de acesso: https://repositorio.ufms.br/handle/123456789/4047

The real impact of Trypanosoma spp. over time in cattle, it is unknown in Brazil. The objective of this work was to estimate the parasitological, serological and molecular prevalence of Trypanosoma spp. in cattle in the Pantanal of Mato Grosso do Sul, Brazil, more than two decades after its introduction. A total of 312 cattle, of both sexes, predominantly of the Nelore breed, were sampled in four rural properties in the Pantanal region, in the state of Mato Grosso do Sul, adopting the method of stratified random sampling (adults and young people) and in clusters (properties), for diagnoses of Trypanosoma spp. using the techniques of microhematocrit and PCR-FFLB (Fluorescent Fragment Length Barcoding). In the same properties, another 170 samples of adult bovine serum were collected for the Enzyme Immunoassay method. Using the microhematocrit technique, 14.42% (45/312) of the cattle were infected by Trypanosoma spp.; considering the parasitic intensity, 37 were classified as + (1-5 trypomastigotes), 7 as ++ (6-10) and 1 as ++++ (>20). The globular volume in all animals was within the normal range (24 to 46%) for the species and animal category. According to the PCR-FFLB results, 34.61% (108/312) of the animals were carriers of T. theileri and 1.6% (5/312) of the animals had T. vivax. Among the animals sampled, only two showed co-infection for both species. By ELISA, 50.59% (86/170) of the samples were reactive to the T. vivax recombinant antigen used. The species found by PCR-FFLB carrying T. theileri and/or T. vivax were clinically without symptoms. The epidemiological situation of T. Theileri could not determine accurately by the same criteria adopted for the other species, but we can conclude that this species is the most prevalent in bovine animals in the Pantanal. The species T. vivax has a low molecular prevalence, associated with low parasitic intensity, with a high number of individuals with antibodies, in the absence of symptoms, in the Pantanal conditions where vectors for this species are only mechanical, communicability is low and transmission vector is reduced, so it can be concluded that the species T. vivax is in endemic equilibrium with cattle from Pantanal Sul-mato-grossense. Keywords: Diagnosis, Epidemiology, Ruminants, Trypanosomes.