Identificação de flavonoides por LC-MS/MS de extratos de folhas de Caesalpinia ferrea Mart. e avaliação da reversão do estresse oxidativo em camundongos

• Ano de defesa: 2017
• Autor(a) principal: Souza, Camila Cristina Pereira de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Instituto de Ciências Naturais, Humanas e Sociais (ICNHS) – Sinop; UFMT CUS - Sinop; Programa de Pós-Graduação em Ciências Ambientais
• Palavras-chave em Português: Plantas medicinais; Flavonoide; Antioxidante; Paracetamol; Estresse; CNPQ::OUTROS::CIENCIAS; Medicinal plants; Flavonoid; Antioxidant; Acetaminophen; Oxidative stress
• Link de acesso: http://ri.ufmt.br/handle/1/4131

Caesalpinia ferrea is used in folk medicine for the treatment of conditions bronchitis, diabetes, intestinal colic, diarrhea, gingivitis and syphilis. Scientific studies show that C. ferrea has antimicrobial, healing, anti-inflammatory and anti-tumor activity. This work deals with the radical radical DPPH activity and antioxidant activity of ethanolic (EE) and ethyl acetate (EA) extracts of C. ferrea leaves collected in Sinop / MT against oxidative stress induced with paracetamol (PCM) in Swiss mice Males. Leaves of C. ferrea were collected in Sinop / MT to prepare extracts of ethyl acetate (EA) and ethanolic (EE). EA and EE had determined in vitro antioxidant potential by spectrophotometry UV-VIS and flavonoids identified by liquid chromatography-tandem mass spectrometry (LC-MS / MS). In the biological study, the effect of EE and EA (50 mg / kg) compared to controls with water and PCM administration (250 mg / kg) was evaluated in the determination of biomarkers dosage superoxide dismutase (SOD), catalase (GST), reduced glutathione (GSH), carbonylated proteins (PC), ascorbic acid (ASA), thiobarbituric acid derivatives (TBARS) from oxidative stress in hepatic and / or renal tissues. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), glycemia, triglycerides and cholesterol parameters were evaluated. The flavonoids rutin, amentoflavone, quercetin-3-β-D-glucoside, quercetin, taxifoline, apigenin and luteolin were identified in both extracts. Besides these, quercitrin was found in EE. The radical activity of EE and EA demonstrated EC 50 46.0 μg / mL and 34.3 μg / mL, respectively. The activity of liver enzymes was reduced by CAT (37.6%) and GST (20.6%) in the group intoxicated with PCM and EA was hepatoprotective on these enzymes. Rates of CP increased in the PCM group by 76.8% (liver) and 45.2% (kidney). EE and EA reduced this toxic effect of PCM on CP in both tissues evaluated. GSH was depleted 27.1% (hepatic) and 42.4% (renal) in the PCM and EA (liver) groups and EE and AI (kidney) were effective in restoring GSH levels. ASA suffered a reduction of 20.0% in the group exposed to PCM and EA was able to restore these levels. Elevation (44.4%) was observed in the TBARS levels in the PCM group and there were no significant alterations in the other groups. There was an increase in the serum levels of ALT (119.7%) and AST (68.7%) in the group exposed to PCM, however the treatment with EE and EA were effective in reducing these activities. EE and EA were hypolipidemic and EA was hypoglycemic. Therefore, the highest total phenol content of EA added to its best antioxidant potential in vitro also corroborates with its better biological antioxidant effect monitored by the biomarkers evaluated in this study.