MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Carlos , Angélica Cardoso lattes
Orientador(a): Siqueira Junior, José Pinto de
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal da Paraíba
Programa de Pós-Graduação: Programa de Pós-Graduação em Biologia Celular e Molecular
Departamento: Biologia Celular e Molecular
País: Brasil
Palavras-chave em Português:
microRNA
vírus Epstein-Barr
BTRF1
BSRF1
Palavras-chave em Inglês:
microRNA
Epstein-Barr virus
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/9820
Resumo: The microRNAs are of great interest in studies on viral infections. In the context of the Epstein-Barr virus (EBV), they act as important regulators of the expression of viral and cellular genes, facilitating viral persistence and oncogenesis. The present work aims to identify viral microRNAs, their target mRNAs and the essential target proteins specifically for Human gammaherpesvirus 4 tipo 2. First, we used the StructRNAfinder and IntaRNA programs to perform the identification tasks of miRNAs and target mRNAs, respectively. Subsequently, we created a network of proteins from the data obtained from the tool online virusmentha and visualized in the program cytoscape. In addition, we performed a topological analysis of the protein interaction network with the cytoscape tool. We found 55 viral miRNAs and 46 mRNAs and identified 10 proteins that were prominent in the protein interaction network. The BTRF1 and BSFR1 proteins exert a greater control in the protein network, although they are proteins of the integument with unknown functions. Thus, we suggest that the BTRF1 and BSRF1 proteins are potential targets for understanding and controlling EBV infections.
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spelling 2018-04-25T12:06:32Z2018-07-20T23:36:05Z2018-07-20T23:36:05Z2017-10-27CARLOS, Angélica Cardoso. MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas. 2017. 83 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraíba, João Pessoa, 2017.https://repositorio.ufpb.br/jspui/handle/tede/9820The microRNAs are of great interest in studies on viral infections. In the context of the Epstein-Barr virus (EBV), they act as important regulators of the expression of viral and cellular genes, facilitating viral persistence and oncogenesis. The present work aims to identify viral microRNAs, their target mRNAs and the essential target proteins specifically for Human gammaherpesvirus 4 tipo 2. First, we used the StructRNAfinder and IntaRNA programs to perform the identification tasks of miRNAs and target mRNAs, respectively. Subsequently, we created a network of proteins from the data obtained from the tool online virusmentha and visualized in the program cytoscape. In addition, we performed a topological analysis of the protein interaction network with the cytoscape tool. We found 55 viral miRNAs and 46 mRNAs and identified 10 proteins that were prominent in the protein interaction network. The BTRF1 and BSFR1 proteins exert a greater control in the protein network, although they are proteins of the integument with unknown functions. Thus, we suggest that the BTRF1 and BSRF1 proteins are potential targets for understanding and controlling EBV infections.Os microRNAs são de grande interesse em estudos sobre infecções virais. No contexto do vírus Epstein-Barr (EBV), eles atuam como importantes reguladores da expressão de genes virais e celulares, facilitando a persistência viral e a oncogênese. O presente trabalho objetiva identificar microRNAs virais, seus mRNAs alvos e as proteínas alvos essenciais específicas para o Human gammaherpesvirus 4 tipo 2. Primeiramente, utilizamos os programas StructRNAfinder e o IntaRNA para realizar as tarefas de idenficação de miRNAs e mRNAs alvos, respectivamente. Posteriormente, criamos uma rede de proteínas a partir dos dados obtidos da ferramenta online virusmentha e visualizamos no programa cytoscape. Além disso, realizamos uma análise topológica da rede de interação de proteínas com a ferramenta cytoscape. Encontramos 55 miRNAs virais e 46 mRNAs e identificamos 10 proteínas que se destacavam na rede de interação de proteínas. As proteínas BTRF1 e BSFR1 exercem um maior controle na rede de proteínas, embora sejam proteínas do tegumento com funções desconhecidas. Assim, sugerimos que as proteínas BTRF1 e BSRF1 sejam alvos potenciais para compreender e controlar as infecções por EBV.Submitted by Leonardo Cavalcante (leo.ocavalcante@gmail.com) on 2018-04-25T12:06:32Z No. of bitstreams: 1 Arquivototal.pdf: 2117961 bytes, checksum: 458d516added97ab516b1c8ae4ec5ddd (MD5)Made available in DSpace on 2018-04-25T12:06:32Z (GMT). No. of bitstreams: 1 Arquivototal.pdf: 2117961 bytes, checksum: 458d516added97ab516b1c8ae4ec5ddd (MD5) Previous issue date: 2017-10-27Made available in DSpace on 2018-07-20T23:36:05Z (GMT). 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dc.title.por.fl_str_mv MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
title MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
spellingShingle MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
Carlos , Angélica Cardoso
microRNA
vírus Epstein-Barr
BTRF1
BSRF1
BTRF1
BSRF1
microRNA
Epstein-Barr virus
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
title_full MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
title_fullStr MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
title_full_unstemmed MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
title_sort MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas
author Carlos , Angélica Cardoso
author_facet Carlos , Angélica Cardoso
author_role author
dc.contributor.advisor1.fl_str_mv Siqueira Junior, José Pinto de
dc.contributor.advisor-co1.fl_str_mv Farias, Sávio Torres de
dc.contributor.authorID.fl_str_mv 08287399432
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8202485862312409
dc.contributor.author.fl_str_mv Carlos , Angélica Cardoso
contributor_str_mv Siqueira Junior, José Pinto de
Farias, Sávio Torres de
dc.subject.por.fl_str_mv microRNA
vírus Epstein-Barr
BTRF1
BSRF1
BTRF1
BSRF1
topic microRNA
vírus Epstein-Barr
BTRF1
BSRF1
BTRF1
BSRF1
microRNA
Epstein-Barr virus
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv microRNA
Epstein-Barr virus
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description The microRNAs are of great interest in studies on viral infections. In the context of the Epstein-Barr virus (EBV), they act as important regulators of the expression of viral and cellular genes, facilitating viral persistence and oncogenesis. The present work aims to identify viral microRNAs, their target mRNAs and the essential target proteins specifically for Human gammaherpesvirus 4 tipo 2. First, we used the StructRNAfinder and IntaRNA programs to perform the identification tasks of miRNAs and target mRNAs, respectively. Subsequently, we created a network of proteins from the data obtained from the tool online virusmentha and visualized in the program cytoscape. In addition, we performed a topological analysis of the protein interaction network with the cytoscape tool. We found 55 viral miRNAs and 46 mRNAs and identified 10 proteins that were prominent in the protein interaction network. The BTRF1 and BSFR1 proteins exert a greater control in the protein network, although they are proteins of the integument with unknown functions. Thus, we suggest that the BTRF1 and BSRF1 proteins are potential targets for understanding and controlling EBV infections.
publishDate 2017
dc.date.issued.fl_str_mv 2017-10-27
dc.date.accessioned.fl_str_mv 2018-04-25T12:06:32Z
2018-07-20T23:36:05Z
dc.date.available.fl_str_mv 2018-07-20T23:36:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARLOS, Angélica Cardoso. MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas. 2017. 83 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraíba, João Pessoa, 2017.
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/tede/9820
identifier_str_mv CARLOS, Angélica Cardoso. MicroRNA no vírus Epstein-Barr: identificação, predição de alvos e rede de proteínas. 2017. 83 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraíba, João Pessoa, 2017.
url https://repositorio.ufpb.br/jspui/handle/tede/9820
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biologia Celular e Molecular
dc.publisher.initials.fl_str_mv UFPB
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Biologia Celular e Molecular
publisher.none.fl_str_mv Universidade Federal da Paraíba
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPB
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instname_str Universidade Federal da Paraíba (UFPB)
instacron_str UFPB
institution UFPB
reponame_str Repositório Institucional da UFPB
collection Repositório Institucional da UFPB
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