Efeito dos organocalcogênios sobre a atividade da enzima δ-ALA-D sanguínea: estudos in vitro e em humanos expostos ocupacionalmente a estes compostos

• Ano de defesa: 2002
• Autor(a) principal: Corralo, Vanessa da Silva
• Orientador(a): Zeni, Gilson Rogério
• Banca de defesa: Jacob, Raquel Guimarães, Braga, Antonio Luiz
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria; Centro de Ciências Naturais e Exatas
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
• Departamento: Ciências Biológicas
• País: Brasil
• Palavras-chave em Português: Organocalcogênios; Enzimas; Síntese orgânica; Toxicologia; Bioquímica toxicológica; Bioquímica
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
• Link de acesso: http://repositorio.ufsm.br/handle/1/26656

Organochalcogens are important intermediates and the main applied reagents in organic synthesis, which can increase human exposure risk to these chemicals in the workplace. Since, the erythrocytic δ-ALA-D activity could be an important indicator of toxicity this report investigated the organochalcogens effects on blood δ-ALA-D in vitro and determined δ-ALAD activity on human occupationally exposed to organochalcogens. The acute tixicity of organochalcogens on erythrocytic δ-ALA-D activity, in mice, was also investigated. δ-ALA-D activity was assayed according to the method of Berlin & Schaller (1974) with some modifications. Diphenyl ditelluride, diphenyl diselenide and Ebselen inhibited in a concentration-dependent manner δ-ALA-D activity from human erythrocytes. Dithiothreitol (DTT), the hydrophobic SH-reducing agent, was able to reactivate and to protect inhibited δ-ALA-D. The administration of diphenyl diselenide (200 μmol/kg) and diphenyl ditelluride (150 μmol/kg) in a toxic amount inhibited the erythrocytic δ-ALA-D activity on mice. Otherwise, the human occupationally exposed to organochalcogens did not increase selenium levels, from these findings we can not affirm that human δ-ALA-D was a target for organochalcogens ex vivo. In addition δ-ALA-D was a target toxicity from organochalcogens, in vitro. But, mice erytrocytes δ-ALA-D was a target for organochalcogens tested ex vivo.