Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)

Detalhes bibliográficos
Ano de defesa: 2023
Autor(a) principal: Faria, Sophia Iwersen lattes
Orientador(a): Schuch, André Passaglia lattes
Banca de defesa: Segatto, Ana Lúcia Anversa, Vizzoto, Bruno Stefanello
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
Departamento: Bioquímica
País: Brasil
Palavras-chave em Português:
Terapia fotodinâmica
Porfirinas catiônicas
Porfirinas aniônicas
Zn(II)
Dano ao DNA
Melanoma
Palavras-chave em Inglês:
Photodynamic therapy
Cationic porphyrins
Anionic porphyrins
DNA damage
Área do conhecimento CNPq:
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
Link de acesso: http://repositorio.ufsm.br/handle/1/30422
Resumo: Photodynamic therapy (PDT) is a treatment with several benefits, such as few side effects, ease of application and possible combination with other treatments. It is based on the production of reactive oxygen species (ROS) by a Photosensitizer (PS) upon irradiation. Due to their many desirable characteristics, porphyrins are often used as PS in PDT. Some of their attributes can vary, influencing its interaction with biomolecules of interest. In this work, four compounds were studied: TMePyP+, TPPS-, ZnTMe+ and ZnTTP-. Their interactions with DNA were evaluated through genotoxicity, ROS production and cytotoxicity. Results showed that cationic porphyrins were way more effective in inducing DNA damage, generating ROS and reducing cell viability than anionic ones. The zinc addition had little effect on those aspects, but caused the porphyrin to be selective towards tumours cells. Thus, this study provides insight on the role that charge and metal addition play in the interaction with DNA, and also that TMePyP+ and ZnTMe+ are promising candidates for use as PS in PDT.
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spelling 2023-11-01T12:59:17Z2023-11-01T12:59:17Z2023-09-06http://repositorio.ufsm.br/handle/1/30422Photodynamic therapy (PDT) is a treatment with several benefits, such as few side effects, ease of application and possible combination with other treatments. It is based on the production of reactive oxygen species (ROS) by a Photosensitizer (PS) upon irradiation. Due to their many desirable characteristics, porphyrins are often used as PS in PDT. Some of their attributes can vary, influencing its interaction with biomolecules of interest. In this work, four compounds were studied: TMePyP+, TPPS-, ZnTMe+ and ZnTTP-. Their interactions with DNA were evaluated through genotoxicity, ROS production and cytotoxicity. Results showed that cationic porphyrins were way more effective in inducing DNA damage, generating ROS and reducing cell viability than anionic ones. The zinc addition had little effect on those aspects, but caused the porphyrin to be selective towards tumours cells. Thus, this study provides insight on the role that charge and metal addition play in the interaction with DNA, and also that TMePyP+ and ZnTMe+ are promising candidates for use as PS in PDT.A terapia fotodinâmica (TDF) é um tratamento com diversos benefícios, como poucos efeitos colaterais, facilidade de aplicação e possível combinação com outros tratamentos. Baseia-se na produção de espécies reativas de oxigênio (ERO) por um fotossensibilizador (FS) após ser irradiado. Devido às suas muitas características desejáveis, as porfirinas são frequentemente usadas como FS em TFD. Alguns de seus atributos podem variar, influenciando sua interação com biomoléculas de interesse. Neste trabalho, quatro compostos foram estudados: TMePyP+, TPPS-, ZnTMe+ e ZnTTP-. Suas interações com o DNA foram avaliadas através de genotoxicidade, produção de EROs e citotoxicidade. Os resultados mostraram que as porfirinas catiônicas foram muito mais eficazes na indução de danos ao DNA, geração de EROs e redução da viabilidade celular do que as aniônicas. A adição de zinco teve pouco efeito sobre esses aspectos, mas fez com que a porfirina se tornasse mais seletiva para células tumorais. Assim, este estudo fornece informações sobre o papel que a carga e a adição de metal desempenham na interação com o DNA, e também demonstra que TMePyP+ e ZnTMe+ são candidatos promissores para uso como PS na TFD.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTerapia fotodinâmicaPorfirinas catiônicasPorfirinas aniônicasZn(II)Dano ao DNAMelanomaPhotodynamic therapyCationic porphyrinsAnionic porphyrinsDNA damageCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICACaracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)Characterization of genotoxic potential of free base porphyrins and Zn (II) metalloporphyrinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSchuch, André Passagliahttp://lattes.cnpq.br/4932611269622766Segatto, Ana Lúcia AnversaVizzoto, Bruno Stefanellohttp://lattes.cnpq.br/3901259672991234Faria, Sophia Iwersen200800000002600600600600600d93539a4-7b55-44e5-9c20-52601682b4f7f4e926e5-7b94-493f-82f5-12737c15b1911f70f921-07f3-473f-b3f8-7a006f0ffafe2bc792c3-3d91-463d-83d2-6558885975a5reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/30422/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALDIS_PPGBT_2023_FARIA_SOPHIA.pdfDIS_PPGBT_2023_FARIA_SOPHIA.pdfDissertaçãoapplication/pdf3600875http://repositorio.ufsm.br/bitstream/1/30422/1/DIS_PPGBT_2023_FARIA_SOPHIA.pdff79ffa106f4660bbab86f97c08cd6688MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/30422/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD521/304222023-11-01 09:59:17.289oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-11-01T12:59:17Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
dc.title.alternative.eng.fl_str_mv Characterization of genotoxic potential of free base porphyrins and Zn (II) metalloporphyrins
title Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
spellingShingle Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
Faria, Sophia Iwersen
Terapia fotodinâmica
Porfirinas catiônicas
Porfirinas aniônicas
Zn(II)
Dano ao DNA
Melanoma
Photodynamic therapy
Cationic porphyrins
Anionic porphyrins
DNA damage
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_full Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_fullStr Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_full_unstemmed Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_sort Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
author Faria, Sophia Iwersen
author_facet Faria, Sophia Iwersen
author_role author
dc.contributor.advisor1.fl_str_mv Schuch, André Passaglia
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4932611269622766
dc.contributor.referee1.fl_str_mv Segatto, Ana Lúcia Anversa
dc.contributor.referee2.fl_str_mv Vizzoto, Bruno Stefanello
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3901259672991234
dc.contributor.author.fl_str_mv Faria, Sophia Iwersen
contributor_str_mv Schuch, André Passaglia
Segatto, Ana Lúcia Anversa
Vizzoto, Bruno Stefanello
dc.subject.por.fl_str_mv Terapia fotodinâmica
Porfirinas catiônicas
Porfirinas aniônicas
Zn(II)
Dano ao DNA
Melanoma
topic Terapia fotodinâmica
Porfirinas catiônicas
Porfirinas aniônicas
Zn(II)
Dano ao DNA
Melanoma
Photodynamic therapy
Cationic porphyrins
Anionic porphyrins
DNA damage
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
dc.subject.eng.fl_str_mv Photodynamic therapy
Cationic porphyrins
Anionic porphyrins
DNA damage
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Photodynamic therapy (PDT) is a treatment with several benefits, such as few side effects, ease of application and possible combination with other treatments. It is based on the production of reactive oxygen species (ROS) by a Photosensitizer (PS) upon irradiation. Due to their many desirable characteristics, porphyrins are often used as PS in PDT. Some of their attributes can vary, influencing its interaction with biomolecules of interest. In this work, four compounds were studied: TMePyP+, TPPS-, ZnTMe+ and ZnTTP-. Their interactions with DNA were evaluated through genotoxicity, ROS production and cytotoxicity. Results showed that cationic porphyrins were way more effective in inducing DNA damage, generating ROS and reducing cell viability than anionic ones. The zinc addition had little effect on those aspects, but caused the porphyrin to be selective towards tumours cells. Thus, this study provides insight on the role that charge and metal addition play in the interaction with DNA, and also that TMePyP+ and ZnTMe+ are promising candidates for use as PS in PDT.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-11-01T12:59:17Z
dc.date.available.fl_str_mv 2023-11-01T12:59:17Z
dc.date.issued.fl_str_mv 2023-09-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/30422
url http://repositorio.ufsm.br/handle/1/30422
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 200800000002
dc.relation.confidence.fl_str_mv 600
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600
600
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Bioquímica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UFSM
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