Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart.
Ano de defesa: | 2012 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
Programa de Pós-Graduação: |
Não Informado pela instituição
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Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
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Palavras-chave em Português: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11195 |
Resumo: | Oxidative stress has been linked to some neurodegenerative disorders. Current therapies are limited to attenuate the symptoms, however some studies have shown that antioxidant compounds may be able to prevent or delay neuronal oxidative damage, including those present in plant extracts. For these reasons, this study investigated the possible antioxidant activity of the ethanolic extract of Luehea divaricata leaves in brain of rats in vitro and the possible antioxidant mechanisms involved. The extract was evaluated against basal and sodium nitroprusside (SNP) 5 μM induced lipid peroxidation in brain of rats through measurements of thiobarbituric acid reactive substances (TBARS) production. Slices of brain areas were treated with SNP 100 M and extract to determine cellular viability by MTT reduction assay. Scavenger activity was evaluated against NO, DPPH and OH through Griess reagent, DPPH and deoxyribose oxidation assays, respectively. The chelating and reducing capacity for iron were determined by the orto-phenantroline method. The extract was screened by HPLC for the presence of gallic, chlorogenic, and caffeic acids, quercetin, rutin, and kaempferol. Only rutin was detected and then was used, in the same concentrations of the extract, as standard in all assays. L. divaricata extract (1-10 μg/ml) protected against induced lipid peroxidation, decreased basal levels of TBARS (about 50%) and maintained the cells viable. The extract was not able to protect deoxyribose against OH and to chelate iron, however it inhibited NO and DPPH in 33.14% at 20 μg/ml and 53.93% at 50 μg/ml respectively, and showed a reducing power in a concentration and time dependent manner. Therefore, L. divaricata ethanolic leaf extract showed antioxidant properties in vitro at low concentrations. The antioxidant mechanisms were related to scavenger activity on reactive oxygen and nitrogen species and metal reducing property. These effects were similar or more powerful than rutin in same concentrations in all assays, except in NO scavenger activity, and, thus, other unidentified compounds present in the extract appear to be associated with the effects observed in this study. More studies are needed regarding the identification of these compounds and the neuroprotective activity of the extract. |
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Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart.In vitro antioxidant activity of the ethanolic extract of Luehea divaricata Mart. leavesAçoita-cavaloCompostos fenólicosEspécies reativas de nitrogênioEspécies reativas de oxigênioExtrato vegetalNeuroproteçãoAçoita-cavaloNeuroprotectionPhenolic compoundsPlant extractReactive oxygen speciesReactive nitrogen speciesCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAOxidative stress has been linked to some neurodegenerative disorders. Current therapies are limited to attenuate the symptoms, however some studies have shown that antioxidant compounds may be able to prevent or delay neuronal oxidative damage, including those present in plant extracts. For these reasons, this study investigated the possible antioxidant activity of the ethanolic extract of Luehea divaricata leaves in brain of rats in vitro and the possible antioxidant mechanisms involved. The extract was evaluated against basal and sodium nitroprusside (SNP) 5 μM induced lipid peroxidation in brain of rats through measurements of thiobarbituric acid reactive substances (TBARS) production. Slices of brain areas were treated with SNP 100 M and extract to determine cellular viability by MTT reduction assay. Scavenger activity was evaluated against NO, DPPH and OH through Griess reagent, DPPH and deoxyribose oxidation assays, respectively. The chelating and reducing capacity for iron were determined by the orto-phenantroline method. The extract was screened by HPLC for the presence of gallic, chlorogenic, and caffeic acids, quercetin, rutin, and kaempferol. Only rutin was detected and then was used, in the same concentrations of the extract, as standard in all assays. L. divaricata extract (1-10 μg/ml) protected against induced lipid peroxidation, decreased basal levels of TBARS (about 50%) and maintained the cells viable. The extract was not able to protect deoxyribose against OH and to chelate iron, however it inhibited NO and DPPH in 33.14% at 20 μg/ml and 53.93% at 50 μg/ml respectively, and showed a reducing power in a concentration and time dependent manner. Therefore, L. divaricata ethanolic leaf extract showed antioxidant properties in vitro at low concentrations. The antioxidant mechanisms were related to scavenger activity on reactive oxygen and nitrogen species and metal reducing property. These effects were similar or more powerful than rutin in same concentrations in all assays, except in NO scavenger activity, and, thus, other unidentified compounds present in the extract appear to be associated with the effects observed in this study. More studies are needed regarding the identification of these compounds and the neuroprotective activity of the extract.Conselho Nacional de Desenvolvimento Científico e TecnológicoO estresse oxidativo tem sido relacionado a algumas desordens neurodegenerativas. As terapias atuais para essas doenças estão limitadas a atenuar os sintomas apresentados, entretanto alguns estudos têm mostrado que compostos antioxidantes podem ser capazes de prevenir ou retardar o dano oxidativo neuronal, incluindo aqueles presentes em extratos vegetais. Por esses motivos, neste trabalho foi investigada a possível atividade antioxidante do extrato etanólico das folhas de Luehea divaricata em cérebro de ratos in vitro e os possíveis mecanismos antioxidantes envolvidos. O extrato foi avaliado contra a peroxidação lipídica basal e induzida por nitroprussiato de sódio (NPS) 5 μM em cérebro de ratos através da quantificação da produção de espécies reativas ao ácido tiobarbitúrico (TBARS). Fatias de áreas do cérebro foram tratadas com NPS 100 M e extrato para determinar a viabilidade celular através do ensaio da redução do MTT. A atividade scavenger do extrato foi testada contra ON, DPPH e OH através do reagente de Griess e dos ensaios de DPPH e de oxidação da desoxirribose, respectivamente. A capacidade quelante e redutora frente ao ferro foram determinadas através do método da orto-fenantrolina. O extrato foi analisado por HPLC quanto à presença dos ácidos gálico, clorogênico e cafeico, quercetina, rutina e kaempferol. Somente a rutina foi detectada e então usada como padrão, nas mesmas concentrações do extrato, em todos os testes. O extrato de L. divaricata (1-10 μg/ml) protegeu o cérebro contra peroxidação lipídica induzida, diminuiu os níveis basais de TBARS (± 50%) e manteve as células viáveis. O extrato não foi capaz de proteger a desoxirribose contra OH e de quelar o ferro, entretanto inibiu ON e DPPH em 33,14% a 20 μg/ml e 53,93% a 50 μg/ml respectivamente, e mostrou poder redutor de maneira concentração e tempo dependentes. Assim, o extrato etanólico das folhas de L. divaricata demonstrou propriedade antioxidante in vitro em baixas concentrações. Os mecanismos antioxidantes foram relacionados ao efeito scavenger de espécies reativas de oxigênio e de nitrogênio e à atividade redutora de metais. Esses efeitos foram similares ou superiores em comparação às mesmas concentrações de rutina, exceto na capacidade scavenger de ON e, portanto, outros compostos ainda não determinados no extrato parecem estar associados às atividades observadas neste trabalho. Mais estudos devem ser realizados em relação à identificação desses compostos e à atividade neuroprotetora do extrato.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaSoares, Félix Alexandre Antuneshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769181A8Cruz, Ivana Beatrice Mânica dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790333D6Puntel, Robson Luizhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771515A8Arantes, Leticia Priscilla2012-12-202012-12-202012-03-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfARANTES, Leticia Priscilla. In vitro ANTIOXIDANT ACTIVITY OF THE ETHANOLIC EXTRACT OF Luehea divaricata Mart. LEAVES. 2012. 62 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/11195porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-01-07T18:11:18Zoai:repositorio.ufsm.br:1/11195Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-07T18:11:18Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. In vitro antioxidant activity of the ethanolic extract of Luehea divaricata Mart. leaves |
title |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. |
spellingShingle |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. Arantes, Leticia Priscilla Açoita-cavalo Compostos fenólicos Espécies reativas de nitrogênio Espécies reativas de oxigênio Extrato vegetal Neuroproteção Açoita-cavalo Neuroprotection Phenolic compounds Plant extract Reactive oxygen species Reactive nitrogen species CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. |
title_full |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. |
title_fullStr |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. |
title_full_unstemmed |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. |
title_sort |
Atividade antioxidante in vitro do extrato etanólico das folhas de Luehea divaricata Mart. |
author |
Arantes, Leticia Priscilla |
author_facet |
Arantes, Leticia Priscilla |
author_role |
author |
dc.contributor.none.fl_str_mv |
Soares, Félix Alexandre Antunes http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769181A8 Cruz, Ivana Beatrice Mânica da http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790333D6 Puntel, Robson Luiz http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771515A8 |
dc.contributor.author.fl_str_mv |
Arantes, Leticia Priscilla |
dc.subject.por.fl_str_mv |
Açoita-cavalo Compostos fenólicos Espécies reativas de nitrogênio Espécies reativas de oxigênio Extrato vegetal Neuroproteção Açoita-cavalo Neuroprotection Phenolic compounds Plant extract Reactive oxygen species Reactive nitrogen species CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Açoita-cavalo Compostos fenólicos Espécies reativas de nitrogênio Espécies reativas de oxigênio Extrato vegetal Neuroproteção Açoita-cavalo Neuroprotection Phenolic compounds Plant extract Reactive oxygen species Reactive nitrogen species CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Oxidative stress has been linked to some neurodegenerative disorders. Current therapies are limited to attenuate the symptoms, however some studies have shown that antioxidant compounds may be able to prevent or delay neuronal oxidative damage, including those present in plant extracts. For these reasons, this study investigated the possible antioxidant activity of the ethanolic extract of Luehea divaricata leaves in brain of rats in vitro and the possible antioxidant mechanisms involved. The extract was evaluated against basal and sodium nitroprusside (SNP) 5 μM induced lipid peroxidation in brain of rats through measurements of thiobarbituric acid reactive substances (TBARS) production. Slices of brain areas were treated with SNP 100 M and extract to determine cellular viability by MTT reduction assay. Scavenger activity was evaluated against NO, DPPH and OH through Griess reagent, DPPH and deoxyribose oxidation assays, respectively. The chelating and reducing capacity for iron were determined by the orto-phenantroline method. The extract was screened by HPLC for the presence of gallic, chlorogenic, and caffeic acids, quercetin, rutin, and kaempferol. Only rutin was detected and then was used, in the same concentrations of the extract, as standard in all assays. L. divaricata extract (1-10 μg/ml) protected against induced lipid peroxidation, decreased basal levels of TBARS (about 50%) and maintained the cells viable. The extract was not able to protect deoxyribose against OH and to chelate iron, however it inhibited NO and DPPH in 33.14% at 20 μg/ml and 53.93% at 50 μg/ml respectively, and showed a reducing power in a concentration and time dependent manner. Therefore, L. divaricata ethanolic leaf extract showed antioxidant properties in vitro at low concentrations. The antioxidant mechanisms were related to scavenger activity on reactive oxygen and nitrogen species and metal reducing property. These effects were similar or more powerful than rutin in same concentrations in all assays, except in NO scavenger activity, and, thus, other unidentified compounds present in the extract appear to be associated with the effects observed in this study. More studies are needed regarding the identification of these compounds and the neuroprotective activity of the extract. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-20 2012-12-20 2012-03-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ARANTES, Leticia Priscilla. In vitro ANTIOXIDANT ACTIVITY OF THE ETHANOLIC EXTRACT OF Luehea divaricata Mart. LEAVES. 2012. 62 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. http://repositorio.ufsm.br/handle/1/11195 |
identifier_str_mv |
ARANTES, Leticia Priscilla. In vitro ANTIOXIDANT ACTIVITY OF THE ETHANOLIC EXTRACT OF Luehea divaricata Mart. LEAVES. 2012. 62 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/11195 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805930105044729856 |